| Project/Area Number |
20550085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kanagawa Institute of Technology |
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Principal Investigator |
SATOH Ikuo Kanagawa Institute of Technology, 工学部, 教授 (20148125)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Yasuhiro 神奈川工科大学, 応用バイオ科学部, 准教授 (40329305)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バイオセンサー / アポ酵素 / フロー計測 / 通電システム / 金属酵素 / 微量計測 / 重金属イオン / 固定化酵素 / 再生 / カロリメトリー / アスコルビン酸オキシダーゼ / キレート化剤 |
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| Research Abstract |
A flow-injection microdetermination system armed with an electrolytic device for trace amount of heavy metal ions was developed. A unique analytical method based on the apoenzyme activation originally created by the author was applied to realize microanalysis of heavy metal ions with use of enzymatic methods. Use of applications of 2 mA direct current across two tubular platinum electrodes set between an inlet and also outlet of the enzyme column, and also of which pH controlled at the isoelectric point, showed that removal of heavy metal ions coordinated in the active site of the enzymes was positively done and then, the enzymes could be converted to the corresponding apoenzymes. Fabrication of a flow-through reactor armed with an electrolytic device in combination with a planar type of immobilized enzymes, suffering from no increase in terminal voltage due to application of direct currents, could be successfully realized.
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