Development of chaperone-peptide beads for control of toxic nano-particle

• Project/Area Number: 20550111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Polymer chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: TANAKA Naoki Kyoto Institute of Technology, 工芸科学研究科, 准教授 (60243127)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 環境分析 / 自己組織化 / 生体材料 / 脳・神経 / ナノ毒性

Research Abstract

To develop chaperone-bead for detecting toxic nano-particle, the property of the chaperone peptide derived from the substrate binding site of the molecular chaperone αA-crystallin was studied. Chaperone peptide which corresponds to the residues 71-75 of αA-crystallin self-assemble into nano-scale fibril to efficiently reduce the aggregation of the substrate model protein. The substrate recognition mechanism of a the chaperon-peptide was studied.

Research Products

• [Journal Article] The mechanism of fibril formation of a non-inhibitory serpin ovalbumin revealed by the identification of amyloidogenic core regions (2011)
  • Author(s): Tanaka N., Morimoto Y., Noguchi Y., Tada T., Waku T., Kunugi S., Morii T., Lee Y-F., Konno T., Takahashi N.
  • Journal Title: J.Biol.Chem. 286 Pages: 5884-5889
  • Peer Reviewed
• [Journal Article] The mechanism of fibril formation of a non-inhibitory serpin ovalbumin revealed by the identification of amyloidogenic core regions (2011)
  • Author(s): N.Tanaka, Y.Morimoto, Y.Noguchi, T.Tada, T.Waku, S.Kunugi, T.Morii, Y-F.Lee, T.Konno, N.Takahashi
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 5884-5894
  • Peer Reviewed
• [Journal Article] Amyloid fibril formation and chaperone-like activity of peptides from αA-crystallin (2008)
  • Author(s): Tanaka N., Tanaka R., Tokuhara M., Kunugi S., Lee Y-F., Hamada D.
  • Journal Title: Biochemistry 47 Pages: 2961-2967
  • Peer Reviewed
• [Presentation] The mechanism of fibril formation of ovalbumin revealed by the identification of amyloidogenic core regions. (2011)
  • Author(s): Morimoto Y., Kawachi Y., Waku, T., Kunugi S., Takahashi N., Tanaka N.
  • Organizer: 第60回高分子学会年次大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-05-26
• [Presentation] ミスフォールディングを阻害するシャペロンペプチド集合体の設計 (2010)
  • Author(s): 田中直毅
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-17
• [Presentation] シャペロンペプチドの蛋白質凝集抑制におけるアミロイド線維形成の役割 (2009)
  • Author(s): 田中直毅、宮田慶亮、寺村加寿人、功刀滋
  • Organizer: 第58回高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] シャペロンペプチドにおけるアミロイド線維能と蛋白質凝集抑制機能 (2009)
  • Author(s): 田中直毅
  • Organizer: 高分子討論会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-09-16
• [Presentation] small HSP由来シヤペロンペプチドのアミロイド線維形成抑制機構 (2008)
  • Author(s): 田中直毅
  • Organizer: 高分子討論会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2008-09-24
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