Synthesis of Cello-Oligosaccharides for Alcohol Fermentation by Hydrolysis of Crystalline-Region Cellulose
| Project/Area Number |
20550116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer chemistry
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| Research Institution | Kochi University of Technology |
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Principal Investigator |
TOSHIYUKI Uryu Kochi University of Technology, 工学部, 副学長 (80011005)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASHI Yoshida 北見工業大学, 工学部, 副学長 (40166955)
小廣 和哉 高知工科大学, 工学部, 教授 (60170370)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | セルロース / 結晶領域セルロース / 高温高圧処理 / 水蒸気爆砕 / エタノール / 酸加水分解 / 結晶構造変化 / 酵素分解 / 結晶セルロース / オートクレーブ加水分解 / エタノール化 / グルコース / セロオリゴ糖 / 構造変化セルロース / ミクロクリスタリンセルロース / 加水分解 / 高温高圧水 / 遺伝子組換酵母 / 糖化 / アモルファスセルロース / 水素結合 / 木材含有糖鎖分子 |
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| Research Abstract |
A practical method to hydrolyze crystalline cellulose was found out. After crystalline cellulose suspension in water was heated at 260℃±10℃ in which temperature the hydrogen bond is weakened, it was steam-exploded. The obtained cellulose showed that hydrolysis occurred only at its surface. Mercerized cellulose showed the same phenomenon. For the crystalline cellulose converted into a different crystalline state, an acidic hydrolysis around 200℃ gave mono- and oligo-saccharide derivatives, indicating the occurrence of main-chain scission. The converted cellulose was easily hydrolyzed by enzymes into monoand oligo-saccharides.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Two-Step Cellulose Hydrolysis Using Modest Amount of Concentrated Sulfuric Acid or Autoclave and Fermentation of the Hydrolyzates into Ethanol Utilizing Recombinant Yeast2010
Author(s)
T. Uryu, K. Katsuraya, K. Okuyama, H. Kato, C. Uchiyama, A. Oka, N. Kubo, M. Takahashi, M. Kudo, M. Kawabe, T. Kobayashi, S. Ohnishi, M. Aida, K. Shishikura, H. Iefuji, K. Kobiro, Y. Tsutsui, T. Yoshida
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Journal Title
Bulletin of Kochi University of Technology 7
Pages: 101-108
NAID
Related Report
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[Journal Article] Effects of Lacquer Polysaccharides, Glycoproteins and Isoenzymes on the Activity of Free and Immobilized Laccase fromRhus vernicifera2010
Author(s)
Y. Wan, R. Lu, K. Akiyama, K. Okamoto, T. Honda, Y. Du, T. Yoshida, T. Miyakoshi, C. J. Knill, J. F. Kennedy
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Journal Title
Intern.J.Biol.Macromol. 47
Pages: 76-81
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