On-chip cell-free genetic engineering as a research technology for proteomics
| Project/Area Number |
20550148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NOJIMA Takahiko The University of Tokyo, 一般教育部, 講師 (00291930)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII Teruo 東京大学, 生産技術研究所, 教授 (30251474)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNA / GFP / PCR / キャピラリー電気泳動 / タンパク質 / プロテオミクス / 翻訳 / 遺伝情報発見 / 蛍光蛋白質 / プライマー / 分子システム / 増幅反応 / 論理ゲート / 論理判断 / RNA / アミノ酸 / 蛋白質 / 転写 / 遺伝子 / 遺伝子工学 / マイクロ流体チップ / 蛍光イメージング / キャピラリ-電気泳動 |
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| Research Abstract |
An experimental system has been developed to achieve recombinant DNA and gene expression without living cell by enzymatic reactions and on-chip electrophoresis of DNA.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Electrophoresis2010
Author(s)
Shohei Kaneda, Koichi Ono, Tatsuhiro Fukuba, Takahiko Nojima, Takatoki Yamamoto, Teruo Fujii
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Journal Title
Vol.31, No.22
Pages: 3719-3726
Related Report
Peer Reviewed
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