| Project/Area Number |
20550149
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
TANAKA Hiroshi Tokyo Institute of Technology, 大学院・理工学研究科, 准教授 (40334544)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OHNO Naohito 東京薬科大学, 薬学部, 教授 (80152213)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 自然免疫 / デクチン-1 / オリゴ糖 / グリコシル化反応 / 糖質 / グリコシル化 / デクチン1 / βグルカン / 多糖 / 免疫 / 固相合成 |
|---|
| Research Abstract |
In this report, we describe the synthesis and biological evaluation of β (1,3) oligosaccharides possessing an aminoalkyl group and their biological evaluation. A 2,3 diol glycoside with a 4,6 benziliden protecting group finctioned as an effective glycosyl acceptor for the synthesis ofβ ・1,3)linked glycosides. Using a combination of a linear tetrasaccharide and a branched pentasaccharide as glycosyl donors permitted β ・1,3)linear octa-to hexadecasaccharides and branched nona- to heptadecasaccharides to be prepared in good total yields. Measurements of the competitive effects of oligosaccharides on the binding of a soluble dectin-1 to a solid-supported Schizophyllan (SPG) revealed that the branched heptadecasacccharide and the linear hexadecasacccharides 2 also have binding activity for to dectine-1. In addition, the two oligosaccharides exhibited agonist activity in a luciferase-assisted NF-kB assay. This represents the first demonstration of a purely synthetic oligosaccahrides stimulating immunity mediated by dectin-1.
|
