| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
In this study we have studied the improvement of the catalytic antibodies by newly devised heterologous immunization. We have developed a method to improve the molecular recognition of the catalytic antibody in antigen-combining site. In heterologous immunization, in which acetyl-transition-state analog was injected into mice after injection of trifluoroacetyl-transition-state analog, catalytic antibodies with higher hydrolytic activities were obtained. The kinetic parameters and amino acid sequences were determined to discuss the catalytic mechanism of the catalytic antibodies.
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