Development of tailor-made catalyst (catalytic antibody) by heterologous immunization
Project/Area Number |
20550155
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
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Research Institution | Osaka Prefecture University |
Principal Investigator |
TSUMURAYA Takeshi Osaka Prefecture University, 理学系研究科, 准教授 (00372855)
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Co-Investigator(Kenkyū-buntansha) |
FUJII Ikuo 大阪府立大学, 理学系研究科, 教授 (70189984)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 酵素 / モノクローナル抗体 / 抗体酵素 / 免疫学 / タンパク質 / 生体分子 / 遷移状態アナログ / 抗原 / ハイブリドーマ / 免疫 / 進化分子工学 / ライブラリー |
Research Abstract |
In this study we have studied the improvement of the catalytic antibodies by newly devised heterologous immunization. We have developed a method to improve the molecular recognition of the catalytic antibody in antigen-combining site. In heterologous immunization, in which acetyl-transition-state analog was injected into mice after injection of trifluoroacetyl-transition-state analog, catalytic antibodies with higher hydrolytic activities were obtained. The kinetic parameters and amino acid sequences were determined to discuss the catalytic mechanism of the catalytic antibodies.
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Report
(4 results)
Research Products
(90 results)
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K. Tsumoto, A. Yokota, Y. Tanaka, M. Ui, T. Tsumuraya, I. Fujii, I. Kumagai, Y. Nagumo, H. Oguri, M. Inoue, M. Hirama
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Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
Kouhei Tsumoto, Akiko Yokota, Yoshikazu Tanaka, Mihoko Ui, Takeshi Tsumuraya, Ikuo Fujii, Izumi Kumagai, Yoko Nagumo, Hiroki Oguri, Masayuki Inoue, Masahiro Hirama
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Journal Title
Journal of Biological Chemistry 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Presentation] ポロ酵素型触媒抗体の分子設計2010
Author(s)
廣瀬仁孝, 石川文洋, 円谷健, 藤井郁雄
Organizer
第27回有機合成化学セミナー
Place of Presentation
シーサイドホテル舞子ビラ 神戸(兵庫県)
Year and Date
2010-09-03
Related Report
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[Presentation] ホロ酵素型抗体酵素2009
Author(s)
石川文洋, 円谷健, 藤井郁雄
Organizer
2009年度酵素・補酵素研究会
Place of Presentation
奈良
Year and Date
2009-07-10
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