| Project/Area Number |
20550156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | University of Hyogo |
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Principal Investigator |
YAMANA Kazushige University of Hyogo, 大学院・工学研究科, 教授 (70192408)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Mitsunobu 兵庫県立大学, 大学院・工学研究科, 准教授 (50285342)
TAKADA Tadao 兵庫県立大学, 大学院・工学研究科, 助教 (60511699)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 生体機能材料 / DNA / 電気化学検出 / 一塩基変異検出 / 核酸アプタマー / レドックスレポーター / 遺伝子タイピング / DNAデバイス / レドックス修飾DNA / 鎖交換反応 / 遺伝子 / タイピング / バイオセンサー |
|---|
| Research Abstract |
We have prepared several DNA devices that involve anthraquinone or ferrocene as a redox reporter at the specific position of DNA. The devices exhibit excellent performances in the sensing of DNA sequences or biomolecules such as ATP and proteins. Our method for the DNA sensing is based on strand exchange reactions of the redox-probe oligonucleotide with target DNA at the surface of the devices. We have shown that a strand exchange approach is applicable in aptamer based biosensors. During the grant research period, we have also succeeded the synthesis of redox-oligonucleotides via the pai-electron conjugation between the reporter and the nucleoside base. We anticipate that the redox-oligomers would be used for the new systems in detection of DNA mutation and in electron migration on DNA wires.
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