| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The aim of present study was to reveal enzymatic properties of three types belonged to a family of prolyl oligopeptidase in Streptomyces genomes. The evidence presented in this study demonstrates that one is an exo-aminopeptidase, and the other is an endo-peptidase. The later shows an trypsin-like activity, and is belonged to a family of oligopeptidase B. Furthermore, by substitution of the active serine residue in the aminopeptidase with cysteine, its aminolysis activity is more enhanced than that of the wild-type enzyme.
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