Construction of Artificial Biocatalysts containing a Nonnatural Dinuclear Active Site
| Project/Area Number |
20559002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
ONODA Akira 大阪大学, 工学研究科, 助教 (60366424)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | 金属タンパク質 / 多核金属錯体 / 鉄二核中心 / 人工タンパク質 / 酸化反応 / 進化工学 / 酵素触媒 |
|---|
| Research Abstract |
Artificial metalloenzymes containing unnatural multinuclear core were constructed in this project. Dioxygen-binding diiron protein, DcrH-Hr, was studied to elucidate the characteristics and the approaches using a mutation of amino acid residues around the active site led to conversion of the oxygen-binding function. Successful incorporation of a diiron hexacarbonyl core into the protein matrix of cytochrome c provided us artificial hydrogenase capable of photocatalytic hydrogen evolution in an aqueous solution.
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Report
(4 results)
Research Products
(53 results)
