| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study, influence of the contact state of cells in the sample of tissue level as high cell density on a plasma-membrane injury in a freeze-thaw process was investigated using the monolayer-culture cell which controlled the orientation and the cell density. Furthermore, feasibility of the improvement in cell viability by the hydrate near the plasma membrane of the hyaluronan which is a moisturizer was investigated. As a result, it was suggested that a plasma-membrane injury increased with increasing the cell density, and that the extent of an injury depended on a cell orientation and freezing sequences. Moreover, it is clear that hyaluronan has cryoprotective effect for plasma-membrane.
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