The evaluation of plasma-membrane injury and the effect of a moisturizer on injury reduction in freezing of tissue level
| Project/Area Number |
20560197
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thermal engineering
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| Research Institution | Kitasato University |
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Principal Investigator |
UJIHIRA Masanobu Kitasato University, 医療衛生学部, 准教授 (70286392)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 生体熱工学 / 生体組織の凍結保存 / ヒアルロン酸 / 凍結保護効果 / 単層培養細胞 / 細胞の配向制御 / ヒト皮膚繊維芽細胞 / ヒアルロン酸ナトリウム / 凍結方向 / 蛍光染色 |
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| Research Abstract |
In this study, influence of the contact state of cells in the sample of tissue level as high cell density on a plasma-membrane injury in a freeze-thaw process was investigated using the monolayer-culture cell which controlled the orientation and the cell density. Furthermore, feasibility of the improvement in cell viability by the hydrate near the plasma membrane of the hyaluronan which is a moisturizer was investigated. As a result, it was suggested that a plasma-membrane injury increased with increasing the cell density, and that the extent of an injury depended on a cell orientation and freezing sequences. Moreover, it is clear that hyaluronan has cryoprotective effect for plasma-membrane.
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Report
(4 results)
Research Products
(21 results)
