Enrichment of Marine anammox bacteria and application to nitrogen removal technology in a high-salinity water

• Project/Area Number: 20560508
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Kumamoto University
• Principal Investigator: KAWAGOSHI Yasunori Kumamoto University, 大学院・自然科学研究科, 准教授 (00291211)
• Co-Investigator(Renkei-kenkyūsha): FURUKAWA Kenji 熊本大学, 大学院・自然科学研究科, 教授 (60029296)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 環境保全 / 環境微生物 / 窒素除去 / 嫌気性アンモニア酸化(Anammox) / 海洋性Anammox / 海洋性Anammox細菌 / 集積培養

Research Abstract

The aim of this study is construction of enrichment culture of marine anammox bacteria for the development of novel nitrogen removal technology which is robust to fluctuation of environmental condition such as temperature or salinity. We succeeded the enrichment cultivation of marine anammox bacteria by using the sediment of a sea-based municipal waste disposal site as the inoculum. The enrichment culture performed stable nitrogen removal ability even under the condition of 5% salinity and less than 25℃ temperature. From bacterial community analysis, marine anammox bacteria closely related to Canidatus Scalindua and other bacteria such as sulfar-oxidizers were detected in the culture.

Research Products

• [Journal Article] Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study. (2010)
  • Author(s): Kawagoshi Y., Nakamura Y., Kawashima H., Fujisaki K., Furukawa K., Fujimoto A.
  • Journal Title: Water Sci.Technol. 61(1) Pages: 119-126
• [Journal Article] Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study (2010)
  • Author(s): Yasunori Kawagoshi, et al.
  • Journal Title: Water Science and Technology Volume: 61 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study (2010)
  • Author(s): Yasunori Kawagoshi, et al.
  • Journal Title: Water Science and Technology doi:10.2166 Pages: 119-126
  • Peer Reviewed
• [Journal Article] Enrichment culture of marine anaerobic ammonium oxidation (anammox) bacteria from sediment of sea-based waste disposal site. (2009)
  • Author(s): Kawagoshi Y., Nakamura Y., Kawashima H., Fujisaki K., Fujimoto A., Furukawa K.
  • Journal Title: J.Biosci.Bioeng. 107(1) Pages: 61-63
  • NAID: 110007042022
• [Journal Article] Enrichment culture of marine anaerobic ammonium oxidation(anammox)bacteria from sediment of sea-based waste disposalsite. (2009)
  • Author(s): Yasunori Kawagoshi
  • Journal Title: Journal of Bioscience and Bioengineering 107 Pages: 61-63
  • Peer Reviewed
• [Presentation] 海洋性Anammox培養系における温度と塩分濃度の影響 (2011)
  • Author(s): 山城健人, 友重勇気, Luong Van Duc, 〓巧〓 川越保徳
  • Organizer: 平成22年度日本水環境学会九州支部研究発表会講演概要集,p.9
  • Place of Presentation: 福岡大学
  • Year and Date: 2011-03-14
• [Presentation] 海洋性Anammox培養系における温度と塩分濃度の影響 (2011)
  • Author(s): 山城健人, 友重勇気, 川越保徳, 他
  • Organizer: 平成22年度日本水環境学会九州支部研究発表会
  • Place of Presentation: 福岡大学
  • Year and Date: 2011-03-14
• [Presentation] 海洋性Anammox培養系への温度と塩分濃度の影響 (2011)
  • Author(s): 山城健人, 川越保徳, 友重勇気
  • Organizer: 平成22年度土木学会西部支部研究発表会講演概要集(CD/ROM),VII-12
  • Place of Presentation: 九州工業大学
  • Year and Date: 2011-03-05
• [Presentation] 海洋性Anammox培養系への温度と塩分濃度の影響 (2011)
  • Author(s): 山城健人, 川越保徳, 他
  • Organizer: 平成22年度土木学会西部支部研究発表会
  • Place of Presentation: 九州工業大学
  • Year and Date: 2011-03-05
• [Presentation] 海洋性Anammox細菌の集積培養系における窒素除去能と細菌叢の温度特性 (2010)
  • Author(s): 友重勇気, 朱文呈, 藤崎幸一郎, 川越保徳
  • Organizer: 日本水処理生物学会第47回大会,日本水処理生物学会誌,別巻30号,p.16
  • Place of Presentation: つくば大学
  • Year and Date: 2010-11-19
• [Presentation] 海洋性Anammox細菌の集積培養系における窒素除去能と細菌叢の温度特性 (2010)
  • Author(s): 友重勇気, 川越保徳, 他
  • Organizer: 日本水処理生物学会第47回大会
  • Place of Presentation: つくば大学
  • Year and Date: 2010-11-18
• [Presentation] 海洋性 Anammox 細菌集積リアクタにおける窒素除去能に対する温度とリアクタ形状の影響 (2010)
  • Author(s): 友重勇気, 川越保徳, 他
  • Organizer: 平成21年度日本水環境学会九州支部研究発表会
  • Place of Presentation: 北九州市立大学
  • Year and Date: 2010-03-18
• [Presentation] 海洋性 Anammox 細菌を用いた窒素除去の高速化に向けた培養条件の検討 (2010)
  • Author(s): 友重勇気, 川越保徳, 他
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡大学
  • Year and Date: 2010-03-17
• [Presentation] 海洋性Anammox細菌を用いた窒素除去の高速化に向けた培養条件の検討 (2010)
  • Author(s): 友重勇気, 糸密尚貴, 佐藤宇紘, 川越保徳
  • Organizer: 第44回日本水環境学会年会講演集pp.15-17
  • Place of Presentation: 福岡大学
  • Year and Date: 2010-03-16
• [Presentation] 海洋性 Anammox 細菌集積リアクタにおける窒素除去性能の向上に関する基礎的研究 (2010)
  • Author(s): 友重勇気, 川越保徳, 他
  • Organizer: 平成21年度土木学会西部支部研究発表会
  • Place of Presentation: 崇城大学
  • Year and Date: 2010-03-05
• [Presentation] Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study (2009)
  • Author(s): Kawagoshi Y., Nakamura Y., Kawashima K., Fujisaki K., Furukawa K., Fujimoto A.
  • Organizer: 6th IWA Leading-Edge Conference on Water and Wastewater Technologies
  • Place of Presentation: Singapore,Suntec Singapore International Convention and Exhibition Centre(CD-ROM)
  • Year and Date: 2009-06-24
• [Presentation] 海洋性Anammox細菌の集積培養に対する温度及び培地条件の検討 (2009)
  • Author(s): 藤崎幸市郎
  • Organizer: 第43回日本水環境学会年会
  • Place of Presentation: 山口大学
  • Year and Date: 2009-03-18
• [Presentation] 海洋性Anammox細菌の集積培養に対する温度及び培地条件の影響 (2009)
  • Author(s): 藤崎幸市郎, 野村太一, 藤本綾, 川越保徳
  • Organizer: 第43回日本水環境学会年会,第43回日本水環境学会年会講演集,p.425
  • Place of Presentation: 山口大学
  • Year and Date: 2009-03-17
• [Presentation] 海洋性Anammox細菌の集積培養条件に関する研究 (2009)
  • Author(s): 野村太一, 藤崎幸市郎, 藤本綾, 川越保徳
  • Organizer: 平成20年度土木学会西部支部研究発表会,平成20年度土木学会西部支部研究発表会講演概要集,pp.997-998
  • Place of Presentation: 九州大学
  • Year and Date: 2009-03-07
• [Presentation] 海洋性Anammox細菌の集積培養条件に関する研究 (2009)
  • Author(s): 野村太一
  • Organizer: 平成20年度土木学会西部支部研究発表会
  • Place of Presentation: 九州大学
  • Year and Date: 2009-03-07
• [Presentation] 海洋性Anammox細菌の集積培養に対する温度及び培地条件の影響 (2009)
  • Author(s): 藤崎幸市郎
  • Organizer: 平成20年度日本水環境学会九州支部研究発表会
  • Place of Presentation: 熊本大学
  • Year and Date: 2009-02-28
• [Presentation] Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study (2009)
  • Author(s): 川越保徳, et al.
  • Organizer: 6^<th> IWA Leading-Edge Conference on Water and Wastewater Technologies
  • Place of Presentation: Singapore