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Efficient production of manganese peroxidase by the optimization of growth morphology

Research Project

Project/Area Number 20560727
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionUniversity of Fukui

Principal Investigator

SAKURAI Akihiko  University of Fukui, 大学院・工学研究科, 准教授 (40283163)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsバイオ生産プロセス / マンガンペルオキシダーゼ / 白色腐朽菌 / ペレット / 増殖形態 / リグニン分解酵素 / 蛍光染色 / ダイオキシン / 遺伝子発現
Research Abstract

Ligninolytic enzymes are expected as an agent for the degradation of environmental pollutant and/or for the pretreatment of cellulolytic biomass. The purpose of this study was to produce high amount of manganese peroxidase, a kind of ligninolitic enzymes, by the optimization of growth morphology of white rot fungus. The expression level of manganese peroxidase gene was high when the diameter of mycelial pellet was below 0.7 mm.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2010 2009 2008

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (15 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Production of manganese peroxidase by white rot fungi from potato-processing wastewater : Role of amino acids on biosynthesis.2010

    • Author(s)
      S.Fujihara, M.Hatashita, A.Sakurai, M.Sakakibara
    • Journal Title

      African J.Biotechnol. 9

      Pages: 725-731

    • Related Report
      2010 Final Research Report
  • [Journal Article] Production of manganese peroxidase by white rot fungi from potato-proc essing wastewater : Role of amino acids on biosynthesis.2010

    • Author(s)
      S.Fujihara, M.Hatashita, A.Sakurai, M.Sakakibara
    • Journal Title

      African J.Biotechnol

      Volume: 9 Pages: 725-731

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of manganese peroxidase by white rot fungi from potato-processing wastewater : Role of amino acids on biosynthesis2010

    • Author(s)
      S.Fujihara, M.Hatashita, A.Sakurai, M.Sakakibara
    • Journal Title

      African J.Biotechnol 9

      Pages: 725-731

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimization of medium components for manganese peroxidase production by white rot fungus strain2008

    • Author(s)
      S.Fujihara, A.Sakurai, M.Sakakibara
    • Journal Title

      L-25.J.Chem.Eng.Jpn. 41

      Pages: 796-803

    • Related Report
      2010 Final Research Report
  • [Journal Article] Optimization of medium components for manganese peroxidase production by white rot funeus strain L-252008

    • Author(s)
      S. Fujihara, A. Sakurai, M. Sakakibara
    • Journal Title

      Journal of Chemical Engineering of Japan 41

      Pages: 796-803

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] イオンビーム照射による白色腐朽菌の育種2010

    • Author(s)
      纐纈由香菜, 藤原伸哉, 畑下昌範, 櫻井明彦
    • Organizer
      第62回日本生物工学会大会
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] マンガンペルオキシダーゼ生産における遺伝子発現及び細胞外タンパク質の解析2010

    • Author(s)
      藤原伸哉, 纐纈由香菜, 櫻井 明彦, 畑下昌範
    • Organizer
      第62回日本生物工学
    • Related Report
      2010 Final Research Report
  • [Presentation] Production of manganese peroxidase by Bjerkandera sp.mutant induced by ion beam irradiation2010

    • Author(s)
      A.Sakurai, Y.Koketsu, S.Fujihara, M.Hatashita
    • Organizer
      IBS2010proceedings
    • Related Report
      2010 Final Research Report
  • [Presentation] ビーム照射による白色腐朽菌の高性能化2010

    • Author(s)
      纐纈由香菜, 櫻井明彦, 榊原 三樹男, 畑下昌範
    • Organizer
      化学工学会第75年会
    • Related Report
      2010 Final Research Report
  • [Presentation] Production of manganese peroxidase by Bjerkandera sp. mutant induced by ion beam irradiation2010

    • Author(s)
      A.Sakurai, Y.Koketsu, S.Fujihara, M.Hatashita
    • Organizer
      IBS2010
    • Place of Presentation
      Rimini, Italy
    • Related Report
      2010 Annual Research Report
  • [Presentation] マンガンペルオキシダーゼ生産における遺伝子発現及び細胞外タンパク質の解析2010

    • Author(s)
      藤原伸哉, 纐纈由香菜, 櫻井明彦. 畑下昌範
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression analysis of manganese peroxidase gene in white rot fungus L-25 in the presence of amino acids2009

    • Author(s)
      S.Fujihara, A.Sakurai, M.Sakakibara
    • Organizer
      APBioChEC09
    • Place of Presentation
      神戸
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 白色腐朽菌によるリグニン分解酵素生産と有害物質処理2009

    • Author(s)
      櫻井明彦
    • Organizer
      鳥取大学工学部生物応用工学特別セミナー
    • Place of Presentation
      鳥取大学
    • Year and Date
      2009-09-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression analysis of manganese peroxidase gene in white rot fungus L-25 in the presence of amino acids2009

    • Author(s)
      藤原伸哉, 櫻井明彦, M.Sakakibara
    • Organizer
      APBioChEC09 proceedings
    • Related Report
      2010 Final Research Report
  • [Presentation] Effect of Amino Acids on Manganese Peroxidase Production2009

    • Author(s)
      S.Fujihara, A.Sakurai, M.Sakakibara
    • Organizer
      8th World Congress of Chemical Engineering
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 白色腐朽菌における増殖形態とマンガンペルオキシダーゼ生産性の関係2008

    • Author(s)
      橋詰直幸, 楼井明彦, 榊原三樹男
    • Organizer
      化学工学会新潟大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2008-08-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 白色腐朽菌L-25株のマンガンペルオキシダーゼ生産に与えるアミノ酸の影響2008

    • Author(s)
      藤原伸哉, 楼井明彦, 榊原三樹男
    • Organizer
      化学工学会新潟大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2008-08-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 白色腐朽菌L-25 株のマンガンペルオキシダーゼ生産に与えるアミノ酸の影響2008

    • Author(s)
      藤原伸哉, 櫻井明彦, 榊原三樹男
    • Organizer
      化学工学会新潟大会
    • Related Report
      2010 Final Research Report
  • [Presentation] 白色腐朽菌における増殖形態とマンガンペルオキシダーゼ生産性の関係2008

    • Author(s)
      橋詰直幸, 櫻井明彦, 榊原三樹男
    • Organizer
      化学工学会新潟大会
    • Related Report
      2010 Final Research Report
  • [Presentation] 染色排水からのダイオキシン類低減化試験について2008

    • Author(s)
      神戸真暁, 熊谷宏之, 南保幸男, 櫻井明彦
    • Organizer
      第17回環境化学討論会
    • Related Report
      2010 Final Research Report
  • [Book] 酵素利用技術大系 分担:廃水処理(フェノール廃液)2010

    • Author(s)
      小宮山 眞監修
    • Publisher
      NTS
    • Related Report
      2010 Final Research Report
  • [Book] 酵素利用技術大系分担:廃水処理(フェノール廃液)2010

    • Author(s)
      小宮山眞監修
    • Total Pages
      993
    • Publisher
      NTS
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 難分解性物質の分解菌およびそれを用いた環境の浄化方法2009

    • Inventor(s)
      櫻井明彦
    • Industrial Property Rights Holder
      福井大学
    • Filing Date
      2009-01-23
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 特許2009

    • Inventor(s)
      櫻井明彦
    • Industrial Property Rights Holder
      福井大学
    • Acquisition Date
      2009-01-23
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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