IR spectroscopic analysis on sugar metabolic kinetics of suspension plant cells for efficient utilization of biomass materials

• Project/Area Number: 20560729
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Mie University
• Principal Investigator: SUEHARA Ken-ichiro Mie University, 大学院・生物資源学研究科, 准教授 (70291614)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Atsushi 三重大学, 大学院・生物資源学研究科, 教授 (40242937)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 懸濁植物細胞 / 赤外・近赤外分光法 / 再資源化糖 / バイオマス / 植物細胞培養 / 糖代謝 / 赤外分光法 / 速度論的解析 / バイオマス由来の糖

Research Abstract

The objective of this work was to study the influence of sugar spices in the culture media on the kinetic sugar uptake phenomena for three types of plant cell cultivation. We developed the measurement method of sugar content in culture media suspended plant cells using the FT-IR/ATR method, and the sugar uptake rate of the suspension plant cells were calculated. The sugar uptake rate of the plant cell was different greatly on the kind of sugar, however, characteristic of sugar metabolism based on cell growth period was stable. Characteristic of metabolism of various sugars and sugar mixture (that is biomass degradation product) for three types of plant cells were obtained. These results will contribute to the development of a plant cell cultivation process planning and bioprocess monitoring by IR method to utilize the sugar derived from biomass materials.

Research Products

• [Journal Article] Infrared spectral feature of plant-cell culture media (2010)
  • Author(s): K Suehara, A Hashimoto
  • Journal Title: Proceedings of 14th international conference of near infrared spectroscopy Pages: 1141-1145
  • Peer Reviewed
• [Presentation] 中赤外分光法を援用した懸濁植物細胞の糖代謝挙動に及ぼすグルコース前培養の影響評価 (2011)
  • Author(s): 末原憲一郎・川口摩悠美・亀岡孝治・橋本篤
  • Organizer: 化学工学会第72年会
  • Place of Presentation: 小金井
  • Year and Date: 2011-03-22
• [Presentation] 中赤外分光法を援用した懸濁植物細胞の糖代謝挙動に及ぼすグルコース前培養の影響評価 (2011)
  • Author(s): 末原憲一郎・川口摩悠美・亀岡孝治・橋本篤
  • Organizer: 化学工学会第72年会
  • Place of Presentation: 東京都小金井市(東京農工大)
  • Year and Date: 2011-03-22
• [Presentation] 中赤外分光法を援用した懸濁アラビドプシス細胞の糖代謝挙動の速度論的検討 (2010)
  • Author(s): 末原憲一郎・江川芳美・酒井麻衣・亀岡孝治・橋本篤
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-08
• [Presentation] 中赤外分光法を援用した懸濁アラビドプシス細胞の糖代謝挙動の速度論的検討 (2010)
  • Author(s): 末原憲一郎・江川芳美・酒井麻衣・亀岡孝治・橋本篤
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都(同志社大学)
  • Year and Date: 2010-09-08
• [Presentation] Grasping of Spectral Feature on MIR and NIR Regions for Analysis of Plant-cell Culture Broth (2009)
  • Author(s): 末原憲一郎, 橋本篤
  • Organizer: International conference of near infrared spectroscopy(NIR-2009)
  • Place of Presentation: タイ・バンコク
  • Year and Date: 2009-11-10
• [Presentation] Grasping of Spectral Feature on MIR and NIR Regions for Analysis of Plant-cell Culture Broth (2009)
  • Author(s): 末原憲一郎
  • Organizer: 第14回近赤外分光国際会議
  • Place of Presentation: タイ・バンコク
  • Year and Date: 2009-11-09