| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
The objective of this work was to study the influence of sugar spices in the culture media on the kinetic sugar uptake phenomena for three types of plant cell cultivation. We developed the measurement method of sugar content in culture media suspended plant cells using the FT-IR/ATR method, and the sugar uptake rate of the suspension plant cells were calculated. The sugar uptake rate of the plant cell was different greatly on the kind of sugar, however, characteristic of sugar metabolism based on cell growth period was stable. Characteristic of metabolism of various sugars and sugar mixture (that is biomass degradation product) for three types of plant cells were obtained. These results will contribute to the development of a plant cell cultivation process planning and bioprocess monitoring by IR method to utilize the sugar derived from biomass materials.
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