| Project/Area Number |
20560732
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Sojo University |
|---|
Principal Investigator |
MATSUMOTO Yoko Sojo University, 生物生命学部, 教授 (00133562)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
GOTO Koichi 崇城大学, 生物生命学部, 准教授 (30279377)
ICHIHARA Hideaki 崇城大学, 生物生命学部, 助教 (70369114)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | リポソーム(複合脂質膜) / がん化学療法 / 糖 / 脂質 / アポトーシス / 細胞膜 |
|---|
| Research Abstract |
Hybrid liposomes (HL) can be prepared by sonication of vesicular and micellar molecules in a buffer solution. The physical properties of HL such as size, shape, membrane fluidity, and the temperature of phase separation can be controlled by changing the constituents and compositional ratio. We have employed three-component hybrid liposomes (THL) composed of L-α-dimyristoylphosphatidylcholine (DMPC), polyoxyethylene sorbitane monolaurate (Tween 20), and decyl-β-lactopranoside (LactC_<10>) (DMPC : Tween 20 : LactC_<10> = 65 : 7 : 28) for the research of cancers and interesting results are as follows;
(A) LactC_<10> was successfully produced from decyl-β-lactoheptaacetate (Ac-LactC_<10>), which was obtained by glycosylation and deacetylation of β-lactoseoctaacetate (Ac-Lact).
(B) IC50 values of three-component hybrid liposomes including lactose surfactants (HL/LactC_<10>) for human hepatoma (Hep-G2, Huh-7) cells were smaller than those of two-component hybrid liposomes (HL).
(C) The thickness of fixed aqueous layer (TFAL) of HL/LactC_<10> was about twice that of HL. The result suggests that the inhibitory effects of HL/LactC_<10> could be related to hydration in tumor cells.
(D) Induction of apoptosis by HL/LactC_<10> for Hep-G2 and Huh-7 cells were obtained.
Thus, this study demonstrated the importance of relation between hydration and inhibition of the growth of hepatoma cells using THL having specific fixed aqueous layers.
|
