| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
OsPCL1 is unique gene in the rice genome and homologous to the Arabidopsis clock gene PHYTOCLOCK1 (PCL1). The gene should be controlling the photoperiodic flowering in rice. We screened mutants containing mutations on OsPCL1 locus by TILLING. In ～3,600 EMS (ethylmethan sulfonate) mutated rice lines (M_3 or M_4), we isolated 7 OsPCL1 mutants. Three mutants had single nucleotide substitution in the coding sequence of OsPCL1 causing single amino acid substitution in OsPCL1 but showed little effects on the photoperiodic flowering and circadian gene expressions. Residual 4 mutants had silent mutation. These results suggest that more mutants are needed to clarify the gene functions. We have prepared OsPCL1 RNAi lines in rice and are investigating their phenotypes.
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