Project/Area Number |
20570002
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genetics/Genome dynamics
|
Research Institution | Nagoya University |
Principal Investigator |
ONAI Kiyoshi Nagoya University, 遺伝子実験施設, 研究員 (00402454)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 生物時計 / 概日リズム / 生物発光 / 時計遺伝子 / PCL1 / PHYTOCLOCK / 光周性 / 高等植物 / PHYTOCLOCK1 |
Research Abstract |
OsPCL1 is unique gene in the rice genome and homologous to the Arabidopsis clock gene PHYTOCLOCK1 (PCL1). The gene should be controlling the photoperiodic flowering in rice. We screened mutants containing mutations on OsPCL1 locus by TILLING. In ~3,600 EMS (ethylmethan sulfonate) mutated rice lines (M_3 or M_4), we isolated 7 OsPCL1 mutants. Three mutants had single nucleotide substitution in the coding sequence of OsPCL1 causing single amino acid substitution in OsPCL1 but showed little effects on the photoperiodic flowering and circadian gene expressions. Residual 4 mutants had silent mutation. These results suggest that more mutants are needed to clarify the gene functions. We have prepared OsPCL1 RNAi lines in rice and are investigating their phenotypes.
|
Report
(4 results)
Research Products
(107 results)
-
[Journal Article] Plasma membrane aquaporin agpZ is essential for glucose metabolism during photomixotrophic growth of Synechocysiis sp. PCC 6803.2011
Author(s)
Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N
-
Journal Title
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Functionally important structural elements of the cyanobacterial clock-related protein Pex.2009
Author(s)
Kurosawa S, Murakami R, Onai K(共著第一著者), Morishita M, Hasagawa D, Iwase R, Uzumaki T, Hyashi F,Kitajima-Ihara T, Sakata S, Mrakami M, Kouyama T, Ishiura M
-
Journal Title
Genes Cells 14
Pages: 1-16
Related Report
Peer Reviewed
-
[Journal Article] Identification and characterization of the Na+/H+ antiportere NhaS3 from the thylakoid mambrane of Synechocystis sp.PCC 6803.2009
Author(s)
Tsunekawa K, Shijyuku T, Hayashimoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N
-
Journal Title
J Biol Chem 284
Pages: 16513-16521
Related Report
Peer Reviewed
-
-
[Journal Article] Functionally important structural elements of the cyano bacterial clock-related protein Pex.2009
Author(s)
Kurosawa S., Murakami R., Onai K. (共著第一著者), Morishita M., Hasegawa D., Iwase R., Uzumaki T., Hayashi F., Kitajima-Ihara T., Sakata S., Murakami M., Kouyama T., Ishiura M.
-
Journal Title
Genes to Cells 14
Pages: 1-16
Related Report
Peer Reviewed
-
[Journal Article] Identification and characterization of the Na+/H+ antiporter NhaS3 from the thylakoid membrane of Synechocystis sp. PCC 6803.2009
Author(s)
Tsunekawa K., Shijyuku T., Hayashimoto M., Kojima Y., Onai K., Morishita M., Ishiura M., Kuroda T., Nakamura T., Kobayashi H., Sato M., Toyooka K., Matsuoka K., Omata T., Uozumi N.
-
Journal Title
Journal of Biological Chemistry (印刷中)
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Large scale screening of Arabidopsis mutants in seed oil synthesis by using a high-throughput real-time bioluminescence monitoring system.2010
Author(s)
Kawai T, Onai K, Hashimoto M, Maeo K, Ishiura M, Nakamura K
Organizer
2nd Gordon Research Conference on "Plant Lipids, Structure, Metabolism & Function" (GRC on Plant Lipids), Oral
Place of Presentation
Hotel Galvez, Galveston, Texas, USA
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 藍色細菌の生物時計関連タンパク質Pexの構造と機能の解析2008
Author(s)
村上伶子, 黒澤俊介, 小内清, 森下めぐみ, 宇津巻竜也, 林史夫, 岩瀬亮, 武藤梨沙, 佐藤洋志, 長谷川大祐, 神山勉, 石浦正寛
Organizer
日本光合成研究会公開シンポジウム
Place of Presentation
名古屋大学野依記念学術交流館、名古屋
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-