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Transcriptome analysis to understand the mechanisms of the restarting of bacterial growth after the stationary phase.

Research Project

Project/Area Number 20570006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionOsaka Medical College

Principal Investigator

MAKI Yasushi  Osaka Medical College, 医学部, 講師 (60401733)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords細菌 / マイクロアレイ / 増殖 / 大腸菌
Research Abstract

To reveal the mechanisms to restart the proliferation of the bacterial cells from the growth suspending, I have performed the microarray analysis of the cells of various states through the cell cycle, using Escherichia coli cells as the model bacteria in this study. The results showed that many genes are transcribed in the early period of the restarting growth after the inoculation of the cells of stationary phase into the fresh medium, and that the physiological states, as well as the profiles of the gene expressions, of the cells of nonproliferation periods are varying according to the nutrient environments.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2011 2010 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (12 results) Remarks (4 results)

  • [Journal Article] Structure of the 100S Ribosome in the Hibernation Stage Revealed by Electron Cryomicroscopy.2010

    • Author(s)
      Takayuki Kato, Hideji Yoshida, Tomoko Miyata, Yasushi Maki, Akira Wada, Keiichi Namba
    • Journal Title

      Structure vol.18

      Pages: 719-724

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure of the 100S Ribosome in the Hibernation Stage Revealed by Electron Cryomicroscopy2010

    • Author(s)
      Takayuki Kato, et al
    • Journal Title

      Structure

      Volume: 18 Pages: 719-724

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solution structure of the E. coli ribosome hibernation promoting factor HPF : Implications for the relationship between structure and function.2009

    • Author(s)
      Akiko Sato, Takumi Watanabe, Yasushi Maki, Masami Ueta, Hideji Yoshida, Yutaka Ito, Akira Wada, Masaki Mishima
    • Journal Title

      Biochemical and Biophysical Research Communications vol.389

      Pages: 580-585

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solution structure of the E.coli ribosome hibernation promoting factor HPF : Implications for the relationship between structure and function2009

    • Author(s)
      Akiko Sato, Takumi Watanabe, Yasushi Maki, Masami Ueta, Hideji Yoshida, Yutaka Ito, Akira Wada, Masaki Mishima
    • Journal Title

      Biochem.Biophys.Res.Commun. 389

      Pages: 580-585

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activities of E. coli ribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase2009

    • Author(s)
      H. Yoshida, M. Ueta, Y. Maki, A. Sakai, A. Wada
    • Journal Title

      Genes to Cells 14

      Pages: 271-280

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of HPF (Hibernation Promoting Factor) in Translational Activity in Escherichia coli.2008

    • Author(s)
      Masami Ueta, Ryosuke L, Ohniwa, Hideji Yoshida, Yasushi Maki, Chieko Wada, Akira Wada
    • Journal Title

      Journal of Biochemistry vol.143

      Pages: 425-433

    • NAID

      10024909025

    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸菌の生活環と栄養環境応答のトランスクリプトーム2011

    • Author(s)
      牧泰史, 等
    • Organizer
      第5回日本ゲノム微生物学会年会
    • Place of Presentation
      東北学院大学 土樋キャンパス(宮城県)(震災のため開催中止・要旨集配布により年会成立)
    • Year and Date
      2011-03-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌の栄養縣応答のトランスクリプトーム2010

    • Author(s)
      牧泰史, 等
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Transcriptome analysis of the Excherichia coli in the prolonged stationary phase and lag phase2010

    • Author(s)
      Yasushi Maki, et al
    • Organizer
      The 11^<th> Asian and Oceanian Conference on Transcription
    • Place of Presentation
      Hotel Bel Paraiso (Okinawa, Japan)
    • Year and Date
      2010-07-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌の栄養環境応答のトランスクリプトーム解析2010

    • Author(s)
      牧泰史, 道瀬ひとみ, 竹内力矢, 上田雅美, 和田明, 古池晶, 吉田秀司, 中東憲治, 森浩禎
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      九州大学医学部百年講堂(福岡)
    • Year and Date
      2010-03-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌の栄養環境応答のトランスクリプトーム2010

    • Author(s)
      牧泰史
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Related Report
      2010 Final Research Report
  • [Presentation] Transcriptome analysis of the Escherichia coli in the prolonged stationary phase and lag phase.2010

    • Author(s)
      牧泰史
    • Organizer
      The 11th Asian and Oceanian Conference on Transcription
    • Place of Presentation
      Hotel Bel Paraiso (Okinawa, Japan)
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸菌の栄養環境応答のトランスクリプトーム解析2010

    • Author(s)
      牧泰史
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      九州大学医学部(福岡県)
    • Related Report
      2010 Final Research Report
  • [Presentation] Structural analysis of 100S ribosome by cryoEM : tRNA binding2009

    • Author(s)
      吉田秀司, 加藤貴之, 牧泰史, 古池晶, 上田雅美, 境晶子, 和田明, 難波啓一
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] GraFix法により安定化した100SリボソームのcryoEMによる構造解析2009

    • Author(s)
      吉田秀司, 加藤貴之, 牧泰史, 古池晶, 上田雅美, 境晶子, 和田明, 難波啓一
    • Organizer
      第11回 RNAミーティング
    • Place of Presentation
      新潟市朱鷺メッセ(新潟)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌の生活環を通したトランスクリプトーム解析2009

    • Author(s)
      牧泰史, 道瀬ひとみ, 竹内力矢, 上田雅美, 和田明, 吉田秀司, 中東憲治, 森浩禎
    • Organizer
      第3回日本ゲノム微生物学会年会
    • Place of Presentation
      中央大学理工学部(後楽園キャンパス)
    • Related Report
      2008 Annual Research Report
  • [Presentation] E. coli YqjD expresses during the stationary phase and binds to ribosomes2008

    • Author(s)
      H. Yoshida, Y. Maki, M. Ueta, A. Sakai, A. Wada
    • Organizer
      RNA2008 Thirteenth annual meeting of the RNA society
    • Place of Presentation
      ベルリン(ドイツ)
    • Related Report
      2008 Annual Research Report
  • [Presentation] 内膜およびリボソームに結合する大腸菌蛋白質YqjDの機能解析2008

    • Author(s)
      吉田秀司, 牧泰史, 境晶子, 上田雅美, 和田明
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会)
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.osaka-med.ac.jp/~yhide/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.osaka-med.ac.jp/~yhide/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.osaka-med.ac.jp/~yhide/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.osaka-med.ac.jp/~yhide/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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