Distribution of condensins and chromosome architecture
| Project/Area Number |
20570009
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
ONO Takao The Institute of Physical and Chemical Research, 平野染色体ダイナミクス研究室, 専任研究員 (20291172)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | コンデンシンI / コンデンシンII / 染色体凝縮 / 姉妹染色分体 / DNA複製 / 複製ストレス / 人工染色体 / ピストン修飾 / G-バンド / 姉妹染色分体交換 / DNA損傷 / コヒーシン / コンデンシン / ヒストン修飾 / セントロメア / ヘテロクロマチン / キネトコア / 染色体バンド |
|---|
| Research Abstract |
Eukaryotic cells possess two different condensin complexes, known as condensin I and II. Both play essential yet distinct functions in the processes of mitotic chromosome assembly and segregation. Interestingly, condensin II tends to be enriched in G-band regions. It has long been known that G-band regions are replicated later in S phase and condense earlier in prophase. This set of information allows us to propose the working hypothesis that condensin II may play a role in linking DNA replication in S phase to chromosome condensation in M phase. Quantitative cell imaging analyses combined with immunostaining show that condensin II start to associate with chromosomes during S phase. Interestingly, in the presence of replication stress, the depletion of condensin II, but not of condensin I, induced a critical abnormality in chromosome in M phase. These results suggest that condensin II may play a crucial role in structural conversion of replicated chromosomes during S phase to facilitate chromosome condensation in M phase.
|
Report
(4 results)
Research Products
(25 results)
-
[Journal Article] A novel mutation in the C-terminal region of RUNX2/CBFA1 distal to the DNA-binding runt domain in a Japanese patient with cleidocranial dysplasia.2011
Author(s)
Kamamoto, M., Machida, J., Miyachi, H., Ono T., Nakayama, A., Shimozato, K., Tokita, Y
-
Journal Title
International Journal of Oral & Maxillofacial Surgery 40
Pages: 434-437
Related Report
Peer Reviewed
-
-
[Journal Article] Characterization of a de novo balanced t(4;20)(q33;ql2) translocation in a patient with mental retardation.2010
Author(s)
Yamada, K., Fukushi, D., Ono T., Kondo, Y., Kimura, R., Nomura, N., Kosaki, KJ., Yamada, Y., Mizuno, S., Wakamatsu, N
-
Journal Title
American Journal of Medical Genetics, Part A 152A
Pages: 3057-3067
Related Report
Peer Reviewed
-
-
[Journal Article] Searching for genes for cleft lip and/or palate based on breakpoint analysis of a balanced translocation t(9;17)(q32;q12).2009
Author(s)
Machida, J., Felix, TM., Murray, JC., Yoshiura, K., Tanemura, M., Kamamoto, M., Shimozato, K., Sonta, S., Ono T.
-
Journal Title
Cleft Palate-Craniofacial Journal 46
Pages: 532-540
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
