Estimation of the bacterial horizontal gene transfer frequency in environment from the transposition of mercury resistance transposon.

• Project/Area Number: 20570027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Kinki University
• Principal Investigator: MATSUI Kazuaki Kinki University, 理工学部, 講師 (40435532)
• Research Collaborator: ENDO Ginro 東北学院大学, 工学部, 教授 (80194033)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 遺伝子水平伝播 / 水銀耐性細菌 / 水銀耐性トランスポゾン / 遺伝子の分散 / 芽胞形成水銀耐性細菌 / TnMERI1 / 遺伝子の水平伝播 / 水銀耐性

Research Abstract

Horizontal gene transfer (HGT) is known to be an important mechanism for acquiring new genetic information in bacterial evolution. Mobile genetic materials, such as plasmid and transposon, contribute to HGT events among bacteria, while their transfer frequency and geological distribution have yet to be characterized well. This study demonstrated the worldwide distribution of TnMERI1 type transposons among bacteria. Further quantitative analysis showed the uneven distribution of TnMERI1 type transposons in soil environments. These results indicate the occurrence of bacterial HGT in natural environment.

Research Products

• [Journal Article] Organomercurials removal by heterogeneous merB genes harboring bacterial strains. (2010)
  • Author(s): Chien M.F., M.Narita, K.H.Lin, K.Matsui, C.C.Huang, G.Endo
  • Journal Title: Journal of Bioscience and Bioengineering 110 Pages: 94-98
  • NAID: 110007681451
  • Peer Reviewed
• [Journal Article] Quantification of cyprinid herpesvirus 3 in environmental water by using an external standard virus. (2010)
  • Author(s): Honjo M.N., T.Minamoto, K.Matsui, K.Uchii, H.Yamanaka, A.A.Suzuki, Y.Kohmatsu, T.Iida, Z.Kawabata
  • Journal Title: Applied and Environmental Microbiology 76 Pages: 161-168
  • Peer Reviewed
• [Journal Article] Organomercurials removal by heterogeneous merB genes harboring harterial strains. (2010)
  • Author(s): M.F.Chien, K.Matsui, 他4名
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 110 Pages: 94-98
  • Peer Reviewed
• [Journal Article] Quantification of Cyprinid Herpesvirus 3 in environmental water by using an external standard virus (2010)
  • Author(s): M.N.Honjo, T.Minamoto, K.Matsui, 他6名
  • Journal Title: Applied and Environmental Microbiology 76 Pages: 161-168
  • Peer Reviewed
• [Presentation] 水銀還元酵素遺伝子(merA)を指標にした土壌の水銀耐性細菌数評価の試み (2010)
  • Author(s): 吉浪賢史、成田勝、遠藤銀朗、松井一彰
  • Organizer: 第26回日本微生物生態学会(講演番号P-193)
  • Place of Presentation: つくば、つくば大学
  • Year and Date: 2010-11-25
• [Presentation] 水銀還元酵素遺伝子(merA)を指標とした土壌の水銀耐性細菌数評価の試み (2010)
  • Author(s): 松井一彰、吉浪賢史, 他2名
  • Organizer: 第26回日本微生物生態学会
  • Place of Presentation: 筑波大学
  • Year and Date: 2010-11-24
• [Presentation] Capacity and specificity of organomercurials detoxification by heterogeneous merB genes expressed in recombinant Escherichia coli (2008)
  • Author(s): 成田勝、簡梅芳、松井一彰、黄介辰、遠藤銀朗
  • Organizer: 第24回日本微生物生態学会(講演番号07-121)
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 2008-11-26
• [Presentation] 大腸菌における遺伝子水平伝播経路を指標にした淡水環境特性の評価 (2008)
  • Author(s): 松井一彰、本庄三恵、川端善一郎、内井喜美子
  • Organizer: 第24回日本微生物生態学会(講演番号04-068)
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 2008-11-26
• [Presentation] 環境水中に存在するコイヘルペスウィルスの定量 (2008)
  • Author(s): 本庄三恵、源利文、松井一彰、内井喜美子、山中裕樹、鈴木新、神松幸弘、飯田貴次、川端善一郎
  • Organizer: 日本陸水 学会第73回大会(1B18)
  • Place of Presentation: 札幌、北海道大学
  • Year and Date: 2008-10-11