Isolation and characterization of novel factors that regulate asymmetric cell division in plants

• Project/Area Number: 20570030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: FUJITA Tomomichi Hokkaido University, 大学院・理学研究院, 准教授 (50322631)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 植物 / 細胞分裂 / 細胞極性 / 発生・分化 / 遺伝子

Research Abstract

Since little is known about molecular mechanisms of asymmetric cell division in plants, we undertook to identify genes that are involved in the processes. The moss, Physcomitrella patens provides a good system for the study of asymmetric cell division. We identified more than 60 genes that impaired asymmetric cell division when overexpressed. We explored dynamics of the candidate's proteins during asymmetric cell division by introducing a yellow fluorescent protein as a tag into the c-terminus. We found proteins with unknown function, plant-specific transcription factors, chromatin remodeling factors, small G proteins related to asymmetric cell division. Moreover, putative cell wall proteins are responsible for a suppression of the asymmetric cell division.

Research Products

• [Journal Article] The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants. (2011)
  • Author(s): Banks J.A., Nishiyama T., Hasebe M.,Bowman J.L., Gribskov M., dePamphilis C., Albert V.A., Aono N., Aoyama T., Ambrose B.A., Ashton N.W., Axtell M.J., Barker E., Barker M.S., Bennetzen J.L., Bonawitz N.D., Chapple C., Cheng C., Correa L.G.G., Dacre M., DeBarry J., Dreyer I., Elias M., Engstrom E.M., Estelle M., Feng L., Finet C., Floyd S.K., Frommer W.B., Fujita T., (他73名)
  • Journal Title: Science in press
  • Peer Reviewed
• [Journal Article] Convergences and divergences in polarauxin transport and shoot development inland plant evolution. (2009)
  • Author(s): Fujita, T., Hasebe, M.
  • Journal Title: Plant Signaling & Behavior 4 Pages: 313-315
  • Peer Reviewed
• [Journal Article] Microtubules regulate dynamic organization of vacuoles in Physcomitrella patens. (2009)
  • Author(s): Oda, Y., Hirata, A., Sano, T., Fujita, T.,Hiwatashi, Y., Sato Y., Kadota, A., Hasebe, M., Hasezawa, S.
  • Journal Title: Plant and Cell Physiology 50 Pages: 855-868
  • NAID: 10027339794
  • Peer Reviewed
• [Journal Article] Convergences and divergences in polar auxin transport and shoot development in land plant evolution (2009)
  • Author(s): Fujita, T.
  • Journal Title: Plant Signaling & Behavivor 4 Pages: 313-315
• [Journal Article] Kinesins are indispensable for interdigitation of phragmoplastmicrotubules in the moss Physcomitrellapatens. (2008)
  • Author(s): Hiwatashi, Y., Obara, M., Sato, Y., Fujita, T., Murata, T., Hasebe, M.
  • Journal Title: Plant Cell 20 Pages: 3094-3106
  • Peer Reviewed
• [Journal Article] Expression and complementation analyses of a chloroplast-localized homolog of bacterialRecA in the moss Physcomitrella patens.Biosci. (2008)
  • Author(s): Inouye T., Odahara M., Fujita T., Hasebe M., Sekine Y.
  • Journal Title: Biotechnol. Biochem. 72 Pages: 1340-1347
  • Peer Reviewed
• [Journal Article] Expression and complementation analyses of a chloroplast-1 ocalized homolog of bacterial RecA in the moss Physcomit rella patens (2008)
  • Author(s): Inoue, T.
  • Journal Title: Biosci. Biotechnol. Biochem 72 Pages: 1340-1347
  • Peer Reviewed
• [Journal Article] Kinesins are indispensable for interdisitation of phragmopla st microtubules in the moss Physcomitrella patens (2008)
  • Author(s): Hiwatashi, Y.
  • Journal Title: Plant Cell 20 Pages: 3094-3106
  • Peer Reviewed
• [Presentation] アブシジン酸により誘導される細胞分裂と細胞分化に関わる新奇因子の同定と解析 (2011)
  • Author(s): 土屋祐弥
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学
• [Presentation] ヒメツリガネゴケの不等分裂幹細胞におけるCDKAの機能解析 (2011)
  • Author(s): 巻口勇馬
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学
• [Presentation] ヒメツリガネゴケの不等分裂幹細胞におけるCDKA ; 1の機能解析 (2010)
  • Author(s): 巻口勇馬
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] アブシジン酸による細胞分裂様式変更に関わる新奇因子の単離と解析 (2010)
  • Author(s): 土屋祐弥
  • Organizer: 日本植物学会
  • Place of Presentation: 中部大学
• [Presentation] Switching between asymmetric cell division and symmetric cell division in the moss, Physcomitrella patens. (2010)
  • Author(s): 藤田知道
  • Organizer: MOSS2010
  • Place of Presentation: Sapporo
• [Presentation] ヒメツリガネゴケ原糸体を用いた細胞レベルでの原形質連絡制御の解析 (2010)
  • Author(s): 北川宗典
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] ヒメツリガネゴケにおけるABA による不等分裂から等分裂への切り替えの解析 (2010)
  • Author(s): 中村康平
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] Switching between asymmetric cell division and symmetric cell division in the moss, Physcomitrella patens (2010)
  • Author(s): Tomomichi Fujita
  • Organizer: MOSS2010
  • Place of Presentation: Sapporo
• [Presentation] ヒメツリガネゴケの不等分裂幹細胞におけるCDKA;1の機能解析 (2010)
  • Author(s): 巻口勇馬
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ヒメツリガネゴケにおけるABAによる不等分裂から等分裂への切り替えの解析 (2010)
  • Author(s): 中村康平
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本
• [Presentation] ヒメツリガネゴケにおいて不等分裂制御への関与が推測されるGRASファミリー転写因子の機能解析 (2009)
  • Author(s): 一力綾子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] ヒメツリガネゴケにおけるABA による不等分裂から等分裂への切り替えの解析 (2009)
  • Author(s): 中村康平
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Functional analyses of genesresponsible for asymmetric cell division inPhyscomitrella patens (2009)
  • Author(s): 藤田知道
  • Organizer: MOSS2009
  • Place of Presentation: St.Louis, MO, USA
• [Presentation] 不等分裂モデルであるヒメツリガネゴケ頂端幹細胞に局在するタンパク質群の同定および機能解析 (2009)
  • Author(s): 藤田知道
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
• [Presentation] 原形質連絡による細胞間情報伝達制御と細胞の分化状態との関係性を探る (2009)
  • Author(s): 北川宗典
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋大学
• [Presentation] Functional analyses of genes responsible for asymmetric cell division in Physcomitrella patens (2009)
  • Author(s): 藤田知道
  • Organizer: MOSS2009
  • Place of Presentation: St.Louis, MO
• [Presentation] ヒメツリガネゴケにおけるABAによる不等分裂から等分裂への切り替えの解析 (2009)
  • Author(s): 中村康平
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 植物幹細胞に蓄積するタンパク質群のタイムラプス解析 (2008)
  • Author(s): 藤田知道
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ヒメツリガネゴケにおける不等分裂に関わる因子の相互作用 (2008)
  • Author(s): 一力綾子
  • Organizer: 日本植物学会
  • Place of Presentation: 高知大
• [Presentation] Spatio-temporal accumulation of proteins during asymmetric stem cell division in Physcomitrella patens (2008)
  • Author(s): 藤田知道
  • Organizer: MOSS2008
  • Place of Presentation: Tampere, Finland
• [Presentation] Spatio-temporal accmulation of proteins during asymmetric stem cell division in Physcomitrella patens (2008)
  • Author(s): Fujita, T.
  • Organizer: MOSS2008
  • Place of Presentation: Tampere, Finland
• [Presentation] ヒメツリガネゴケにおける不等分裂に関わる因子の相互作用 (2008)
  • Author(s): 一力綾子
  • Organizer: 日本植物学会
  • Place of Presentation: 高知大学
• [Book] 植物の百科事典 (2009)
  • Author(s): 藤田知道
  • Publisher: 朝倉書店
• [Book] 植物の百科事典 (2009)
  • Author(s): 石井龍一
  • Publisher: 朝倉書店
• [Remarks] ホームページ等
  • URL: http://www.sci.hokudai.ac.jp/~tfujita/Fujita/welcome.html
• [Remarks]
  • URL: http://www.sci.hokudai.acjp/~tfujita/Fujita/welcome.html
• [Remarks]
  • URL: http://www.sci.hokudai.ac.jp/~tfujita/Fujita/welcome.html