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Analysis of the different chloroplast biogenesis in cotyledon and leaf

Research Project

Project/Area Number 20570032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionHiroshima University

Principal Investigator

HIROSHI Shimada  Hiroshima University, 大学院・理学研究科, 准教授 (80301175)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords色素体 / 葉緑体 / エチオプラスト / 光合成 / シロイヌナズナ / Protein disulfide isomerase / 子葉 / 本葉 / 植物
Research Abstract

Arabidopsis chloroplast biogenesis factor CYO2/At3g47650 is a cotyledon-specific chloroplast biogenesis factor CYO1 homolog. The cyo2 mutant showed pale-green leaves, and the maximum quantum yield of photosystem II in the mutant was much decreased. The expression of many photosynthetic genes was significantly decreased in the mutant. CYO1 expressed in only seedlings grown under light conditions, however, CYO2 expressed in not only all green tissues but also seedlings grown under dark conditions. It was also observed that the difference of characteristics of CYO1 and CYO2 reductase activity of protein disulfide isomerase.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (29 results)

All 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (17 results) Remarks (1 results)

  • [Journal Article] Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells2010

    • Author(s)
      Shimada, H., Obayashi, T., Takahashi, N., Matusi M., Sakamoto A.
    • Journal Title

      Plant Methods 6

      Pages: 1-9

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis2010

    • Author(s)
      Watanabe, S., Nakagawa, A., Izumi, S., Shimada, H., Sakamoto A.
    • Journal Title

      FEBS Lett. 584

      Pages: 1181-1186

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast biogenesis during the early stage of leaf development in rice2010

    • Author(s)
      Kusumi, K., Chono, Y., Shimada, H., Gotoh, E., Tsuyama, M., Iba, K.
    • Journal Title

      Plant Biotech. 27

      Pages: 85-90

    • NAID

      10026411513

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice2010

    • Author(s)
      Kusumi, L, Hirotsuka, S., Shimada, H., Chono, Y., Iba, K.
    • Journal Title

      J.Plant Res. 123

      Pages: 617-622

    • NAID

      10027220182

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Normalization using ploidy and genomic DNA copy tiumber allows absolute quantification of transcripts, protiens and metabolites in cells.2010

    • Author(s)
      Hiroshi Shimada, et al.
    • Journal Title

      Plant Methods

      Volume: 6:29 Pages: 1-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerancein Arabidopsis.2010

    • Author(s)
      Shunsuke Watanabe, et al.
    • Journal Title

      FEBS Lett.

      Volume: 584 Pages: 1181-1186

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast biogenesis during the early stage of leaf development in rice.2010

    • Author(s)
      Kensuke Kusumi, et al.
    • Journal Title

      Plant Biotechnology

      Volume: 27 Pages: 85-90

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice.2010

    • Author(s)
      Kensuke Kusumi, et al.
    • Journal Title

      J.Plant Res.

      Volume: 123 Pages: 617-622

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis2010

    • Author(s)
      Watanabe, S., et al.
    • Journal Title

      FEBS Lett. 584

      Pages: 1181-1186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast biogenesis during the early stage of leaf development in rice2010

    • Author(s)
      Kusumi, K., et al.
    • Journal Title

      Plant Biotechnology (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice2010

    • Author(s)
      Kusumi K., et al.
    • Journal Title

      J.Plant Res. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] ゲノムコピー数と倍数性に基づいたABCD (Analysis Based on Copy number of genomic DNA)法による細胞1個当たりのmRNA数,タンパク質数,代謝産物数の絶対定量法の開発2011

    • Author(s)
      島田裕士, 大林武, 高橋直紀, 松井南, 坂本敦
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノムコピー数と倍数性に基づいたABCD (Analysis Based on Copy number of genomic DNA)法による細胞1個当たりのmRNA数、タンパク質数、代謝産物の絶対定量法の開発2011

    • Author(s)
      島田裕士, その他
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナのストレス適応におけるプリン分解代謝の生理的役割2011

    • Author(s)
      渡邉俊介, その他
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 緑色組織におけるシロイヌナズナ葉緑体形成因子CYO2の解析2011

    • Author(s)
      室屋誠人, 伊東千賀子, 村中厚子, 水谷春香, 坂本敦, 島田裕士
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナのストレス応答におけるプリン分解代謝の生理的役割2011

    • Author(s)
      渡邉俊介, 杉本高文, 前田智美, 島田裕士, 坂本敦
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 緑色組織におけるシロイヌナズナ葉緑体形成因子CYO2の解析2011

    • Author(s)
      室谷誠人, その他
    • Organizer
      第52回日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] ABCD, Analysis Based on Copy number of genomic DNA, method reveals the number of transcripts, proteins and metabolites in a cell of Arabidopsis.2010

    • Author(s)
      Shimada H., Obayash, T., Sakamoto A.
    • Organizer
      21^<st> International conference on Arabidopsis research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Physiological contribution o fpurine metabolism in drought acclimatization of Arabidopsis.2010

    • Author(s)
      渡邉俊介, 中川彩美, 島田裕士, 坂本敦
    • Organizer
      21^<st> International conference on Arabidopsis research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-07
    • Related Report
      2010 Final Research Report
  • [Presentation] ABCD, Analysis Based on Copy number of genomic DNA, method reveals the number of transcripts, proteins and metabolites in a cell of Arabidopsis.2010

    • Author(s)
      Hiroshi Shimada, et al.
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Physiological contribution of purine metabolism in drought acclimatization of Arabidopsis2010

    • Author(s)
      Shunsuke Wataanbe, et al.
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-06-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞1個当たりのmRNA数,タンパク質数,代謝産物数の簡易同定方法の開発2010

    • Author(s)
      島田裕士, 大林武, 坂本敦
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナの乾燥ストレス適応におけるプリン代謝の生理機能の解析2010

    • Author(s)
      渡邉俊介, 中川彩美, 島田裕士, 坂本敦
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 細胞1個当たりのmRNA数、タンパク質数、代謝産物数の簡易同手法の開発2010

    • Author(s)
      島田裕士, 他2名
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナ子葉特異的葉緑体形成因子CYO1の酵素学的解析2010

    • Author(s)
      村中厚子, 坂本敦, 島田裕士
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-19
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 乾燥ストレス適応におけるシロイヌナズナ・キサンチン脱水素酵素の機能解析2009

    • Author(s)
      渡邊俊介, 中川彩美, 島田裕士, 坂本敦
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] シュウ酸酸化活性を有するツツジGLPを過剰発現するタバコ培養細胞の重金属感受性2009

    • Author(s)
      西村崇, 宮木洋一, 高橋美佐, 森川弘道, 泉俊輔, 島田裕士, 坂本敦
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] シュウ酸酸化活性を有するツヅジGLPを過剰発現するタバコ培養細胞の重金属感受性2009

    • Author(s)
      西村崇, 宮木洋一, 高橋美佐, 森川弘道, 泉俊輔, 島田裕士, 坂本敦
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-22
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/mpb/index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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