Posttranscriptional regulation of plastid gene expression in Physcomitrella patens.

• Project/Area Number: 20570033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: SUGITA Mamoru Nagoya University, 遺伝子実験施設, 教授 (70154474)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 葉緑体 / ミトコンドリア / 転写後制御 / RNA編集 / PPRタンパク質 / ヒメツリガネゴケ / 遺伝子破壊株 / DYWドメイン / 遺伝子ノックアウト株 / 遺伝子発現 / シロイヌナズナ

Research Abstract

Chloroplasts possess their own DNA and approximately 100 genes. Chloroplast function and gene expression are cooperatively controlled by the chloroplast and nuclear genomes. Posttranscriptional regulation is an important step in the control of chloroplast gene expression. In this study, we identified novel nuclear-encoded pentatricopeptide repeat proteins involved in RNA splicing, RNA cleavage, and RNA splicing in chloroplasts.

Research Products

• [Journal Article] The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants. (2011)
  • Author(s): Banks, J.A., Nishiyama, T., Hasebe, M., Sugita, M.
  • Journal Title: Science 332(6032)
• [Journal Article] DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum. (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., Sasaki, N.
  • Journal Title: Mitochondrion in press
• [Journal Article] DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum. (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., Sasaki, N.
  • Journal Title: Mitochondrion
  • Peer Reviewed
• [Journal Article] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts. (2010)
  • Author(s): Ohtani, S., Ichinose, M., Tasaki, E., Aoki, Y., Komura, Y., Sugita, M.
  • Journal Title: Plant and Cell Physiology 52(11) Pages: 1942-1949
  • NAID: 10028056052
• [Journal Article] The moss Physcomitrella patens, a model plant for the study of RNA editing in plant organelles. (2010)
  • Author(s): Tasaki, E., Sugita, M.
  • Journal Title: Plant Signaling & Behavior 5(6) Pages: 727-729
• [Journal Article] The moss pentatricopeptide repeat protein with a DYW domain is responsible for RNA editing of mitochondrial ccmFc transcript. (2010)
  • Author(s): Tasaki, E., Hattori, M., Sugita, M.
  • Journal Title: The Plant Journal 62(4) Pages: 560-570
• [Journal Article] The moss pentatricopeptide repeat protein with a DYW domain is responsible for RNA editing of mitochondrial ccmFc transcript. (2010)
  • Author(s): Tasaki, E., Hattori, M., Sugita. M.
  • Journal Title: Pant Journal Volume: 62 Pages: 560-570
  • Peer Reviewed
• [Journal Article] The moss Physcomitrella patens, a model plant for the study of RNA editing in plant organelles. (2010)
  • Author(s): Tasaki, E., Sugita, M.
  • Journal Title: Plant Signaling & Behavior Volume: 5 Pages: 727-729
  • Peer Reviewed
• [Journal Article] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts. (2010)
  • Author(s): Ohtani, S., Ichinose, M., Tasaki, E., Aoki, Y., Komura, Y., Sugita, M.
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 1942-1949
  • NAID: 10028056052
  • Peer Reviewed
• [Journal Article] The moss pentatricopeptide repeat protein with a DYW domain is responsible for RNA editing of mitochondrial ccmFc transcript. (2010)
  • Author(s): Tasaki, E., Hattori, M., Sugita, M.
  • Journal Title: The Plant journal 62(In press)
  • Peer Reviewed
• [Journal Article] A moss pentatricopeptide repeat protein binds to the 3'-end of plastid clpP pre-mRNA and assists with the mRNA maturation. (2009)
  • Author(s): Hattori, M., Sugita, M.
  • Journal Title: FEBS Journal 276(20) Pages: 5860-5869
• [Journal Article] Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus. (2009)
  • Author(s): Ito, H., Mutsuda, M., Murayama, Y., Tomita, J., Hosokawa, N., Terauchi, K., Sugita, C., Sugita, M., Kondo, T., Iwasaki, H.
  • Journal Title: Proc.Natl Acad.Sci.USA 106(33) Pages: 14168-14173
• [Journal Article] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. (2009)
  • Author(s): Okuda, K.Chateigner-Boutin, A.L., Nakamura, T., Delannoy, E., Sugita, M., Myouga, F., Motohashi, R., Shinozaki, K., Small, I.Shikanai, T.
  • Journal Title: The Plant Cell 21 Pages: 146-156
• [Journal Article] A moss pentatricopeptide repeat protein binds to the 3'-end of plastid clpP pre-mRNA and assists with the mRNA maturation. (2009)
  • Author(s): Hattori, M., Sugita, M.
  • Journal Title: FEBS Journal 276 Pages: 5860-5869
  • Peer Reviewed
• [Journal Article] Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus. (2009)
  • Author(s): Ito, H., Mutsuda, M., Murayama, Y., Tomita, J., Hosokawa, N., Terauchi, K., Sugita, C., Sugitg a_M., Kondo, T., Iwasaki, H.
  • Journal Title: Proc. Nati. Acad. Sci. USA 106 Pages: 14168-14173
  • Peer Reviewed
• [Journal Article] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. (2009)
  • Author(s): Okuda, K., Chateigner-Boutin, A. L., Nakamura, T., Delannoy, E., Sugita, M., Myouga, F., Motohashi, R., Shinozaki, K., Small, I., Shikanai, T.
  • Journal Title: The Plant Cell 21 Pages: 146-156
  • Peer Reviewed
• [Journal Article] 1章 植物・藻類・細菌の材料の入手と栽培・培養5. ヒメツリガネゴケ (2009)
  • Author(s): 青木摂之, 杉田護
  • Journal Title: 低温科学 67 Pages: 31-33
  • Peer Reviewed
• [Journal Article] 5章形質転換4. ヒメツリガネゴケ (2009)
  • Author(s): 杉田護, 田崎瑛示, 香村吉洋
  • Journal Title: 低温科学 67 Pages: 607-613
  • Peer Reviewed
• [Journal Article] A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribonuclease activity. (2008)
  • Author(s): Nakamura, T., Sugita, M.
  • Journal Title: FEBS Letters 582(30) Pages: 4163-4168
• [Journal Article] Characteristics and prediction of RNA editing sites in the transcripts of a moss Takakia lepidozioides chloroplast. (2008)
  • Author(s): Yura, K., Miyata, Y., Arikawa, T., Higuchi, M., Sugita, M.
  • Journal Title: DNA Research 15(5) Pages: 309-321
• [Journal Article] Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice. (2008)
  • Author(s): Kazama, T., Nakamura, T., Watanabe, M.,Sugita, M., Toriyama, K.
  • Journal Title: The Plant Journal 55 Pages: 619-628
• [Journal Article] On the expansion of the pentatricopeptide repeat gene family in plants. (2008)
  • Author(s): O'Toole, N., Hattori, M., Andres, C., Iida, K., Claire Lurin, C., Schmitz-Linneweber, C., Sugita, M., Small, I.
  • Journal Title: Molecular Biology and Evolution 25(6) Pages: 1120-1128
• [Journal Article] RNA editing in the anticodon of tRNALeu (CAA) occurs before group I intron splicing in plastids of a moss Takakia lepidozioides S. Hatt. & Inoue. (2008)
  • Author(s): Miyata, Y., Sugita, C., Maruyama, K., Sugita, M.
  • Journal Title: Plant Biology 10 Pages: 250-255
• [Journal Article] A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribomuclease activitv. (2008)
  • Author(s): Nakamura, T., Sugita. M.
  • Journal Title: FEBS Letters 582 Pages: 4163-4168
  • Peer Reviewed
• [Journal Article] Characteristics and prediction of RNA editing sites in the transcripts of amoss Takakia lepidozioides chloroplast. (2008)
  • Author(s): Yura, K., Miyata, Y., Arikawa, T., Higuchi, M., Sugita. M.
  • Journal Title: DNA Research 15 Pages: 309-321
  • Peer Reviewed
• [Journal Article] Suppression mechanism of mitochondrial ORF79 accumulation by Rflprotein in BT-type cytoplasmic male sterile rice. (2008)
  • Author(s): Kazama, T., Nakamura, T., Watanabe, M., Sugjta, M., Toriyama, K.
  • Journal Title: The Plant Journal 55 Pages: 619-628
  • Peer Reviewed
• [Journal Article] On the expansion of the pentatricopeptide repeat gene family in plants. (2008)
  • Author(s): O'Toole, N., Hattori, M., Andres, C., Iida, K., Claire Lurin, C., Schmittz-Liimeweber, C., Sueita. M., Small, I.
  • Journal Title: Molecular Biology and Evolution 25 Pages: 1120-1128
  • Peer Reviewed
• [Journal Article] RNA editing in the anticodon of tRNA^<Leu> (CAA) occurs before group I intron splicing in plastids of a moss Takakia lepidozioides S. Hatt. & Inoue. (2008)
  • Author(s): Miyata, Y., Sugita, C., Maruyama, K., Sueita. M.
  • Journal Title: Plant Biology 10 Pages: 250-255
  • Peer Reviewed
• [Presentation] ヒメツリガネゴケのCRL遺伝子ファミリーの機能解析 (2011)
  • Author(s): 杉田千恵子、加藤大和、鶴見尚子、吉岡泰、町田泰則、杉田護
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] ヒメツリガネゴケPPR-DYWタンパク質はミトコンドリアRNAの編集とスプライシングに働く (2011)
  • Author(s): 一瀬瑞穂、大谷祥太郎、内田雅人、杉田護
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] Involvement of pentatricopeptide repeat proteins in regulation of plantorganelle gene expression. (2011)
  • Author(s): Mamoru Sugita
  • Organizer: 2011 Switzerland-Japan Workshop, Adaptation of the plastids to various environmental conditions : Evolution, acclimination and regulation.
  • Place of Presentation: Eurotel Villars-sur-Ollon、Switzerland
  • Year and Date: 2011-01-11
• [Presentation] DYWドメインを持つPPRタンパク質はミトコンドリアmRNAの蓄積レベルを制御する(2pG03) (2010)
  • Author(s): 一瀬瑞穂、田崎瑛示、杉田護
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2010-09-10
• [Presentation] ヒメツリガネゴケのミトコンドリアRNA編集因子の同定 (2010)
  • Author(s): 大谷祥太郎、田崎瑛示、香村吉洋、青木良晃、杉田護
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2010-09-10
• [Presentation] DYWドメインを持つPPRタンパク質はミトコンドリアmRNAの蓄積レベルを制御する (2010)
  • Author(s): 一瀬瑞穂、田崎瑛示、杉田護
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2010-09-10
• [Presentation] ヒメツリガネゴケCRUMPLED LEAF相同遺伝子破壊株の解析 (2010)
  • Author(s): 鶴見尚子、加藤大和、飯田由佳里、吉岡泰、杉田千恵子、杉田護
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2010-09-09
• [Presentation] Identification of RNA editing factors in the moss Physcomitrella patens (2010)
  • Author(s): Ohtani, S., Tasaki, E., Aoki, Y., Komura, Y., Sugita, C., Sugita, M.
  • Organizer: International Symposium on Biodiversity Sciences- Genome, Evolution and Environment
  • Place of Presentation: Hotel Rubura Ohzan, Nagoya.
  • Year and Date: 2010-08-02
• [Presentation] Physcomitrella patens, a model plant for studying the molecular basis of site-specific RNA editing. (2010)
  • Author(s): Sugita, M., Ohtani, S., Ichinose, M., Uchida, M., Sugita, M.
  • Organizer: MOSS2010, the annual international conference for experimental moss research
  • Place of Presentation: Lake Shikotsu, Hokkaido
  • Year and Date: 2010-07-24
• [Presentation] An analysis of CRUMPLED LEAF homologous genes of Physcomitrella patens by gene disruption (2010)
  • Author(s): Yoshioka, Y., Tsurumi, N., Kato, Y., Iida, Y., Machida, Y., Sugita, C., Sugita, M.
  • Organizer: MOSS2010, the annual international conference for experimental moss research
  • Place of Presentation: Lake Shikotsu, Hokkaido
  • Year and Date: 2010-07-24
• [Presentation] 人工マイクロRNAによるヒメツリガネゴケの遺伝子発現抑制系の開発 (2010)
  • Author(s): 杉田千恵子, 中山綾子, 鶴見尚子, 香村吉洋, 杉田護
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-21
• [Presentation] ヒメツリガネゴケのPPRタンパク質は質は部位特異的なRNA編集やRNAの蓄積レベルの制御に働く(3pH02) (2010)
  • Author(s): 田崎瑛示、香村吉洋、青木良晃、大谷祥太郎、永島はるか、杉田護
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] ヒメツリガネゴケのPPRタンパク質は部位特異的なRNA編集やRNAの蓄積レベルの制御に働く (2010)
  • Author(s): 田崎瑛示、香村吉洋、青木良晃、大谷祥太郎、永島はるか、杉田護
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] Physcomitrella patens, a model plant for studying the molecular basis of site-specific RNA editing (T30). (2010)
  • Author(s): Mamoru Sugita, Shotaro Ohtani, Mizuho Ichinose, Masato Uchida, Chieko Sugita
  • Organizer: Oral presented on July 24.MOSS2010, the annual international conference for experimental moss research
  • Place of Presentation: in Sapporo and its vicinity, Lake Shikotsu Resort,Hokkaido, Japan.
• [Presentation] RNA結合性pentatricopeptide repeat(PPR)タンパク質ファミリーの網羅的解析(4P-301) (2009)
  • Author(s): 杉田護、田崎瑛示、香村吉洋、青木良晃、井手瑞樹、服部満
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] RNA結合性pentatricopeptide repeat(PPR)タンパク質ラァミリーの網羅的解析 (2009)
  • Author(s): 杉坦護、田崎瑛示、香村吉洋、青木良晃、井手瑞樹、服部満
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2009-12-12
• [Presentation] ヒメツリガネゴケのPpPPR_63遺伝子を破壊すると原糸体の分枝形成が異常になる (2009)
  • Author(s): 香村吉洋, 佐藤浩之, 杉田護
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-24
• [Presentation] ミトコンドリアめRNA編集に働くDYWドメインをもつPPRタンパク質 (2009)
  • Author(s): 田崎瑛示, 青木良晃, 杉田護
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] ヒメツリガネゴケのPPRタンパク質のDYWモチーフはリボヌクレアーゼ活性を持つ (2009)
  • Author(s): 井手瑞樹, 田崎瑛示, 杉田千恵子, 杉田護
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-22
• [Presentation] ヒメツリガネゴケのPpCRL遺伝子ファミリーの機能解析 (2009)
  • Author(s): 鶴見尚子, 飯田由佳里, 杉田千恵子, 杉田護
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-21
• [Presentation] Helical repeat proteinのRNA配列特異性と生体プローブへの応用(1P-0881) (2008)
  • Author(s): 服部満、杉田護、小澤岳昌
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] Roles of pentatricopeptide repeat proteins in regulation of plastid gene expression. (2008)
  • Author(s): Mamoru Sugita, Mitsuru Hattori, Takahiro Nakamura, Eiji Tasaki
  • Organizer: Japan-Swiss Workshop on Photosynthetic Adaptation and Chloroplast Dynamics
  • Place of Presentation: 奈良市
  • Year and Date: 2008-10-07
• [Presentation] 陸上植物の葉緑体RNA編集の進化 (2008)
  • Author(s): 宮田有希、杉田護
  • Organizer: 日本植物学会第72回大会(高知)
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-25
• [Presentation] 陸上植物の葉緑体RNA編集の進化 (2008)
  • Author(s): 宮田有希, 杉田護
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-25
• [Presentation] 葉緑体に局在するペンタトリコペプチドリピート蛋白質ファミリーの機能解析 (2008)
  • Author(s): 杉田護, 田崎英示, 香村吉洋, 井手瑞樹
  • Organizer: 第8回日本光合成研究会公開シンポジウム
  • Place of Presentation: 名古屋大学
• [Presentation] ナンジヤモンジヤゴケ葉緑体のRNAエデイテイング部位の同定と予測 (2008)
  • Author(s): 由良敬, 宮田有希, 有川智己, 樋口正信, 杉田護
  • Organizer: 日本生物物理学会第46年会
  • Place of Presentation: 福岡国際会議場
• [Book] Chapter 8.Chloroplasts.In The Moss Physcomitrella patens(Celia Knight, Pierre Francois-Perround and David Cove, eds.)(Annual Plant Reviews 36) (2009)
  • Author(s): Sugita, M., Aoki, S.
  • Publisher: Blackwell Publishing
• [Book] 5章形質転換(4.ヒメツリガネゴケ、低温科学67巻) (2009)
  • Author(s): 杉田護、田崎英示、香村吉洋
  • Publisher: 北海道大学低温科学研究所
• [Book] Annual Plant Reviews, Volume 36, The Moss Physcomitrella patens(Celia Knight, Pierre Francois-Perround and David Cove, eds) (2009)
  • Author(s): Sugita, M., Aoki, S.(分担執筆)
  • Total Pages: 350
  • Publisher: Blackwell Publishing Ltd
• [Book] 植物ゲノム科学辞典(総編集駒嶺穆) (2009)
  • Author(s): 杉田護(分担執筆)
  • Total Pages: 406
  • Publisher: 朝倉書店
• [Book] 分子細胞生物学辞典(第2版) (2008)
  • Author(s): 杉田護(分担執筆)
  • Total Pages: 406
  • Publisher: 東京化学同人
• [Remarks] ホームページ等
  • URL: http://www.gene.nagoya-u.ac.jp/?sugita-g/
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.ip/~sugita-g/seika-4.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.ip/~sugita-g/seika-5.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/ronbun.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/seika-1.html
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/seika-2.miml
• [Remarks]
  • URL: http://www.gene.nagoya-u.ac.jp/~sugita-g/seika-3.html