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Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

Research Project

Project/Area Number 20570034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

SAZUKA Takashi  Nagoya University, 生物機能開発利用研究センター, 准教授 (70362291)

Co-Investigator(Renkei-kenkyūsha) KITANO Hidemi  名古屋大学, 生物機能開発利用研究センター, 教授 (50144184)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsイネ / 胚発生 / 器官形成 / オーキシン / マイクロRNA / 植物ホルモン / 生長生理 / 国際情報交換
Research Abstract

The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (9 results)

All 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Remarks (1 results)

  • [Journal Article] Mapping and characterization of the rice gene2010

    • Author(s)
      Imamura, K., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
    • Journal Title

      CLUB-SHAPED EMBRYO3, CLE3.Rice Gen.Newslett. 25

      Pages: 27-29

    • Related Report
      2010 Final Research Report
  • [Journal Article] Characterization and mapping of the CROWN ROOTLESS2 gene2010

    • Author(s)
      Yamamoto, Y., Inukai, Y., Kitano, H., Sazuka, T., Matsuoka, M.
    • Journal Title

      CRL2, in rice.Rice Gen.Newslett. 25

      Pages: 25-26

    • Related Report
      2010 Final Research Report
  • [Journal Article] Characterization and mapping of the CROWN ROOTLESS2 gene, CRL2, in rice.2010

    • Author(s)
      Yamamoto, Y., et al.
    • Journal Title

      Rice Gen.Newslet.

      Volume: 25 Pages: 25-26

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mappring and characterization of the rice gene, CLUB-SHAPED EMBRY03, CLE3.2010

    • Author(s)
      Imamura, K., et al.
    • Journal Title

      Rice Gen.Newslet.

      Volume: 25 Pages: 27-29

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.2009

    • Author(s)
      Sazuka, T., Kamiya, N., Nishimura, T., Ohmae, K., Sato, Y., Imamura, K., Nagato, Y., Koshiba, T., Nagamura, Y., Ashikari, M., Kitano, H., Matsuoka, M.
    • Journal Title

      Plant J. 60

      Pages: 227-241

    • Related Report
      2010 Final Research Report
  • [Journal Article] A rice trptophan deficient dwarfmutant, tddl, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos.2009

    • Author(s)
      Sazuka T., et al.
    • Journal Title

      Plant J. 60

      Pages: 227-241

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping and Analysis of the CLUB-SHAPED EMBRYO1, CLE1.2008

    • Author(s)
      Imamura, K., Imai, H., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
    • Journal Title

      Rice Gen.Newslett. 4

      Pages: 22-24

    • Related Report
      2010 Final Research Report
  • [Journal Article] Mapping and Analysis of the CLUB-SHAPED EMBRYOl, CLEl.2008

    • Author(s)
      Imamura, K., Imai, H., Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T.
    • Journal Title

      Rice Gen. Newslet. 24

      Pages: 22-24

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Remarks] ホームページ等

    • URL

      http://www.agr.nagoya-u.ac.jp/~naikan/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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