Molecular mechanism of cyanobacterial circadian clock using the reconstituted in-vitro oscillator.
| Project/Area Number |
20570035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
TERAUCHI Kazuki Ritsumeikan University, 生命科学部, 准教授 (70444370)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Yuichi 名古屋大学, 生命農学研究科, 准教授 (80222264)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | シアノバクテリア / 生物時計 / 概日時計 / 光 |
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| Research Abstract |
The circadian clock can be reconstituted in vitro by the KaiA, KaiB, and KaiC proteins in the presence of ATP. In this study, LdpA presumed to be a redox sensor in cyanobacteria was analyzed biochemically. The structure of the molecule was changed by a redox state. Moreover, it was found that pH of the KaiABC reconstitution system have an effect on the length of the cycle. The two factors, pH in the cell and LdpA, could get involved in the change of the period length the cycle by light.
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Report
(4 results)
Research Products
(32 results)
