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MOLECULAR MECHANISMS UNDERLYING THE REGULATION OF NUCLEAR STRUCTURE IN PLANTS

Research Project

Project/Area Number 20570036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyoto University

Principal Investigator

TAMURA Kentaro  Kyoto University, 大学院・理学研究科, 助教 (40378609)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords細胞核.シロイヌナズナ / GFP / プロテオミクス / 突然変異体 / 細胞核 / シロイヌナズナ
Research Abstract

The nuclear pore complex (NPC) is important multiprotein complex that mediate nucleus-cytoplasm trafficking in eukaryotic cells. To identify the components of NPC in Arabidopsis, we employed interactive proteomic approach. In his analysis, we successfully identified 30 nucleoproins, 22 of which were not annotated previously. To understand how plants build their nuclei, we isolated several lines of mutant that has defect in nuclear morphology.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009 2008 Other

All Journal Article (22 results) (of which Peer Reviewed: 21 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Involvement of the nuclear pore complex in morphology of the plant nucleus2011

    • Author(s)
      Kentaro Tamura, I.Hara-Nishimura
    • Journal Title

      Nucleus in press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Identification and characterization of nuclear pore complex components in Arabidopsis thaliana.2010

    • Author(s)
      Kentaro Tamura, Y.Fukao, M.Iwamoto, T.Haraguchi, I.Hara-Nishimura
    • Journal Title

      Plant Cell 12

      Pages: 4084-4097

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] MAG4/Atp115 is a golgi-localized tethering factor that mediates efficient anterograde transport in Arabidopsis.2010

    • Author(s)
      H.Takahashi, Kentaro Tamura, J.Takagi, Y.Koumoto, I.Hara-Nishimura, T.Shimada
    • Journal Title

      Plant Cell Physiol. 51

      Pages: 1777-1787

    • NAID

      10027353695

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway2010

    • Author(s)
      L.Marti, G.Stefano, Kentaro Tamura, C.Hawes, L.Renna, M.A.Held, F.Brandizzi
    • Journal Title

      Plant J. 63

      Pages: 901-913

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells.2010

    • Author(s)
      H.Ueda, E.Yokota, N.Kutsuna, T.Shimada, Kentaro Tamura, T.Shimmen, S.Hasezawa, V.V Dolja, I.Hara-Nishimura
    • Journal Title

      Proc Natl Acad Sci USA. 107

      Pages: 6894-6899

    • NAID

      120002511346

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Characterization of Nuclear Pore Complex Components in Arabidopsis thaliana2010

    • Author(s)
      Kentaro Tamura, et al.
    • Journal Title

      The Plant Cell

      Volume: 12 Pages: 4084-4097

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MAG4/Atp 115 is a golgi-localized tethering factor that mediates efficient anterograde transport in Arabidopsis.2010

    • Author(s)
      Hideyuki Takahashi, et al.
    • Journal Title

      Plant & Cell Physiology

      Volume: 51 Pages: 1777-1787

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway2010

    • Author(s)
      Marti Stefano, et al.
    • Journal Title

      Plant Journal

      Volume: 6 Pages: 901-913

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells2010

    • Author(s)
      Haruko Ueda, et al.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] *these authors equally contributed to the work. A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatus2009

    • Author(s)
      C.Faso*, Y.Chen*, Kentaro Tamura*, M.Held, S.Zemelis, L.Marti, R.Saravanan, E.Hummel, L.Kung, E.Miller, C.Hawes, F.Brandizzi
    • Journal Title

      Plant Cell 21

      Pages: 3655-3671

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ERMO1/GNL1 and ERMO2/SEC24a are required for maintenance of ER morphology in Arabidopsis thaliana2009

    • Author(s)
      R.T.Nakano, R.Matsushima, H.Ueda, Kentaro Tamura, T.Shimada, L.Li, Y.Hayashi, M.Kondo, M.Nishimura, I.Hara-Nishimura
    • Journal Title

      Plant Cell 21

      Pages: 3672-3685

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel membrane fusion-mediated plant immunity against bacterial pathogens2009

    • Author(s)
      N.Hatsugai, S.Iwasaki, Kentaro Tamura, M.Kondo, K.Fuji, K.Ogasawara, M.Nishimura, I.Hara-Nishimura
    • Journal Title

      Genes Dev. 23

      Pages: 2496-2506

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of myosin xi is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells2009

    • Author(s)
      E.Yokota, S.Ueda, Kentaro Tamura, H.Orii, S.Uchi, S.Sonobe, I.Hara-Nishimura, T.Shimmen
    • Journal Title

      J.Exp. Bot. 49

      Pages: 197-212

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A missense mutation in the Arabidopsis COPII coat protein Sec24A induces the formation of clusters of the endoplasmic reticulum and Golgi apparatus.2009

    • Author(s)
      Carmen Faso, et al.
    • Journal Title

      The Plant Cell 21

      Pages: 3655-3671

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ERMO1/GNL1 and ERMO2/SEC24a are required for maintenance of ER morphology in Arabidopsis thaliana.2009

    • Author(s)
      Ryohei Thomas Nakano, et al.
    • Journal Title

      The Plant Cell 21

      Pages: 3672-3685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel membrane fusion-mediated plant immunity against bacterial pathogens.2009

    • Author(s)
      Noriyuki Hatsugai, et al.
    • Journal Title

      Genes & Development 23

      Pages: 2496-2506

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of myosin xi is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells.2009

    • Author(s)
      Etsuo Yokota, et al.
    • Journal Title

      Journal of Experimental Botany 49

      Pages: 197-212

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An Isoform of Myosin XI Is Responsible for the Translocation of Endoplasmic Reticulum in Tobacco Cultured BY-2 Cells2009

    • Author(s)
      E. Yokota, S. Ueda, K. Tamura, H. Orii, S. Uchi, S. Sonobe, I. Hara-Nishimura, T. Shimmen
    • Journal Title

      Journal of Experimental Botany 49

      Pages: 179-212

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of sphingoid long-chain base 1-phosphates (LCB-1PS): functional characterization and expression of ATDPL1 encoding LCB-1P lyase involved in the dehydration stress response in Arabidopsis for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells.2008

    • Author(s)
      M.Nishikawa, K.Hosokawa, M.Ishiguro, H.Minamioka, Kentaro Tamura, I.Hara-Nishimura, Y.Takahashi, K.Shimazaki, H.Imai.
    • Journal Title

      Plant and Cell Physiol. 49

      Pages: 1758-1763

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence2008

    • Author(s)
      M.Yamazaki, T.Shimada, H.Takahashi, Kentaro Tamura, M.Kondo, M.Nishimura, I.Hara-Nishimura
    • Journal Title

      Plant Cell Physiol. 49(2)

      Pages: 142-156

    • NAID

      10027328561

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Degradation of Sphingoid Long-Chain Base 1-Phosphates (LCB-1Ps) : Functional Characterization and Expression of AtDPLl Encoding LCB-1P Lyase Involved in the Dehydration Stress Response in Arabidopsis for the Translocation of Endoplasmic Reticulum in Tobacco Cultured BY-2 Cells2008

    • Author(s)
      M. Xishikawa, K. Hosokawa, M. Ishiguro, H. Minamioka, K. Tamura, I. Hara-Xishimura, Y. Takahashi, K, Shimazaki, H. Imai
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 1758-1763

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis VPS35, a Retromer Component, is Required for Vacuolar Protein Sorting and Involved in Plant Growth and Leaf Senescence2008

    • Author(s)
      M. Yamazaki, T. Shimada, H. Takahashi, K. Tamura, M, Kondo, M. Nishimura, I. Hara-Nishimura
    • Journal Title

      Plant and Cell Physiology 49

      Pages: 142-156

    • NAID

      10027328561

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 西村いくこ シロイヌナズナKAKU1は細胞核の形態に寄与するミオシンである2011

    • Author(s)
      田村謙太郎, 岩渕功誠, 深尾陽一朗, 岡本圭史
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] プロテオミクスで植物の細胞核を知る2010

    • Author(s)
      田村謙太郎
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-19
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 高等植物における核膜孔複合体の動態解析2009

    • Author(s)
      田村謙太郎, 深尾陽一朗, 岩本政明, 原口徳子, 西村いくこ
    • Organizer
      日本植物生理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 高等植物における細胞核の形作りの分子機構2009

    • Author(s)
      田村謙太郎, 西村いくこ
    • Organizer
      第11回オルガネラワークショップ
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic nuclear envelope in Arabidopsis2008

    • Author(s)
      Kentaro Tamura, I. Hara-Nishimura
    • Organizer
      European Endomembrane Meeting
    • Place of Presentation
      Lecce, Italy
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.bot.kyoto-u.ac.jp/j/4_saibou.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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