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Intracellular positioning mechanism of chloroplasts mediated by plant villin

Research Project

Project/Area Number 20570037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOsaka University

Principal Investigator

TAKAGI Shingo  大阪大学, 大学院・理学研究科, 准教授 (10192626)

Research Collaborator TAKAMATSU Hideyasu  大阪大学, 大学院・理学研究科, 大学院生
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords環境応答 / 葉緑体 / アクチン細胞骨格 / カルシウムイオン / 細胞膜ゴースト / シロイヌナズナ / ビリン / ホウレンソウ / 葉緑体アンカー / カルモデュリン / アクチン結合蛋白質
Research Abstract

Advantageous distribution pattern of photosynthesizing organelle chloroplasts is crucial to maintain optimal photosynthesis under different environmental conditions. Redistribution of chloroplasts induced by environmental fluctuations entails de-anchoring, relocation movement, and re-anchoring of chloroplasts. In this study, we revealed that de-anchoring of chloroplasts, the initial step of their redistribution, is brought about through reorganization of actin cytoskeleton under the control of calcium-calmodulin. We further raised a possibility that an actin-binding protein villin is involved in the mechanism.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (49 results)

All 2011 2010 2009 2008 Other

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (32 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Actin-dependent chloroplast anchoring is regulated by Ca^<2+>-calmodulin in spinach mesophyll cells2011

    • Author(s)
      Takamatsu H., Takagi S.
    • Journal Title

      Plant and Cell Physiology

      Volume: 52 Pages: 1973-1982

    • NAID

      10030305534

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic behavior of double-membrane-bound organelles in plant cells2011

    • Author(s)
      Takagi S., Islam M. S., Iwabuchi K.
    • Journal Title

      International Review of Cell and Molecular Biology

      Volume: 286 Pages: 181-222

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Journal Article] 植物オルガネラは忙しい2011

    • Author(s)
      高木慎吾
    • Journal Title

      生産と技術

      Volume: 63 Pages: 74-76

    • NAID

      40018919518

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Journal Article] Actin-based mechanisms for light-dependent intracellular positioning of nuclei and chloroplasts in Arabidopsis2010

    • Author(s)
      Iwabuchi K., Takagi S.
    • Journal Title

      Plant Signaling and Behavior

      Volume: 5 Pages: 1010-1013

    • Related Report
      2010 Final Research Report
  • [Journal Article] Actin reorganization underlies_phototropin-dependent_positioning of nuclei in Arabidopsis leaf cells.2010

    • Author(s)
      Iwabuchi K., Minamino R., Takagi S.
    • Journal Title

      Plant Physiology

      Volume: 152 Pages: 1309-1319

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Co-localization of mitochondria with chloroplasts is a light-dependent reversible response.2010

    • Author(s)
      Islam M.S., Takagi S.
    • Journal Title

      Plant Signaling and Behavior

      Volume: 5 Pages: 146-147

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Actin-based mechanisms for light-dependent intracellular positioning of nuclei and chloroplasts in Arabidopsis.2010

    • Author(s)
      Iwabuchi K., Takagi S.
    • Journal Title

      Plant Signaling and Behavior

      Volume: 5 Pages: 1-4

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Chloroplast anchoring : its implications for the regulation of intracellular chloroplast distribution2009

    • Author(s)
      Takagi S., Takamatsu H., Sakurai-Ozato N.
    • Journal Title

      Journal of Experimental Botany

      Volume: 60 Pages: 3301-3310

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast anchoring : its implications for the regulation of intracellular chloroplast distribution.2009

    • Author(s)
      Takagi S., Takamatsu H., Sakurai-Ozato N.
    • Journal Title

      Journal of Experimental Botany

      Volume: 60 Pages: 3301-3310

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light-dependent intracellular positioning of mitochondria in Arabidopsis thaliana mesophyll cells2009

    • Author(s)
      Islam M.S., Niwa Y., Takagi S.
    • Journal Title

      Plant and Cell Physiology 50

      Pages: 1032-1040

    • NAID

      10027340535

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leaf angle in Chenopodium album is determined by two processes : induction and cessation of petiole curvature2008

    • Author(s)
      Fujita K., Takagi S., Terashima I.
    • Journal Title

      Plant, Cell and Environment 31

      Pages: 1138-1146

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] ホウレンソウ葉肉細胞のアクチン細胞骨格と葉緑体アンカーはCa^<2+>-カルモデュリンによって制御される2011

    • Author(s)
      高松秀安、高木慎吾
    • Organizer
      2011年植物科学談話会
    • Place of Presentation
      大阪市立大学付属植物園
    • Year and Date
      2011-07-17
    • Related Report
      2010 Final Research Report
  • [Presentation] ホウレンソウ葉肉細胞のアクチン細胞骨格と葉緑体アンカーはCa^<2+>-カルモデュリンによって制御される2011

    • Author(s)
      高松秀安、高木慎吾
    • Organizer
      2011年植物科学談話会
    • Place of Presentation
      大阪市立大学付属植物園(交野市)
    • Year and Date
      2011-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of chloroplast positioning by light and CO_22011

    • Author(s)
      Ishida Y., Takagi S.
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      奈良県新公会堂
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ細胞膜ゴーストを用いた葉緑体アンカーの解析2011

    • Author(s)
      貴傳名亮太、高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      2011年生体運動研究合同班会議
    • Place of Presentation
      大阪市立大学
    • Related Report
      2010 Final Research Report
  • [Presentation] ホウレンソウ葉肉細胞のアクチン細胞骨格と葉緑体アンカーはCa^<2+>-カルモデュリンによって制御される2011

    • Author(s)
      高松秀安、高木慎吾
    • Organizer
      2011年生体運動研究合同班会議
    • Place of Presentation
      大阪市立大学(大阪市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ細胞膜ゴーストを用いた葉緑体アンカーの解析2011

    • Author(s)
      貴傳名亮太、高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      2011年生体運動研究合同班会議
    • Place of Presentation
      大阪市立大学(大阪市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Photoregulation of mitochondria/chloroplasts co-localization in Arabidopsis thaliana mesophyll cells2011

    • Author(s)
      Islam M.S., Takagi S.
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      奈良県新公会堂(奈良市)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Regulation of chloroplast positioning by light and CO_22011

    • Author(s)
      Ishida Y., Takagi S.
    • Organizer
      The 5th Asia Oceania Conference on Photobiology
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] イメージングによる細胞内運動の解析2011

    • Author(s)
      高木慎吾
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナの葉表皮細胞における光依存的な伸長制御の解析2010

    • Author(s)
      小川雄右、高木慎吾
    • Organizer
      2010年度日本植物学会近畿支部会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-11-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホウレンソウ葉肉細胞における細胞膜ゴーストを用いた葉緑体アンカーの解析2010

    • Author(s)
      高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      2010年生体運動研究合同班会議
    • Place of Presentation
      中央大学
    • Related Report
      2010 Final Research Report
  • [Presentation] ホウレンソウ葉肉細胞における細胞膜ゴーストを用いた葉緑体アンカーの解析2010

    • Author(s)
      高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      2010年生体運動研究合同班会議
    • Place of Presentation
      中央大学(東京都)
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナ葉における細胞核DNA領域を保持する因子の探索2010

    • Author(s)
      坂本勇貴、高木慎吾
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Photoreceptor systems involved in light-induced redistribution of mitochondria in Arabidopsis thaliana mesophyll cells2010

    • Author(s)
      Islam M.S., Sakamoto W., Takagi S.
    • Organizer
      第16回日本光生物学協会年会
    • Place of Presentation
      大阪大学(吹田市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Light-dependent intracellular positioning of nuclei requires actin reorganization in leaf cells of Arabidopsis thaliana2010

    • Author(s)
      Iwabuchi K., Minamino R., Tamura K., Hara-Nishimura I., Takagi S.
    • Organizer
      2010 Cold Spring Harbor Asia meeting on Plant Biology to Crop Biotechnology
    • Place of Presentation
      Suzhou, China
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナにおける葉緑体の分布に対する背腹性と二酸化炭素濃度の影響2009

    • Author(s)
      石田泰浩、高木慎吾
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学
    • Related Report
      2010 Final Research Report
  • [Presentation] ホウレンソウ葉肉細胞における葉緑体のアンカー機構に対するビリンの関与2009

    • Author(s)
      高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナにおける葉緑体の分布に対する背腹性と二酸化炭素濃度の影響2009

    • Author(s)
      石田泰浩、高木慎吾
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学(山形市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] オオセキショウモにおける青色光受容体フォトトロピンの同定と解析2009

    • Author(s)
      酒井友希、井上晋一郎、原田明子、島崎研一郎、高木慎吾
    • Organizer
      日本植物学会第73回大会
    • Place of Presentation
      山形大学(山形市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Photosynthesis and phototropins play some role in intracellular positioning of mitochondria in Arabidopsis thaliana2009

    • Author(s)
      Islam M.S., Takagi S.
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      紫蘭会館(京都市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification and partial characterization of blue-light photoreceptor phototropins in Vallisneria gigantea2009

    • Author(s)
      Sakai Y, Inoue S., Harada A, Shimazaki K., Takagi S.
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      紫蘭会館(京都市)
    • Related Report
      2009 Annual Research Report
  • [Presentation] ホウレンソウ葉肉細胞における葉緑体のアンカー機構に対するビリンの関与2009

    • Author(s)
      高松秀安、横田悦雄、新免輝男、高木慎吾
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋大学(名古屋市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Possible involvement of phototropins and photosynthesis in light-induced redistribution of mitochondria in Arabidopsis thaliana mesophyll cells2009

    • Author(s)
      Islam M.S., Niwa Y., Takagi S.
    • Organizer
      The 15th International Congress on Photobiology
    • Place of Presentation
      Dusseldorf, Germany
    • Related Report
      2008 Annual Research Report
  • [Presentation] Photoreceptor systems for light-dependent intracellular positioning of mitochondria in Arabidopsis thaliana2009

    • Author(s)
      Islam M.S., Niwa Y., Takagi S.
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Honolulu, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナにおける核光定位運動-基本的特徴と運動の仕組み2009

    • Author(s)
      岩渕功誠、南野亮子、高木慎吾
    • Organizer
      第15回日本光生物学協会年会
    • Place of Presentation
      基礎生物学研究所(岡崎市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナ葉肉細胞プロトプラストの葉緑体の配置に及ぼす二酸化炭素の影響2008

    • Author(s)
      石田泰浩、高木慎吾
    • Organizer
      2008年度日本植物学会近畿支部会
    • Place of Presentation
      神戸大学
    • Year and Date
      2008-12-13
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ葉肉細胞プロトプラストの葉緑体の配置に及ぼす二酸化炭素の影響2008

    • Author(s)
      石田泰浩、高木慎吾
    • Organizer
      2008年度日本植物学会近畿支部会
    • Place of Presentation
      神戸大学(神戸市)
    • Year and Date
      2008-12-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] Light-dependent mitochondrial intracellular positioning in Arabidopsis thaliana mesophyll cells2008

    • Author(s)
      Islam M.S., Hotta T., Niwa Y., Takagi S.
    • Organizer
      Arabidopsis Workshop 2008
    • Place of Presentation
      基礎生物学研究所(岡崎市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナ成熟葉における青色光に依存した核定位運動とアクチン細胞骨格構築変化2008

    • Author(s)
      岩渕功誠、高木慎吾
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知大学(高知市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] シロイヌナズナ成熟葉表皮細胞における核定位位置の解析2008

    • Author(s)
      南野亮子、岩渕功誠、高木慎吾
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知大学(高知市)
    • Related Report
      2008 Annual Research Report
  • [Presentation] Possible involvement of phytochromes in regulation of cytoplasmic motility in Arabidopsis thaliana leaf epidermal cells2008

    • Author(s)
      高木慎吾
    • Organizer
      The 4th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Varanasi, India
    • Related Report
      2008 Annual Research Report
  • [Presentation] Light-induced mitochondrial redistribution in Arabidopsis thaliana palisade cells2008

    • Author(s)
      Islam M.S., Niwa Y., Takagi S.
    • Organizer
      The 4th Asia Oceania Conference on Photobiology
    • Place of Presentation
      Varanasi, India
    • Related Report
      2008 Annual Research Report
  • [Book] Possible involvement of phytochromes in regulation of cytoplasmic motility in Arabidopsis thaliana leaf epidermal cells2011

    • Author(s)
      Sakurai-Ozato, N., Takagi S.
    • Publisher
      Imagination : Editors & Printers (Treatise on Photophysiology)
    • Related Report
      2010 Annual Research Report
  • [Book] Light-induced mitochondrial redistribution in Arabidopsis thaliana palisade cells2011

    • Author(s)
      Islam M.S., Niwa Y., Takagi S.
    • Publisher
      Imagination : Editors & Printers (Treatise on Photophysiology)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/bio_web/lab_page/takagi/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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