Elucidation of the mechanism controlling the formation of photosystem II complex by means of structural analysis of various mutants
| Project/Area Number |
20570038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Okayama University |
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Principal Investigator |
SIN Kenjin Okayama University, 大学院・自然科学研究科, 教授 (60261161)
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| Research Collaborator |
KAMIYA Nobuo 大阪市立大学, 大学院・理学研究科, 教授 (60152865)
UMENA Yasufumi 大阪市立大学, 大学院・理学研究科, 博士研究員
KAWAKAMI Keisuke 岡山大学, 大学院・自然科学研究科, 大学院生
TAKASAKA Kenji 岡山大学, 大学院・自然科学研究科, 大学院生
IWAI Masako 東京理科大学, 理工学部, 助教 (90385526)
IKEUCHI Masahiko 東京大学, 大学院・総合文化研究科, 教授 (20159601)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光合成 / 光化学系II / 酸素発生 / 電子伝達 / 膜タンパク質 / 結晶化 / 構造解析 / 変異体 / 結晶構造解析 / タンパク質複合体 / 膜タンパク / 水分解 |
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| Research Abstract |
In order to elucidate the mechanism controlling the formation of the photosystem II complex, studies on the structures and functions of various low molecular weight subunit-deletion mutants of PSII were carried out. The crystal structure of Ycf12 (Psb30)-deletion mutant confirmed the location of Ycf12, and PSII isolated from a PsbZ-deletion mutant lacked both Ycf12 and PsbK, suggesting the requirement of PsbZ for the stable binding of these two subunits to PSII. In addition, PsbM was shown to be required for maintaining the stability of PSII dimer, while PsbI was required for the formation of PSII dimer but not required for the stability of the dimer.
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Binding and functional properties of five extrinsic proteins in oxygen-evolving Photosystem II from a marine centric diatom, Chaetoceros gracilis.2010
Author(s)
Nagao R., Moriguchi A., Tomo T., Niikura A., Nakajima S., Suzuki T., Okumura A., Iwai M., Shen J.-R., Ikeuchi M., Enam I.
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Journal Title
J.Biol.Chem. 285
Pages: 29191-29199
Related Report
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[Journal Article] Topological analysis of the extrinsic PsbO, PsbP and PsbQ proteins in a green algal PSII complex by cross-linking with a water-soluble carbodiimide.2010
Author(s)
Nagao R., Suzuki T., Okumura A., Niikura A., Iwai M., Dohmae N., Tomo T., Shen J.-R., Ikeuchi M., Enami I.
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Journal Title
Plant Cell Physiol. 51
Pages: 718-727
NAID
Related Report
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[Journal Article] Topological analysis of the extrinsic PsbO, PsbP and PsbQ proteins in a green algal PSII complex by cross-linking with a water-soluble carbodiimide2010
Author(s)
Nagao R, Suzuki T, Okumura A, Niikura A, Iwai M, Dohmae N, Tomo T, Shen J.-R, Ikeuchi M, Enami I.
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Journal Title
Plant Cell Physiol.
Volume: 51
Pages: 718-727
NAID
Related Report
Peer Reviewed
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[Journal Article] Binding and functional properties of five extrinsic proteins in oxygen-evolving Photosystem II from a marine centric diatom. Chaetoceros gracilis2010
Author(s)
Nagao R., Moriguchi A., Tomo T., Niikura A., Nakajima A., Suzuki T., Okumura A., Iwai M., Shen J.-R., Ikeuchi M., Enami I.
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Journal Title
J.Biol.Chem.
Volume: 285
Pages: 29191-29199
Related Report
Peer Reviewed
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