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Study on the molecular mechanism of D1 protein aggregation in photosystem II under light and heat stresses

Research Project

Project/Area Number 20570039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionOkayama University

Principal Investigator

YAMAMOTO Yasushi  Okayama University, 大学院・自然科学研究科, 教授 (40091251)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords光化学系II / D1タンパク質 / 光ストレス / 熱ストレス / FtsHプロテアーゼ / チラコイド / 活性酸素 / 光化学系IIの品質管理 / Ftshプロテアーゼ / 光化学系IIの晶質管理 / 品質管理 / チラコイド膜 / 光合成 / ホウレンソウ / 脂質過酸化 / unstacking / D1タンパク質分解
Research Abstract

The results of our 3 years' research activity are summarized as follows : (1) Under light and heat stresses, lipid peroxidation and the subsequent production of reactive oxygen species take place, which become one of the causes for oxidative damage, aggregation and degradation of the D1 protein. (2) To prevent further damage to the D1 protein under light stess, the thylakoid membranes show structural changes including membrane unstacking. (3) When the photosystem II complexes in the grana are photodamaged under high light, subunits of metalloprotease FtsH forms hexamers at the grana, probably at the grana margin, and carry out degradation of the damaged D1 protein.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (62 results)

All 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (49 results) Remarks (1 results)

  • [Journal Article] Assay of photoinhibition and heat inhibition of photosystem II in higher plants.2010

    • Author(s)
      N.Nijo, Y.Yamamoto, 以下3名
    • Journal Title

      Methods in Mol.Biol. 684 Humana press

      Pages: 201-215

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : FtsH hexamers are localized near Photosystem II at grana for the swift repair of damage.2010

    • Author(s)
      M.Yoshioka, Y.Yamamoto, 以下5名
    • Journal Title

      J.Biol.Chem. 285

      Pages: 41972-41981

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Study on the effects of chloride depletion on photosystem II using different chloride depletion methods.2010

    • Author(s)
      A.Jajoo, Y.Yamamoto, 以下4名
    • Journal Title

      J.Bioenerg.Biomembr. 42

      Pages: 47-53

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assay of Photoinhibiiton and Heat Inhibition of Photosystem II2010

    • Author(s)
      N.Nijo
    • Journal Title

      Methods in Molecular Biology

      Volume: 684 Pages: 201-215

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : FtsH hexamers are localized near Photosystem II at grana for the swift repair of damage2010

    • Author(s)
      M.Yoshioka
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 41972-41981

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study on the effects of chloride depletion on photosystem II using different chloride depletion methods2010

    • Author(s)
      A.Jajoo
    • Journal Title

      J.Bioenerg.Biomembr.

      Volume: 42 Pages: 47-53

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : thylakoid unstacking is necessary to avoid further damage to the D1 protein and to facilitate D1 degradation under light stress in spinach thylakoids.2009

    • Author(s)
      M.Khatoon, Y.Yamamoto, 以下9名
    • Journal Title

      J.Biol.Chem. 284

      Pages: 25343-25352

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality Control of Photosystem II : thylakoid unstacking is necessary to avoid further damage to the D1 protein and facilitate D1 degradation under light stress in spinach thylakoids2009

    • Author(s)
      M.Khatoon
    • Journal Title

      J. Biol. Chem. 284

      Pages: 25343-25352

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : impact of light and heat stresses.2008

    • Author(s)
      Y.Yamamoto, 以下11名
    • Journal Title

      Photosynth.Res. 98

      Pages: 589-608

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : reactive oxygen species are responsible for the damage to Photosystem II under moderate heat stress.2008

    • Author(s)
      A.Yamashita, Y.Yamamoto, 以下6名
    • Journal Title

      J.Biol.Chem. 283

      Pages: 28380-28391

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : impact of light and heat stress2008

    • Author(s)
      Yasusi Yamamoto
    • Journal Title

      Photosynthesis Research 98

      Pages: 589-608

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of Photosystem II : reactive oxygen species are responsible for the damage to Photosystem II under moderate heat stress2008

    • Author(s)
      Amu Yamashita
    • Journal Title

      Journal of Biological Chemistry 283

      Pages: 28380-28391

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II2010

    • Author(s)
      Bjorn Lundin
    • Organizer
      15th International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quality control of Photosystem II : FtsH hexamers are closely associated with Photosystem II at2010

    • Author(s)
      吉岡美保
    • Organizer
      15th International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quality control of Photosystem II : peroxidation of phosphatidylglycerol plays a critical role in2010

    • Author(s)
      Tiffanie Chan
    • Organizer
      15th International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control : Arabidopsis thalianaのキナーゼSTN7/STN8ノックアウトmutantの光ストレス下でのD1タンパク質の分解2010

    • Author(s)
      森田聖也
    • Organizer
      15th International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control:光ストレス下におけるホウレンソウチラコイドのタンパク質酸化2010

    • Author(s)
      清水由梨香
    • Organizer
      15th International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Year and Date
      2010-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quality control of Photosystem II : FtsH hexamers are closely associated with Photosystem II at2010

    • Author(s)
      吉岡美保
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montriol
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II2010

    • Author(s)
      Bjorn Lundin
    • Organizer
      International Conference on Arabidopsis Research
    • Place of Presentation
      横浜
    • Year and Date
      2010-06-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control:損傷D1タンパク質の分解場所とFtsHプロテアーゼの役割2010

    • Author(s)
      吉岡美保
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II complex, access to FtsH protease and stability of FtsH2010

    • Author(s)
      B.Lundin
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : peroxidation of phosphatidylglycerol plays a critical role in the oxidative damage to PSII under moderate heat stress2010

    • Author(s)
      T.Chan
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Pholosystem II : peroxidation of phosphatidylglycerol plays a critical role in2010

    • Author(s)
      Tiffanie Chan
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II2010

    • Author(s)
      Bjorn Lundin
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control : Arabidopsis thalianaのキナーゼSTN7/STN8ノックアウトmutantの光ストレス下でのD1タンパク質の分解2010

    • Author(s)
      森田聖也
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control:光ストレス下におけるホウレンソウチラコイドのタンパク質酸化2010

    • Author(s)
      清水由梨香
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系IIのquality control:光ストレス下でのホウレンソウ葉緑体チラコイド膜上のFtsHプロテアーゼの存在状態解析2010

    • Author(s)
      大橋研介
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 光化学系 IIのquality control:光ストレス下でのホウレンソウ葉緑体チラコイド膜上のFtsHプロテアーゼの存在状態解析2010

    • Author(s)
      大橋研介
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系 IIのquality control:光ストレス下におけるホウレンソウチラコイドのタンパク質酸化2010

    • Author(s)
      清水由梨香
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系 IIのquality control:Arabidopsis thalianaのキナーゼSTN7/STN8ノックアウトmutantの光ストレス下でのD1タンパク質分解2010

    • Author(s)
      森田聖也
    • Organizer
      生物系三学会中国四国支部
    • Place of Presentation
      山口
    • Year and Date
      2010-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Peroxidation of phosphatidylglycerol plays a critical role in the oxidative damage to Photosystem II under moderate heat stress2010

    • Author(s)
      T.Chan
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系 IIのquality control:ホウレンソウチラコイドのunstackingは、光ストレスでのD1タンパク質のダメージを避けD1タンパク質の分解を容易にするために必要である2010

    • Author(s)
      猪名川佳代
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Rate-limiting step for degradation of D1 is the separation of CP43 from Photosystem II2010

    • Author(s)
      B.Lundin
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系 IIのquality control:光・熱ストレスやチラコイド膜での存在場所に依存するFtsHプロテアーゼのサブユニット構造2010

    • Author(s)
      吉岡美保
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control : 光・熱ストレスやチラコイド膜での存在場所に依存するFtsHプロテアーゼのサブユニット構造2010

    • Author(s)
      吉岡美保
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光合成光化学系IIのquality control:グラナにおける六量体FtsHプロテアーゼの役割2010

    • Author(s)
      吉岡美保
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      愛知
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Quality control of Photosystem II:光ストレス下におけるホウレンソウチラコイドのタンパク質酸化2010

    • Author(s)
      清水由梨香
    • Organizer
      15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : Arabidopsis thalianaのキナーゼSTN7/STN8ノックアウトmutantの光ストレス下でのD1タンパク質分解2010

    • Author(s)
      森田聖也
    • Organizer
      15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Peroxidation of phosphatidylglycerol plays a critical role in the oxidative damage to Photosystem II under moderate heat stress2010

    • Author(s)
      T.Chan
    • Organizer
      15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : FtsH hexamers are closely associated with Photosystem II at grana for swift repair of damage2010

    • Author(s)
      吉岡美保
    • Organizer
      15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II complex, access to FtsH protease and stability of FtsH2010

    • Author(s)
      B.Lundin
    • Organizer
      15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : FtsH hexamers are closely associated with Photosystem II at grana for swift repair of damage2010

    • Author(s)
      吉岡美保
    • Organizer
      Plant Biology 2010
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II core D1 protein is regulated by stability of the Photosystem II complex, access to FtsH protease and stability of FtsH2010

    • Author(s)
      B.Lundin
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      横浜
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIの品質管理2009

    • Author(s)
      二條庸好, 山本泰
    • Organizer
      第20回関西光合成研究会
    • Place of Presentation
      奈良
    • Year and Date
      2009-11-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : thylakoid unstacking is necessary to avoid further damage to the D1 protein and also to facilitate D1 degradation under light stress in spinach thylakoids2009

    • Author(s)
      M.Khatoon
    • Organizer
      第50回日本植物生理学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系 IIのquality control:光・熱損傷D1タンパク質の分解に関するFtsHプロテアーゼの分布と存在状態2009

    • Author(s)
      吉岡美保
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Nucleotide dependent processes in the thylakoid lumen of higher plant chloroplast2009

    • Author(s)
      B.Lundin
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : localization and structure properties of the FtsH proteases involved in the degradation of photo- or heat-damaged D1 protein2009

    • Author(s)
      吉岡 美保
    • Organizer
      学内COE「複眼的観点を養うバイオサイエンス教育」年次発表会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Quality control of Photosystem II : thylakoid unstacking is necessary for efficient degradation of the Dl protein under light and heat stresses in spinach thylakoids2009

    • Author(s)
      Mahbuba Khatoon
    • Organizer
      学内COE「複眼的観点を養うバイオサイエンス教育」年次成果発表会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] Nucleotide dependent processes in the thylakoid lumen of higher plant chloroplast2009

    • Author(s)
      Bjorn Lundin
    • Organizer
      第50回日本植物生理学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Quality control of Photosystem II : localization and structure properties of the FtsH proteases involved in the degradation of photo- or heat-damaged D1 protein2009

    • Author(s)
      吉岡 美保
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] Quality control of Photosystem II : thylakoid unstacking is necessary for efficient degradation of the Dl protein under light and heat stresses in spinach thylakoids2009

    • Author(s)
      Mahbuba Khatoon
    • Organizer
      第50回日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光化学系IIのquality control:光および熱で損傷をうけたD1タンパク質の分解に関与するFtsHプロテアーゼの存在状態2008

    • Author(s)
      吉岡美保
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知
    • Year and Date
      2008-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control:ホウレンソウチラコイドからのFtsHプロテアーゼの可溶化と精製の試み2008

    • Author(s)
      猪名川佳代
    • Organizer
      生物系三学会中国四国支部大会
    • Place of Presentation
      東広島
    • Year and Date
      2008-05-17
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control : Synechocystis sp. PCC 6803由来FtsHプロテアーゼの精製2008

    • Author(s)
      網中良太
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Quality control of Photosystem II : relationship between the thylakoid unstacking and degradation of the D1 protein under light and heat stresses in spinach thylakoids2008

    • Author(s)
      M.Khatoon
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control:熱ストレス下でのホウレンソウチラコイド膜のD1タンパク質分解に関与するFtsHの精製2008

    • Author(s)
      吉岡美保
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control:D1ターンオーバーでFtsHプロテアーゼは2つの役割を果たす2008

    • Author(s)
      山下亜夢
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control:熱ストレス下での活性酸素分子の発生、D1タンパク質の分解および膜表在性タンパク質PsbOの遊離2008

    • Author(s)
      二條庸好
    • Organizer
      第49回日本植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2008-03-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIのquality control : ホウレンソウチラコイドからのFtsHプロテアーゼの可溶化と精製の試み2008

    • Author(s)
      猪名川 佳代
    • Organizer
      生物系三学会中国四国支部会広島大会
    • Place of Presentation
      東広島
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光化学系IIのquality control : 光および熱で損傷をうけたDlタンパク質の分解に関与するFtsHプロテアーゼの存在状態2008

    • Author(s)
      吉岡 美保
    • Organizer
      日本植物学会第72回大会
    • Place of Presentation
      高知
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.biol.okayama-u.ac.jp/yamamoto/home.htm

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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