Functional analysis of sporopollenin biosynthetic pathway in pollen exine

• Project/Area Number: 20570047
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ITO Takuya The Institute of Physical and Chemical Research, 化合物ライブラリー評価研究チーム, 専任研究員 (80291912)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: シロイヌナズナ / 花粉壁 / エキシン / 3-Ketoacyl-CoA合成酵素 / BAHDアシル基転移酵素 / スポロポレニン

Research Abstract

The outer layer of pollen wall, which is called exine, is made of "sporopollenin" polymer. But its chemical structure remains unknown because it is extremely resistant against nonoxidative degradation. The representative analyzed function of Arabidopsis 3-ketoacyl-CoA synthases and a BAHD acyl transferase for their involvement in exine formation. A BAHD mutant showed a subtle phenotypic change in exine appearance. The BAHD gene, therefore, is suggested to be involved in exine formation.

Research Products

• [Journal Article] Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallelwith cortical microtubules. (2010)
  • Author(s): Yoneda, A., Ito, T., Higaki, T., Kutsuna, N., Saito, T., Ishimizu, T., Osada, H., Hasezawa, S., Matsui, M., Demura, T.
  • Journal Title: Plant Journal 64 Pages: 657-667
  • Peer Reviewed
• [Journal Article] Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallelwith cortical microtubules. (2010)
  • Author(s): Yoneda, A., Ito, T., Higaki, T., Kutsuna, N., Saito, T., Ishimizu, T., Osada, H., Hasezawa, S., Matsui, M., Demura, T.
  • Journal Title: Plant Journal Volume: 64 Pages: 657-667
  • Peer Reviewed
• [Presentation] 花粉成熟に必須なシロイヌナズナMS1転写因子が制御するアシル基転移酵素遺伝子の機能解析 (2009)
  • Author(s): 伊藤卓也、松田史生、峠隆之、長田裕之、斉藤和季、篠崎一雄
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Remarks] ホームページ等