Analysis of ABA response network using novel ABA response mutants of Arabidopsis

• Project/Area Number: 20570050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HIRAYAMA Takashi The Institute of Physical and Chemical Research, 資源植物科学研究所, 教授 (10228819)
• Co-Investigator(Renkei-kenkyūsha): UMEZAWA Taishi 理化学研究所, 植物科学研究センター, 研究員 (70342756)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 植物ホルモン / アブシジン酸 / 情報伝達機構 / リン酸化制御 / RNA制御 / タンパク質分解制御 / PP2C / PPR / ミトコンドリア / ストレス応答 / ABA情報伝達経路 / SnRK2 / ABA受容体 / 免疫沈降実験 / 酵母two-hybrid実験 / BiFC / アブシジン酸応答 / 2C型脱リン酸化酵素 / プロテアソーム複合体 / polyA鎖長制御 / 抑制変異株

Research Abstract

Molecular genetic and molecular biological analyses of several novel Arabidopsis mutants that impair the abscisic acid (ABA)-response allowed us to describe the ABA signal transduction pathway from perception to nuclear events and find the novel relation between ABA response and mitochondrial function. These findings offer a new avenue for understanding the cellular response mechanisms to ABA.

Research Products

• [Journal Article] アブシジン酸 (2011)
  • Author(s): 上野琴巳
  • Journal Title: 化学と生物 Volume: 48 Pages: 555-563
• [Journal Article] エチレン、ジベレリン、ジャスモン酸、ストリゴラクトン (2011)
  • Author(s): 王敬銘
  • Journal Title: 化学と生物 Volume: 48 Pages: 637-642
• [Journal Article] Research on plant abiotic stress responses in the post-genome era : past, present, and future. (2010)
  • Author(s): Hirayama, T., Shinozaki, K.
  • Journal Title: Plant J. 61 Pages: 1041-1052
  • Peer Reviewed
• [Journal Article] ABA Hypersensitive Germination2-1 causes the activation of both abscisic acid and salicylic acid responses in Arabidopsis (2010)
  • Author(s): Nishimura, N., Okamoto, M., Narusaka, M., Yasuda, M., Nakashita, H., Shinozaki, S., Narusaka Y., Hirayama, T.
  • Journal Title: Plant Cell Physiol. 50 Pages: 2112-2122
  • NAID: 10027345088
  • Peer Reviewed
• [Journal Article] The PP2C-SnRK2 complex: the central regulator of an abscisic acid signaling pathway (2010)
  • Author(s): Hirayama, T., Umezawa, T.
  • Journal Title: Plant Signaling & Behav. 5 Pages: 160-163
  • Peer Reviewed
• [Journal Article] The PP2C-SnRK2 complex : the central regulator of an abscisic acid signaling pathway (2010)
  • Author(s): Takashi Hirayama
  • Journal Title: Plant Signaling & Behavior Volume: 5 Pages: 160-163
  • Peer Reviewed
• [Journal Article] Research on plant abiotic stress responses in the post-genone era : past.present.and future (2010)
  • Author(s): Takashi Hirayama
  • Journal Title: Plant Journal Volume: 61 Pages: 1041-1052
  • Peer Reviewed
• [Journal Article] A DNA-binding surface of SP011-1. An Arabidopsis SP011 orthologue required for normal meiosis (2010)
  • Author(s): Yasunori Shingu
  • Journal Title: FEBS Journal Volume: 277 Pages: 2360-2374
  • Peer Reviewed
• [Journal Article] The PP2C-SnRK2 complex : the central regulator of an abscisic acidsign aling pathway (2010)
  • Author(s): Takashi Hirayama
  • Journal Title: Plant Signaling & Behavior (In press)
• [Journal Article] Research on plant abiotic stress responses in the post-genome era : past, present, and future (2010)
  • Author(s): Takashi Hirayama
  • Journal Title: Plant Journal (In press)
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Takashi Kuromori
  • Journal Title: The Handbook of Plant Mutagenesis and Mutant Screening(edited by K.Meksem and G.Kahl)(Wiley-Blackwell-VCH) Pages: 17-28
• [Journal Article] Type 2C Protein Phosphatases Directly Regulate Abscisic Acid-Activated Protein Kinases in Arabidopsis (2009)
  • Author(s): Umezawa. T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishitani, Y., Hirayama, T., Shinozaki, K.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 17588-17593
  • Peer Reviewed
• [Journal Article] Metabolic movement upon abscisic acid and salicylic acid combined treatments (2009)
  • Author(s): Okamoto, M., Tsuboi, Y., Chikayama, E., Kikuchi, J., Hirayama, T.
  • Journal Title: Plant Biotch. 26 Pages: 551-560
  • NAID: 10026136750
  • Peer Reviewed
• [Journal Article] Type 2C Protein Phosphatases Directly Regulate Abscisic Acid-Activated Protein Kinases in Arabidopsis (2009)
  • Author(s): Taishi Umezawa
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 17588-17593
  • Peer Reviewed
• [Journal Article] Metabolic movement upon abscisic acid and salicylic acid combined treatments (2009)
  • Author(s): Mami Okamoto
  • Journal Title: Plant Biotechnology 26 Pages: 551-560
  • Peer Reviewed
• [Journal Article] The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization (2009)
  • Author(s): Shimpei Hayashi
  • Journal Title: Plant Cell Physiol. 49 Pages: 1522-1535
  • Peer Reviewed
• [Journal Article] The regulatory network of plant responses to abscisic acid
  • Author(s): Taishi Umezawa
  • Journal Title: Advances in Botanical Research Volume: (in press)
  • Peer Reviewed
• [Presentation] ABA関連PP2C AHG1,AHG3特異的相互作用因子の解析 (2011)
  • Author(s): 牛山翔
  • Organizer: 第52回植物生理学会年会(注;震災により学会は開催されなかったが、学会長より開催したとみなす旨の宣言がなされた)
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] ABA関連PP2C AHG1, AHG3特異的相互作用因子の解析 (2011)
  • Author(s): 牛山翔
  • Organizer: 日本植物生理学会年会(震災で学会は中止となったが、発表がされたと見なされた)
  • Year and Date: 2011-03-21
• [Presentation] 多重ホルモン処理における遺伝子発現解析 (2011)
  • Author(s): 岡本真美
  • Organizer: 日本植物生理学会年会(震災で学会は中止となったが、発表がされたと見なされた)
  • Year and Date: 2011-03-20
• [Presentation] 植物ホルモンの働きとストレス応答 (2011)
  • Author(s): 平山隆志
  • Organizer: 第27回資源植物科学シンポジウム、第3回植物ストレス科学研究シンポジウム
  • Place of Presentation: 倉敷
  • Year and Date: 2011-03-07
• [Presentation] シロイヌナズナpolyA分解酵素AHG2/PARNの機能と細胞応答統御機構 (2010)
  • Author(s): 平山隆志
  • Organizer: シンポジウム RNAから植物を考える
  • Place of Presentation: 札幌
  • Year and Date: 2010-08-26
• [Presentation] The PP2C-SnRK2 complex : A central regulatory module of protein phosphorylation networks in abscisic acid signaling. (2010)
  • Author(s): 梅澤泰史
  • Organizer: 21th International Conference of Arabidopsis Research.
  • Place of Presentation: Yokohama
  • Year and Date: 2010-06-08
• [Presentation] Analysis of a PARN germinati deficient mutant, ABA hypersensitive on2-1, of Arabidopsis. (2010)
  • Author(s): 平山隆志
  • Organizer: 21th International Conference of Arabidopsis Research.
  • Place of Presentation: Yokohama
  • Year and Date: 2010-06-08
• [Presentation] Analysis of a PARN deficient mutant, ABA hypersensitive germination2-1, of Arabidopsis. (2010)
  • Author(s): 平山隆志
  • Organizer: 19th CDB Meeting
  • Place of Presentation: Kobe
  • Year and Date: 2010-05-11
• [Presentation] 蛋白質のリン酸化・脱リン酸化が支配する植物ホルモンアブシジン酸のシグナル伝達経路 (2010)
  • Author(s): 梅澤泰史
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-21
• [Presentation] Protein Phosphatases 2C Directly Regulate Abscisic Acid-Activated Prote in Kinases in Arabidopsis (2010)
  • Author(s): 梅澤泰志
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本県、熊本市
  • Year and Date: 2010-03-21
• [Presentation] Analysis of suppressor mutants of PARN deficient mutant ABA hypersensitive germination2-1. (2010)
  • Author(s): 平山隆志
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナのオミクスデータを利用した植物ホルモンによる代謝制御の一斉解析 (2010)
  • Author(s): 佐々木江理子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本県、熊本市
  • Year and Date: 2010-03-20
• [Presentation] シロイヌナズナの乾燥ストレス応答に関わるプロテインホスファターゼHAIの解析 (2010)
  • Author(s): 仲宗根尚子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本県、熊本市
  • Year and Date: 2010-03-20
• [Presentation] Analysis of a PARN deficient mutant. ABA hypersensitive germination2-1, of Arabidopsis (2010)
  • Author(s): Takashi Hirayama
  • Organizer: 21st International Conference of Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
• [Presentation] Protein Phosphatases 2C Directly Regulate Abscisic Acid-Activated Protein Kinases in Arabidopsis. (2009)
  • Author(s): 梅澤泰史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] Protein Phosphatases 2C Directly Regulate Abscisic Acid-Activated Prote in Kinases in Arabidopsis (2009)
  • Author(s): 平山隆志
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川県、横浜市
  • Year and Date: 2009-12-11
• [Presentation] Physical and functional interactions between 2C-type protein phosphatases and SnRK2 protein kinases in ABA signaling (2009)
  • Author(s): 梅澤泰志
  • Organizer: IPMB Congress
  • Place of Presentation: アメリカ、セントルイス
  • Year and Date: 2009-10-28
• [Presentation] Physical and functional interactions between 2C-type protein phosphatases and SnRK2 protein kinases in ABA signaling (2009)
  • Author(s): 梅澤泰史
  • Organizer: IPMB Congress
  • Place of Presentation: St Louis, USA
  • Year and Date: 2009-10-26
• [Presentation] PARN発現低下変異ABA hypersensitive germiantion2-1の抑制変異株の分離と解析 (2009)
  • Author(s): 平山隆志
  • Organizer: 第11回日本RNA学会年会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-07-28
• [Presentation] Analysis of suppressor mutants of a PARN deficient mutant, ABA hypersensitive germination2-1. (2009)
  • Author(s): 平山隆志
  • Organizer: 20th International Conference on Arabidopsis Research
  • Place of Presentation: Edinburgh, UK.
  • Year and Date: 2009-07-01
• [Presentation] アブシジン酸シグナル伝達におけるSnRK2プロテインキナーゼとPP2Cプロテインホスファターゼの相互作用 (2009)
  • Author(s): 梅澤泰史
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-23
• [Presentation] 植物の環境応答機構と細胞機能 (2009)
  • Author(s): 平山隆志
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 植物の環境応答機構と細胞機能 (2009)
  • Author(s): 平山隆志
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] 新規ABA関連遺伝子座の探索とABA関連因子の機能解析の試み (2009)
  • Author(s): 石出真有美
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] ABA高感受性を示す新しいプロテアソーム変異体ahg12の解析 (2009)
  • Author(s): 林晋平
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] ABA高感受性を示す新しいプロテアソーム変異体ahg12の解析 (2009)
  • Author(s): 林晋平
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] 多次元NMR法を用いた植物ホルモンによる代謝物動態情報の抽出 (2009)
  • Author(s): 岡本真美
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] シロイヌナズナPARN/AHG2の標的mRNAの探索 (2008)
  • Author(s): 平山隆志
  • Organizer: 第10回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-23
• [Presentation] シロイヌナズナPARN/AHG2の標的mRNAの探索 (2008)
  • Author(s): 平山隆志
  • Organizer: 日本RNA学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-07-23
• [Book] Ds transposon mutant lines for saturation mutagenesis of Arabidopsis genome, in The Handbook of Plant Mutagenesis and Mutant Screening (edited by K. Meksem and G. Kahl)(Molecular Plant Biology Handbook Series, Wiley-Blackwell-VCH) (2010)
  • Author(s): Kuromori, T., Hirayama T.
• [Book] "Ds transposon mutant lines for saturation mutagenesis of Arabidopsis genome "in" The Handbook of Plant Mutation Screening" (2009)
  • Author(s): Takashi Kuromori
  • Publisher: Wiley-Blackwell-VCH(印刷中)
• [Remarks] ホームページ
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• [Remarks] プレスリリース
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• [Remarks] RIKEN Research
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  • URL: http://www.tsurumi.yokohama-cu.ac.jp/embiol/j/member/hirayama.html