• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A study of neuronal differentiation of sea cucumber by the comprehensive analysis of neuropeptides.

Research Project

Project/Area Number 20570057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionKyushu University

Principal Investigator

YOSHIKUNI Michiyasu  Kyushu University, 大学院・農学研究院, 教授 (50210662)

Co-Investigator(Renkei-kenkyūsha) OHNO Kaoru  自然科学研究機構, 基礎生物学研究所, 教 (10260035)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords比較内分泌 / 生体分子 / タンパク質 / 生理活性物質 / 棘皮動物 / プロテオーム / 神経 / 機能分化 / 蛋白質 / プロテオミクス / 卵成熟
Research Abstract

EST analysis and mass spectrometry revealed the low quality of neural tissues prepared by a conventional method. A complete dehydration could suppress the degradations of RNAs and peptides during microdissection. The ratio of a neural peptide mRNA and EF1a mRNA was improved over several dozen folds. A new peptide, Cubifirn, was identified as a potent neural factor to induce oocyte maturation and spawning.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (54 results)

All 2011 2010 2009 2008 Other

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (39 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Spawning induced by cubifrin in the Japanese common sea cucumber, Apostichopus japonicus.2010

    • Author(s)
      Fujiwara A, Yamano K, Ohno K, Yoshikuni M.
    • Journal Title

      Fisheries. Science. 76

      Pages: 795-801

    • NAID

      10026642436

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of the major yolk protein of the Japanese common sea cucumber (Apostichopus japonicus) and its expression profile during ovarian development.2010

    • Author(s)
      Fujiwara A, Unuma T, Ohno K, Yamano K.
    • Journal Title

      Comparative Biochemistry and Physiology., Part A. 155

      Pages: 34-40

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spawning induced by cubifrin in the Japanese common sea cucumber, Apostichopus japonicus.2010

    • Author(s)
      藤原篤志、山野恵祐、大野薫、吉国通庸
    • Journal Title

      Fisheries Science

      Volume: 76 Pages: 795-801

    • NAID

      10026642436

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 棘皮動物の生殖を司る神経ペプチド2010

    • Author(s)
      吉国通庸
    • Journal Title

      比較内分泌学会誌

      Volume: 36 Pages: 25-31

    • NAID

      10025990243

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 棘皮動物の生殖を司る神経ペプチド2010

    • Author(s)
      吉国通庸
    • Journal Title

      比較内分泌学会誌 36(136)

      Pages: 25-31

    • NAID

      10025990243

    • Related Report
      2009 Annual Research Report
  • [Journal Article] A relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera.2009

    • Author(s)
      Mita M, Yoshikuni M, Ohno K, Shibata Y, Paul-Prasanth B, Pitchayawasin S, Isobe M, Nagahama Y.
    • Journal Title

      Proceedings of the National Academy of Sciences of the USA 106

      Pages: 9507-9512

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal peptides induce oocyte maturation and gamete spawning of sea cucumber, Apostichopus japonicus.2009

    • Author(s)
      Kato S, Tsurumaru S, Taga M, Yamane T, Shibata Y, Ohno K, Fujiwara A, Yamano K, Yoshikuni M.
    • Journal Title

      Developmental Biology. 326

      Pages: 169-176

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera2009

    • Author(s)
      吉国通庸, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106(23)

      Pages: 9507-9512

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 棘皮動物の生殖を司る神経ペプチド2009

    • Author(s)
      吉国通庸
    • Journal Title

      ペプチド学会 News Letter 73

      Pages: 1-4

    • NAID

      10025990243

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Neuronal peptides induce oocyte maturation and gamete spawning of sea cucumber, Apostichopus japonicus.2009

    • Author(s)
      Kato S., et al.
    • Journal Title

      Developmental Biology 326

      Pages: 169-176

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Comprehensive analysis of neuropeptides in marine invertebrates.2011

    • Author(s)
      吉国通庸
    • Organizer
      第2回JAMBIOフォーラム
    • Place of Presentation
      静岡
    • Year and Date
      2011-01-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Comprehensive Analysis of Neuropeptides in Marine Invertebrates2011

    • Author(s)
      吉国通庸、加藤愼一、大野薫、宇佐美剛志、松本才絵、淡路雅彦、山野恵祐
    • Organizer
      The 2^<nd> Forum of JAMBIO (Japan Association for Marine Biology)
    • Place of Presentation
      下田(招待講演)
    • Year and Date
      2011-01-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 棘皮動物の生殖の制御-最近の新しい展開2011

    • Author(s)
      吉国通庸
    • Organizer
      第3回性差生物医学研究会
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2011-01-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 棘皮動物における卵成熟のホルモン制御2010

    • Author(s)
      吉国通庸
    • Organizer
      第35回日本比較内分泌学会シンポジウム「無脊椎動物における性と生殖の制御」
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-18
    • Related Report
      2010 Final Research Report
  • [Presentation] アカウニ生殖腺刺激ホルモン放出ホルモン様ペプチドの遺伝子発現と生理作用の解析2010

    • Author(s)
      山野恵祐、藤原篤志、中村昭文、大野薫、吉国通庸
    • Organizer
      日本比較内分泌学会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 棘皮動物における卵成熟のホルモン制御2010

    • Author(s)
      吉国通庸、大野薫、山野恵祐
    • Organizer
      日本比較内分泌学会
    • Place of Presentation
      静岡(招待講演)
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海産無脊椎動物神経ペプチドの網羅的解析の試み2010

    • Author(s)
      吉国通庸
    • Organizer
      第81回日本動物学会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 海産無脊椎動物新鋭ペプチドの網羅的解析の試み2010

    • Author(s)
      吉国通庸、加藤愼一、大野薫、宇佐美剛志、山野恵祐、淡路雅彦、松本才絵
    • Organizer
      日本動物学会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] クビフリンを用いたin vitro卵成熟誘起によるマナマコ採卵技術の開発-II2010

    • Author(s)
      山野恵祐
    • Organizer
      平成22年度日本水産学会秋季大会
    • Year and Date
      2010-09-22
    • Related Report
      2010 Final Research Report
  • [Presentation] クビフリンを用いたin vitro卵成熟誘起によるマナマコ採卵技術の開発-II2010

    • Author(s)
      山野恵祐、藤原篤志、中村昭文、大野薫、吉国通庸
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comprehensive Analysis of Neuropeptides in Marine Invertebrates.2010

    • Author(s)
      吉国通庸、加藤愼一、大野薫、宇佐美剛志、松本才絵、淡路雅彦
    • Organizer
      The Pre-Symposium of the 2011 International Congress of Comparative Physiology and Biochemistry
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2010-07-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] The role of neuropeptides in inducing gamete spawning of echinoderm.2010

    • Author(s)
      吉国通庸
    • Organizer
      第11回国際精子学シンポジウム
    • Place of Presentation
      沖縄
    • Year and Date
      2010-06-24
    • Related Report
      2010 Final Research Report
  • [Presentation] The Role of Neuropeptides in Inducing Gamete Spawning of Echinoderm2010

    • Author(s)
      吉国通庸、山野恵祐、藤原篤志、加藤愼一、山根知樹、大野薫
    • Organizer
      The 11^<th> Internal Symposium of Spermatology
    • Place of Presentation
      沖縄
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 無脊椎動物神経ペプチドの網羅的解析の試み2010

    • Author(s)
      吉国通庸、加藤愼一、大野薫、宇佐美剛志
    • Organizer
      日本動物学会九州支部大会
    • Place of Presentation
      福岡
    • Year and Date
      2010-05-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] ナマコの卵成熟と産卵2010

    • Author(s)
      山野恵祐
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ナマコの卵成熟と産卵(招待講演)2010

    • Author(s)
      山野恵祐, 吉国通庸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(駒場)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 棘皮動物の生殖を司る神経ペプチド2009

    • Author(s)
      吉国通庸
    • Organizer
      第34回日本比較内分泌学会シンポジウム「新たな生物材料や分子が導く一般解と特殊解」
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] クビフリンを用いたin vitro卵成熟誘起によるマナマコ採卵技術の開発2009

    • Author(s)
      山野恵祐
    • Organizer
      平成21年度日本水産学会秋季大会
    • Year and Date
      2009-09-29
    • Related Report
      2010 Final Research Report
  • [Presentation] マナマコクビフリンの作用機構の解析:クビフリンアナログの作用2009

    • Author(s)
      吉国通庸
    • Organizer
      第80回日本動物学会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Spawning behavior of Japanese sea cucumber (Apostichopus japonicus) induced by a neuropeptide, Cubifrin.2009

    • Author(s)
      吉国通庸
    • Organizer
      第16回国際比較内分泌学会議
    • Place of Presentation
      HongKong.
    • Year and Date
      2009-06-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Proteome analysis of buccal ring nerve and radial nerve of Japanese common sea cucumber, Apostichopus japonicus.2009

    • Author(s)
      鶴丸早織
    • Organizer
      16回国際比較内分泌学会議
    • Place of Presentation
      HongKong.
    • Year and Date
      2009-06-22
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of neural peptides inducing oocyte maturation of Japanese common sea cucumber, Apostichopus japonicus.2009

    • Author(s)
      加藤愼一
    • Organizer
      16回国際比較内分泌学会議
    • Place of Presentation
      HongKong.
    • Year and Date
      2009-06-22
    • Related Report
      2010 Final Research Report
  • [Presentation] In vitro oocyte maturation induced by a neuropeptide in the Japanese common sea cucumber and its application to aquaculture.2009

    • Author(s)
      山野恵祐
    • Organizer
      16回国際比較内分泌学会議
    • Place of Presentation
      HongKong.
    • Year and Date
      2009-06-22
    • Related Report
      2010 Final Research Report
  • [Presentation] マナマコの放卵・放精を誘起する神経ペプチドの同定2009

    • Author(s)
      吉国通庸
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] 卵成熟誘起能をもつマナマコ神経ペプチドの作用経路2009

    • Author(s)
      山野恵祐
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] マナマコの放卵・放精を誘起する神経ペプチドの同定2009

    • Author(s)
      吉国通庸
    • Organizer
      平成21年度日本水産学会春季大会
    • Year and Date
      2009-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 卵成熟誘起能をもつマナマコ神経ペプチドの作用経路2009

    • Author(s)
      山野恵祐
    • Organizer
      平成21年度日本水産学会春季大会
    • Year and Date
      2009-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] マナマコ周口神経の卵成熟及び放卵・放精誘起活性成分の解明2009

    • Author(s)
      吉国通庸
    • Organizer
      海産無脊椎動物研究会-基礎から応用への展開
    • Place of Presentation
      愛媛大学南予水産研究センター
    • Year and Date
      2009-03-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Spawning behavior of Japanese common sea cucumber(apostichopus japonicus)induced by a neuropeptide, Cubifrin.(招待講演)2009

    • Author(s)
      吉国, 大野, 山野
    • Organizer
      16^<th> International Symposium of Comparative Endocrinology(HongKong)
    • Place of Presentation
      The University of HongKong
    • Related Report
      2009 Annual Research Report
  • [Presentation] 棘皮動物の生殖を司るペプチド(招待講演)2009

    • Author(s)
      吉国, 三田, 大野, 山野
    • Organizer
      第34回日本比較内分泌学会第31回日本比較生理生化学会合同大会
    • Place of Presentation
      千里ライフサイエンスセンター
    • Related Report
      2009 Annual Research Report
  • [Presentation] ナマコクビフリンの作用解析:クビフリンアナログの作用2009

    • Author(s)
      吉国, 加藤, 鶴丸, 山根, 大野, 藤原, 山野
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡グランメッセ
    • Related Report
      2009 Annual Research Report
  • [Presentation] クビフリンを用いた in vitro 卵成熟誘起によるマナマコ採卵技術の開発2009

    • Author(s)
      藤原, 山野, 山根, 吉国
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      盛岡
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of neural peptides inducing oocyte maturation of Japanese common sea cucumber, Apostichopus japonicus2009

    • Author(s)
      Kato, Tsurumaru, Taga, Yamane, Yoshikuni, Shibata, Ohno, Fujiwara, Yamano
    • Organizer
      16^<th> International Symposium of Comparative Endocrinology(HongKong)
    • Place of Presentation
      The University of HongKong
    • Related Report
      2009 Annual Research Report
  • [Presentation] Proteome analysis of buccal ring nereve and radial nerve of Japanese common sea cucumber, Apostichopus japonicus2009

    • Author(s)
      鶴丸, 山根, 加藤, 吉国
    • Organizer
      16^<th> International Symposium of Comparative Endocrinology (HongKong)
    • Place of Presentation
      The University of HongKong
    • Related Report
      2009 Annual Research Report
  • [Presentation] In vitro oocyte maturation induced by a neuropeptide in the Japanese common sea cucumber and its application to aquaculture2009

    • Author(s)
      山野, 藤原, 山根, 吉国
    • Organizer
      16^<th> International Symposium of Comparative Endocrinology (HongKong)
    • Place of Presentation
      The University of HongKong
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of genes expressed at the early stage of ovarian development of the Japanese common sea cucumber2009

    • Author(s)
      Fujiwara, Yamano, nakamura, Usami, Shibata, Ohno, Yoshikuni
    • Organizer
      マリンゲノミクス国際シンポジウム
    • Place of Presentation
      沖縄
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of genes expressed at the early stage of ovarian development of the red sea urchin2009

    • Author(s)
      Yamano, Fujiwara, Nakamura, Unuma, Urawa, Ohno, Yoshikuni
    • Organizer
      マリンゲノミクス国際シンポジウム
    • Place of Presentation
      沖縄
    • Related Report
      2009 Annual Research Report
  • [Presentation] マナマコの卵成熟を誘起する神経ペプチドの同定2008

    • Author(s)
      吉国通庸
    • Organizer
      第33回日本比較内分泌学会
    • Place of Presentation
      広島
    • Year and Date
      2008-12-05
    • Related Report
      2010 Final Research Report
  • [Presentation] マナマコ卵成熟を誘起する生理活性物質の単離と同定2008

    • Author(s)
      吉国通庸
    • Organizer
      第79回日本動物学会
    • Place of Presentation
      福岡
    • Year and Date
      2008-09-05
    • Related Report
      2010 Final Research Report
  • [Book] 第3章 成熟・産卵,「ナマコ-その生物・産業・文化」(高橋明義編)2011

    • Author(s)
      吉国通庸
    • Publisher
      成山堂in press
    • Related Report
      2010 Final Research Report
  • [Book] 第1章 海洋生物のホルモン,「天然物化学-海洋生物編」2008

    • Author(s)
      吉国通庸
    • Publisher
      APC出版
    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.kyushu-u.ac.jp/asweb/jikkensho/home.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.kyushu-u.ac.jp/asweb/jikkensho/home.html

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] マナマコの放卵・放精誘起剤、およびそれを用いたナマコの生産方法2008

    • Inventor(s)
      吉国通庸、大野薫、山野恵祐、淡路雅彦、松本才絵、藤原篤志
    • Filing Date
      2008-08-26
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi