| Project/Area Number |
20570062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Nippon Sport Science University |
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Principal Investigator |
OSAFUNE Tetsuaki Nippon Sport Science University, 体育学部, 教授 (70074630)
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| Co-Investigator(Kenkyū-buntansha) |
EHARA Tomoko 東京医科大学, 医学部, 助手 (40468658)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Euglena / immunoelectron microscopy / LHCP II / Golgi body / plastid / Golgi / 免疫電顕法 / LHCPII / ゴルジ装置 / ユーグレナ / ピレノイド / ストレプトマイシン / ゴルジ体 / 光合成タンパク質 / 葉緑体 / 急速凍結置換固定法 / 植物 |
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| Research Abstract |
Immunoelectron microscopy of wax-rich cells of Euglena gracilis Z using anti-LHCP II antibody and protein A-gold shows LHCP II first in the Cos, Golgi body and Golgi-derived vesicle by the thylakoids of the plastid. Golgi-derived vesicle fuses with the outermost membrane of the Euglena plastid. In the presence of 0.1% Streptomycin have plastids in which the disappearance of the prolamellar body (PLB) is inhibited ; a paracrystalline body is formed in close proximity to the the PLB and pyrenoid which shows an immunoreaction with LHCP II antibody, but the immunoreaction is absent from the thylakoids. Thus conditions in the aerated wax-rich cells allow an unusual accumulation of LHCP II apoprotein at Streptomycin blocks the distribution of this apoprotein to the thylakoids.
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