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Identification of novel ghrelin receptor in fish and clarification of the relationship between the receptor and biological action.

Research Project

Project/Area Number 20570065
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

KAIYA Hiroyuki  National Cardiovascular Center Research Institute, 生化学部, 室長 (40300975)

Co-Investigator(Kenkyū-buntansha) MIYAZATO Mikiya  独立行政法人国立循環器病研究センター, 生化学部, 部長 (50291183)
Co-Investigator(Renkei-kenkyūsha) KANGAWA Kenji  独立行政法人国立循環器病研究センター, 所長 (00112417)
MATSUDA Kouhei  富山大学, 理工学部, 教授 (60222303)
KITAZAWA Takio  酪農学園大学, 獣医学部, 教授 (50146338)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsグレリン / グレリン受容体 / 魚類 / キンギョ / 受容体 / 脳 / 腸 / 内在性 / ペプチド / 精製 / GHS-R1a / GHS-R2a / 変異体 / ゲノム遺伝子 / 組織分布 / cDNA
Research Abstract

In this study, we cloned cDNAs for four functional ghrelin receptors (GHS-R1a-1, 1a-2, 2a-1 and 2a-2) that composed of 360, 360, 366 and 367 amino acids, respectively in goldfish brain. Each receptor mRNA showed tissue-specific expression, suggesting that each receptor mediates tissue-specific action of ghrelin in goldfish. One species of ghrelin receptor, GHS-R1a has been known to be present in vertebrates other than fish examined so far. We tried to isolate new ligands for the new functional GHS-Ra, which is the novel type of ghrelin receptor, from tissue extracts of goldfish brain and intestine, but unfortunately the new ligand was not able to find out during the course of this study.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (31 results)

All 2010 2009 2008

All Journal Article (24 results) (of which Peer Reviewed: 24 results) Presentation (7 results)

  • [Journal Article] Two functional growth hormone secretagogue receptor (ghrelin receptor) type 1a and 2a in goldfish, Carassius auratus.2010

    • Author(s)
      Kaiya H, Miura T, Matsuda K, Miyazato M, Kangawa K.
    • Journal Title

      Mol Cell Endocrinol. 327(1-2)

      Pages: 25-39

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin affects carbohydrate-glycogen metabolism via insulin inhibition and glucagon stimulation in the zebrafish (Danio rerio) brain.2010

    • Author(s)
      Cruz SA, Tseng YC, Kaiya H, Hwang PP.
    • Journal Title

      Comp Biochem Physiol A Mol Integr Physiol. 156(2)

      Pages: 190-200

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin decreases food intake in juvenile rainbow trout (Oncorhynchus mykiss) through the central anorexigenic corticotropin-releasing factor system.2010

    • Author(s)
      Jonsson E, Kaiya H, Bjornsson BT.
    • Journal Title

      Gen Comp Endocrinol. 166(1)

      Pages: 39-46

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two functional growth hormone secretagogue receptor (ghrelin receptor) type 1a and 2a in goldfish, Carassius auratus.2010

    • Author(s)
      Kaiya H, Miura T, Matsuda K, Miyazato M, Kangawa K.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 327 Pages: 25-39

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin affects carbohydrate-glycogen metabolism via insulin inhibition and glucagon stimulation in the zebrafish (Danio rerio) brain.2010

    • Author(s)
      Cruz SA, Tseng YC, Kaiya H, Hwang PP
    • Journal Title

      Comparative Biochemistry and Physiology A

      Volume: 156 Pages: 190-200

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei.2010

    • Author(s)
      Kaiya H, Kodama S, Ishiguro K, Matsuda K, Uchiyama M, Miyazato M, Kangawa K.
    • Journal Title

      BMC Biochemistry

      Volume: 10 Pages: 30-30

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei.2009

    • Author(s)
      Kaiya H, Kodama S, Ishiguro K, Matsuda K, Uchiyama M, Miyazato M, Kangawa K.
    • Journal Title

      BMC Biochem. 10

      Pages: 30-30

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and genomic sequence of a ghrelin receptor (GHS-R)-like receptor in the Mozambique tilapia, Oreochromis mossambicus.2009

    • Author(s)
      Kaiya H, Riley LG, Janzen W, Hirano T, Grau EG, Miyazato M, Kangawa K.
    • Journal Title

      Zoolog Sci. 26(5)

      Pages: 330-7

    • NAID

      40016725993

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence, genomic organization and expression of two channel catfish, Ictalurus punctatus, ghrelin receptors.2009

    • Author(s)
      Small BC, Quiniou SM, Kaiya H.
    • Journal Title

      Comp Biochem Physiol A Mol Integr Physiol. 154(4)

      Pages: 451-64

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of growth hormone secretagogue-receptor and effect of quail ghrelin on gastrointestinal motility in Japanese quail.2009

    • Author(s)
      Kitazawa T, Maeda Y, Kaiya H.
    • Journal Title

      Regul Pept 158(1-3)

      Pages: 132-42

    • NAID

      120005761303

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of ghrelin in the house musk shrew (Suncus murinus) : cDNA cloning, peptide purification and tissue distribution.2009

    • Author(s)
      Ishida Y, Sakahara S, Tsutsui C, Kaiya H, Sakata I, Oda S, Sakai T.
    • Journal Title

      Peptides 30(5)

      Pages: 982-90

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin modulates fatty acid synthase and related transcription factor mRNA levels in a tissue-specific manner in neonatal broiler chicks.2009

    • Author(s)
      Buyse J, Janssen S, Geelissen S, Swennen Q, Kaiya H, Darras VM, Dridi S.
    • Journal Title

      Peptides 30(7)

      Pages: 1342-7

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin receptor (GHS-R)-like receptor and its genomic organisation in rainbow trout, Oncorhynchus mykiss.2009

    • Author(s)
      Kaiya H, Mori T, Miyazato M, Kangawa K.
    • Journal Title

      Comp Biochem Physiol A Mol Integr Physiol. 153(4)

      Pages: 438-50

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pre-and postprandial effects on ghrelin signaling in the brain and on the GH/IGF-I axis in the Mozambique tilapia (Oreochromis mossambicus).2009

    • Author(s)
      Peddu SC, Breves JP, Kaiya H, Gordon Grau E, Riley LG Jr.
    • Journal Title

      Gen Comp Endocrinol 161(3)

      Pages: 412-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and properties of ghrelin from the intestine of the goldfish, Carassius auratus.2009

    • Author(s)
      Miura T, Maruyama K, Kaiya H, Miyazato M, Kangawa K, Uchiyama M, Shioda S, Matsuda K.
    • Journal Title

      Peptides 30(4)

      Pages: 758-65

    • NAID

      10020188361

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Current knowledge of the roles of ghrelin in regulating food intake and energy balance in birds.2009

    • Author(s)
      Kaiya H, Furuse M, Miyazato M, Kangawa K.
    • Journal Title

      Gen Comp Endocrinol 163(1-2)

      Pages: 33-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin receptor(GHS-R)-like receptor and its genomic organisation in rainbowtrout, Oncorhynchus mykiss.2009

    • Author(s)
      Kaiya H, Mori T, Miyazato M, Kangawa K.
    • Journal Title

      Comparative Biochemistry and Physiology Part A 153

      Pages: 438-450

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning of growth hormone secretagogue-receptor and effect of quail ghrelin on gastrointestinal motility in Japanese quail.2009

    • Author(s)
      Kitazawa T, Maeda Y, Kaiya H.
    • Journal Title

      Regulatory Peptides 158

      Pages: 132-142

    • NAID

      120005761303

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and genomic sequence of a ghrelin receptor(GHS-R)-like receptor in the Mozambique tilapia, Oreochromis mossambicus.2009

    • Author(s)
      Kaiya H, Riley LG, Janzen W, Hirano T, Grau EG, Miyazato M, Kangawa K.
    • Journal Title

      Zoological Science 26

      Pages: 330-337

    • NAID

      40016725993

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Absence of effects of short-term fasting on plasma ghrelin and brain expression of ghrelin receptors in the tilapia, Oreochromis mossambicus.2008

    • Author(s)
      Riley LG, Fox BK, Breves JP, Kaiya H, Dorough CP, Hirano T, Grau EG.
    • Journal Title

      Zoolog Sci 25(8)

      Pages: 821-7

    • NAID

      40016386628

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin : a multifunctional hormone in non-mammalian vertebrates.2008

    • Author(s)
      Kaiya H, Miyazato M, Kangawa K, Peter RE, Unniappan S.
    • Journal Title

      Comp Biochem Physiol A Mol Integr Physiol. 149(2)

      Pages: 109-28

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghrelin : a multifunctional hormone in non-mammalian vertebrates2008

    • Author(s)
      Kaiya H, et al.
    • Journal Title

      Comparative Biochemistry and Physiology Part A 149

      Pages: 109-128

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Absence of effects of short-term fasting on plasma ghrelin and brain expression of ghrelin receptors in the tilapia, Oreochromis mossambicus2008

    • Author(s)
      Riley LG, et al.
    • Journal Title

      Zoological Science 25

      Pages: 821-827

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Current knowledge of the roles of ghrelin in regulating food intake and energy balance in birds2008

    • Author(s)
      Kaiya H, et al.
    • Journal Title

      General and Comparative Endocrinoology (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 無尾両生類2種のグレリン受容体(GHS-R1a)の同定2010

    • Author(s)
      海谷啓之、小泉泰士、今野紀文、内山実、寒川賢治、宮里幹也
    • Organizer
      第35回日本比較内分泌学会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] 無尾両生類2種のグレリン受容体(GHS-R1a)の同定2010

    • Author(s)
      海谷啓之、小泉泰士、今野紀文、内山実、寒川賢治、宮里幹也
    • Organizer
      日本比較内分泌学会
    • Place of Presentation
      静岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] キンギョグレリン受容体の同定とその性質2009

    • Author(s)
      海谷啓之、三浦徹、松田恒平、宮里幹也、寒川賢治
    • Organizer
      第34回日本比較内分泌学会
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report
  • [Presentation] Ghrelin receptor in goldfish : Identification and characterization2009

    • Author(s)
      Kaiya H, Miura T, Matsuda K, Miyazato M, Kangawa K.
    • Organizer
      16^<th> International Congress of Comparative Endocrinology
    • Place of Presentation
      中国(香港)
    • Related Report
      2010 Final Research Report
  • [Presentation] GHRELIN RECEPTORS IN GOLDFISH : IDENTIFICATION AND CHARACTERIZATION2009

    • Author(s)
      Kaiya H, Miura T, Matsuda K, Miyazato M, Kangawa K.
    • Organizer
      16th International Congress of Comparative Endocrinology
    • Place of Presentation
      香港(中国)
    • Related Report
      2009 Annual Research Report
  • [Presentation] グレリンにおいて脂肪酸修飾のある第3位のアミノ酸配列は全ての両生類でスレオニンなのか?2008

    • Author(s)
      海谷啓之、蓮沼至、筒井和義、寒川賢治、宮里幹也
    • Organizer
      第33回日本比較内分泌学会
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report
  • [Presentation] グレリンにおいて脂肪酸修飾のある第3位のアミノ酸は全ての両生類でスレオニンなのか?2008

    • Author(s)
      海谷啓之, 他
    • Organizer
      第33回日本比較内分泌学会大会
    • Place of Presentation
      広島
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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