| Project/Area Number |
20570065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
KAIYA Hiroyuki National Cardiovascular Center Research Institute, 生化学部, 室長 (40300975)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZATO Mikiya 独立行政法人国立循環器病研究センター, 生化学部, 部長 (50291183)
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| Co-Investigator(Renkei-kenkyūsha) |
KANGAWA Kenji 独立行政法人国立循環器病研究センター, 所長 (00112417)
MATSUDA Kouhei 富山大学, 理工学部, 教授 (60222303)
KITAZAWA Takio 酪農学園大学, 獣医学部, 教授 (50146338)
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| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | グレリン / グレリン受容体 / 魚類 / キンギョ / 受容体 / 脳 / 腸 / 内在性 / ペプチド / 精製 / GHS-R1a / GHS-R2a / 変異体 / ゲノム遺伝子 / 組織分布 / cDNA |
|---|
| Research Abstract |
In this study, we cloned cDNAs for four functional ghrelin receptors (GHS-R1a-1, 1a-2, 2a-1 and 2a-2) that composed of 360, 360, 366 and 367 amino acids, respectively in goldfish brain. Each receptor mRNA showed tissue-specific expression, suggesting that each receptor mediates tissue-specific action of ghrelin in goldfish. One species of ghrelin receptor, GHS-R1a has been known to be present in vertebrates other than fish examined so far. We tried to isolate new ligands for the new functional GHS-Ra, which is the novel type of ghrelin receptor, from tissue extracts of goldfish brain and intestine, but unfortunately the new ligand was not able to find out during the course of this study.
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