Structure, function and evolution of arginine kinase from Tetrahymena piriformis
Project/Area Number |
20570072
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Animal physiology/Animal behavior
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Research Institution | Kochi University |
Principal Investigator |
SUZUKI Tomohiko 高知大学, 教育研究部・自然科学系, 教授 (60145109)
|
Co-Investigator(Kenkyū-buntansha) |
UDA Kouji 高知大学, 教育研究部・自然科学系, 助教 (10448392)
|
Project Period (FY) |
2008 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 代謝生理 / 酵素 / 基質特異性 / アルギニンキナーゼ / テトラヒメナ / 進化 |
Research Abstract |
Two arginine kinases(AK1 and AK2) of Tetrahymena piriformis were characterized based on their structural and enzymatic properties. The AK2 had an unusual two-domain structure with two actives sites in a molecule. Intracellular localization of the two AKs was examined by using Western blotting and immunofluorescence technique, and the AK1 was identified unambiguously to localizing in cilia. On the other hand, AK2 appears to be distributed widely in cell body. We suggest that the localization of AK1 in cilia is promoted by putative myristoylated group of AK1.
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Report
(6 results)
Research Products
(43 results)
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[Journal Article] Structural basis for the mechanism and substrate specificity of glycocyamine kinase, a phosphagen kinase family member2010
Author(s)
Lim, K., Pullalarevu, S., Surabian, K. T., Howard, A., Suzuki, T., Moult, J., and Herzberg, O
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Journal Title
Biochemistry
Volume: 49
Pages: 2031-2041
Related Report
Peer Reviewed
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[Journal Article] Molecular characterization and kinetic properties of a novel two-domain taurocyamine kinase from the lung fluke Paragonimus westermani2009
Author(s)
Jarilla, B. R., Tokuhiro, S., Nagataki, M., Hong, S-J., Uda, K., Suzuki, T., and Agatsuma, T
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Journal Title
FEBS Lett
Volume: 583
Pages: 2218-2224
Related Report
Peer Reviewed
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[Journal Article] Evolution of the diverse array of phosphagen systems present in annelids2009
Author(s)
Suzuki, T., Uda, K., Adachi, M., Sanada, H., Tanaka, K., Mizuta, C., Ishida, K., and Ellington, W. R
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Journal Title
Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol
Volume: 152
Pages: 60-66
Related Report
Peer Reviewed
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[Journal Article] Evolution of the diverse array of phosphagen systems present in annelids.2009
Author(s)
Suzuki, T., Uda, K., Adachi, M., Sanada, H., Tanaka, K., Mizuta, C, Ishida, K., Ellington, W. R.
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Journal Title
Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol. 152
Pages: 60-66
Related Report
Peer Reviewed
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[Journal Article] Two-domain arginine kinase from the deep-sea clam Calyptogena kaikoi-Evidence of two active domains2008
Author(s)
Uda, K., Yamamoto, K., Iwasaki, I., Iwai, M., Fujikura, K., Ellington, W. R., and Suzuki, T
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Journal Title
Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol
Volume: 151
Pages: 176-182
NAID
Related Report
Peer Reviewed
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[Journal Article] Two-domain arginine kinase from the deep-sea clam Calyptogena kaikoi-Evidence of two active domains.2008
Author(s)
Uda, K., Yamamoto, K., Iwasaki, I., Iwai, M., Fujikura, K., Ellington, W. R., Suzuki, T.
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Journal Title
Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol. 151
Pages: 176-182
NAID
Related Report
Peer Reviewed
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