| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Two arginine kinases(AK1 and AK2) of Tetrahymena piriformis were characterized based on their structural and enzymatic properties. The AK2 had an unusual two-domain structure with two actives sites in a molecule. Intracellular localization of the two AKs was examined by using Western blotting and immunofluorescence technique, and the AK1 was identified unambiguously to localizing in cilia. On the other hand, AK2 appears to be distributed widely in cell body. We suggest that the localization of AK1 in cilia is promoted by putative myristoylated group of AK1.
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