Diapause control mechanisms by neurosecretory cells in insects

• Project/Area Number: 20570074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Osaka City University
• Principal Investigator: SHIGA Sakiko Osaka City University, 大学院・理学研究科, 教授 (90254383)
• Co-Investigator(Kenkyū-buntansha): NUMATA Hideharu 京都大学, 大学院・理学研究科, 教授 (70172749) ; GOTO Shinsuke 大阪市立大学, 大学院・理学研究科, 准教授 (70347483)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 休眠 / エクジステロイド / インスリン受容体 / 脳間部 / ルリキンバエ / 幼若ホルモン / 成虫休眠 / 脂肪体 / 神経分泌 / 昆虫 / 生理学

Research Abstract

Diapause controlling mechanisms by neurosecretory cells in the pars intercerebralis and pars lateralis were examined in the blow fly Protophormia terraenovae. FMRFamide, of which immunoreactivity was observed in the pars latelaris and the corpus allatum, showed week inhibitory effects on juvenile hormone production by the corpus allatum. Ecdysteroid titers and yolk protein gene expression were low under diapause conditions compared with nondiapause conditions. Removal experiments suggest the pars intercerebralis is important to control yolk protein expression but not ecdysteroid titers under nondiapause conditions

Research Products

• [Journal Article] Comparison of the effect of constant light on the circadian rhythm of white-eye mutant and wild-type blow fly Protophormia terraenovae. (2011)
  • Author(s): Hamasaka, Y., Watari, Y., Arai, T., Numata, H., Shiga, S.
  • Journal Title: Biological Rhythm Research in press
  • Peer Reviewed
• [Journal Article] Comparison of the effect of constant light on the circadian rhythm of white-eye mutant and wild-type blow fly Protophormia terraenovae. (2011)
  • Author(s): Hamasaka, Y
  • Journal Title: Biological Rhythm Research Volume: 42(In press)
  • Peer Reviewed
• [Journal Article] Effect of photoperiod on clock gene expression and subcellular distribution of PERIOD in the circadian clock neurons of the blow fly Protophormia terraenovae. (2010)
  • Author(s): Muguruma, F., Goto, S.G., Numata, H., Shiga, S.
  • Journal Title: Cell and Tissue Research 340 Pages: 497-507
  • Peer Reviewed
• [Journal Article] Mass spectrometic analysis of peptides in brain neurosecretory cells and neurohemal organs in the adult blow fly, Protophormia terraenovae. (2010)
  • Author(s): Inosaki, A., Yasuda, A., Shinada, T., Ohfune, Y., Numata, H., Shiga S.
  • Journal Title: Comparative Biochemistry and Physiology-Part A : Molecular & Integrative Physiology 155 Pages: 190-199
  • Peer Reviewed
• [Journal Article] Mass spectrometic analysis of peptides in brain neurosecretory cells and neurohemal organs in the adult blow fly, Protophormia terraenovae. (2010)
  • Author(s): Inosaki, A.
  • Journal Title: Comparative Biochemistry and Physiology - Part A : Molecular & Integrative Physiology 155巻 Pages: 190-199
  • Peer Reviewed
• [Journal Article] Effect of photoperiod on clock gene expression and subcellular distribution of PERIOD in the circadian clock neurons of the blow fly Protophormia terraenovae. (2010)
  • Author(s): Muguruma, F.
  • Journal Title: Cell and Tissue Research 340巻(In press)
  • Peer Reviewed
• [Journal Article] Morphological characterization of neurons projecting to the ring gland in the larval blow fly, Protophormia terraenovae. (2009)
  • Author(s): Hamanaka, Y., Tanaka, S., Numata, H., Shiga, S.
  • Journal Title: Zoological Science 26 Pages: 227-237
  • NAID: 40016615712
  • Peer Reviewed
• [Journal Article] Roles of PERIOD immunoreactive neurons in circadian rhythms and photoperiodism in the blow fly, Protophormia terraenovae. (2009)
  • Author(s): Shiga, S., Numata, H.
  • Journal Title: Journal of Experimental Biology 212 Pages: 867-877
  • Peer Reviewed
• [Journal Article] Roles of PERIOD immunoreactive neurons in circadian rhythms and photoperiodism in the blow fly, Protophormia terraenovae. (2009)
  • Author(s): Shiga, S.
  • Journal Title: Journal of Experimental Biology 212巻 Pages: 867-877
  • Peer Reviewed
• [Journal Article] Morphological characterization of neurons projecting to the ring gland in the larval blow fly, Protophormia terraenovae. (2009)
  • Author(s): Hamanaka, Y.
  • Journal Title: Zoological Science 26巻 Pages: 227-237
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Goto, S.G.
  • Journal Title: Photoperiodism in insects : perception of light and the role of clock genes. In : Photoperiodism : Seasonal Time Measurement. (Nelson, R.J., Denlinger, D.L. and Somers, D.(eds.))(Oxford University Press.) Pages: 258-286
• [Presentation] A neurobiological approach towards insect photoperiodism (2011)
  • Author(s): 志賀向子
  • Organizer: 9^<th> Gottingen Meeting of the German Neuroscience Society
  • Place of Presentation: Gottingen, Germany
  • Year and Date: 2011-03-27
• [Presentation] A neurobiological approach towards insect photoperiodism (2011)
  • Author(s): Sakiko Shiga
  • Organizer: Ninth Gottingen Meeting of the German Neuroscience Society
  • Place of Presentation: Gottingen, Germany(招待講演)
  • Year and Date: 2011-03-27
• [Presentation] ルリキンバエの脳間部神経分泌細胞による休民調節 (2010)
  • Author(s): 久我義行、志賀向子
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部(東京)
  • Year and Date: 2010-09-23
• [Presentation] ルリキンバエの脳間部神経分泌細胞による休眠調節 (2010)
  • Author(s): 久我義行
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部(文京区)
  • Year and Date: 2010-09-23
• [Presentation] ルリキンバエの成虫休眠における脳間部の役割 (2010)
  • Author(s): 久我義行、志賀向子
  • Organizer: 日本比較生理生化学会第32回大会
  • Place of Presentation: 九州産業大学工学部(福岡)
  • Year and Date: 2010-07-19
• [Presentation] ルリキンバエの成虫休眠における脳間部の役割 (2010)
  • Author(s): 久我義行
  • Organizer: 日本比較生理生化学会大32回大会
  • Place of Presentation: 九州産業大学工学部(福岡)
  • Year and Date: 2010-07-19
• [Presentation] ルリキンバエに脳内の時計遺伝子発現とPER分布に対する光周期の影響 (2009)
  • Author(s): 六車文明、後藤慎介、沼田英治、志賀向子
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター(豊中市)
  • Year and Date: 2009-10-23
• [Presentation] ルリキンバエ脳内の時計遺伝子発現とPER分布に対する光周期の影響 (2009)
  • Author(s): 六車文明
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会大31回大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター(豊中市)
  • Year and Date: 2009-10-23
• [Presentation] 体内時計を用いて季節を知る (2009)
  • Author(s): 志賀向子
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター(豊中市)
  • Year and Date: 2009-10-22
• [Presentation] 体内時計を用いて季節を知る (2009)
  • Author(s): 志賀向子
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会大31回大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター(豊中市)
  • Year and Date: 2009-10-22
• [Presentation] ルリキンバエにおける時計遺伝子の発現とPERIODの細胞内分布に対する光周期の影響 (2009)
  • Author(s): 六車文明、後藤慎介、沼田英治、志賀向子
  • Organizer: 日本動物学会第80回大会
  • Year and Date: 2009-09-17
• [Presentation] ルリキンバエにおける時計遺伝子の発現とPERIODの細胞内分布に対する光周期の影響 (2009)
  • Author(s): 六車文明
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡県コンベンションツアーセンターグランシップ(静岡市)
  • Year and Date: 2009-09-17
• [Presentation] ルリキンバエにおけるPERIODの遺伝子発現と細胞内局在に対する光周期の影響 (2008)
  • Author(s): 六車文明
  • Organizer: 第15回日本時間生物学会学術大会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2008-11-08
• [Presentation] ルリキンバエの時計機構に関わる神経回路 (2008)
  • Author(s): 志賀向子、浜中良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡大学(福岡市)
  • Year and Date: 2008-09-05
• [Presentation] ルリキンバエ卵黄タンパク質遺伝子の発現における脳間部の役割 (2008)
  • Author(s): 田中彩子、後藤慎介、沼田英治、志賀向子
  • Organizer: 日本比較生理生化学会第30回大会
  • Place of Presentation: 北海道大学(福岡市)
  • Year and Date: 2008-07-19
• [Presentation] ルリキンバエ卵黄タンパク質遺伝子の発現における脳間部の役割 (2008)
  • Author(s): 田中彩子
  • Organizer: 日本比較生理生化学会第30回大会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2008-07-09
• [Presentation] ルリキンバエにおけるPERIODの遺伝子発現と細胞内局在に対する光周期の影響 (2008)
  • Author(s): 六車文明、後藤慎介、沼田英治、志賀向子
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2008-01-18
• [Book] Photoperiodism : Seasonal Time Measurement.(Photoperiodism in insects : perception of light and the role of clock genes.)(Nelson, R.J., Denlinger, D.L. and Somers, D. (eds.)) (2009)
  • Author(s): Goto, S.G., Shiga, S., Numata, H.
  • Publisher: Oxford University Press