| Project/Area Number |
20570103
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
TONOZUKA Takashi Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (50285194)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIKWA Atsushi 東京農工大学, 大学院・農学研究院, 教授 (30218127)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | X線結晶解析 / 糖タンパク質糖鎖 / グルコシダーゼ / X線結晶構造析 / 大腸菌 / オリゴ糖 / マンノシダーゼ / X線結晶構造解析 / 黒麹菌 |
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| Research Abstract |
The purpose of this research was to elucidate the structure-function relationship of alpha-glucosidases and related enzymes, which act on the N-linked oligosaccharides. This research also investigated the relationship between the N-linked oligosaccharides and the protein structure. The three-dimensional structure of an enzyme homologous to processing alpha-glucosidase I has been determined, and the enzyme was shown to be useful for producing oligosaccharides. The expression system of a fungal processing alpha-glucosidase I was constructed, and the enzymatic properties were determined. We also investigated some enzymatic properties of a bacterial enzyme homologous to endo-alpha-1,2-mannosidase, an enzyme potentially physiologically related to processing alpha-glucosidases. This was the first report on the enzymatic characterization of a bacterial endo-alpha-1,2-mannosidase.
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