Oligomerization dynamics on cytoskeletal proteins using synchrotron high-pressure jump small-angle scattering technique.

• Project/Area Number: 20570106
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Gifu University
• Principal Investigator: FUJISAWA Tetsuro Gifu University, 工学部, 教授 (10231565)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: フォールディング / 小角散乱 / 高圧 / タンパク質 / ダイナミクス / 放射光 / 細胞骨格タンパク質 / かい離・会合 / 電気泳動

Research Abstract

We improved our own designed synchrotron high-pressure jump small-angle scattering system, by which stabilities on various cytoskeletal fibrous proteins were explored. Actin homologue proteins, ParM, FtsZ, Alfa, MreB were studied, and the result were published in three papres. We also applied the high-pressure system to lysozyme amyloid filament.

Research Products

• [Journal Article] Time-resolved small-angle X-ray scattering study of the folding dynamics of barnase. (2011)
  • Author(s): Konuma, T., Kimura, T., Matsumoto, S.Goto, Y., Fujisawa, T., Fersht, AR., Takahashi, S.
  • Journal Title: Journal of Molecular Biology Volume: 405 Pages: 1284-1294
  • Peer Reviewed
• [Journal Article] 高静水圧下におけるX線小角散乱解析 (2010)
  • Author(s): 藤澤哲郎
  • Journal Title: 化学と生物 48 Pages: 188-194
  • NAID: 10026437884
• [Journal Article] タンパク質の小角散乱法による低分解能構造解析 (2010)
  • Author(s): 藤澤哲郎
  • Journal Title: 生化学 82 Pages: 122-126
  • NAID: 10026300488
• [Journal Article] Filament structure, organization, and dynamics in MreB sheets. (2010)
  • Author(s): Popp D, Narita A, Fujisawa T, Maeda Y, Robinson RC.
  • Journal Title: J.Biol.Chem. 285 Pages: 15858-15865
  • Peer Reviewed
• [Journal Article] Polymeric structures and dynamic properties of the bacterial actin AlfA. (2010)
  • Author(s): Popp D, Narita A, Fujisawa T, Robinson RC.
  • Journal Title: J.Mol.Biol. 397 Pages: 1031-1041
  • Peer Reviewed
• [Journal Article] Structural properties of silkworm small heat-shock proteins : sHSP19.9 and sHSP20.8. (2010)
  • Author(s): Hossain T, Teshiba S, Fujisawa T, Banno Y, Aso Y.
  • Journal Title: Biosci.Biotechnol.Biochem. 74 Pages: 1556-1563
  • Peer Reviewed
• [Journal Article] Polymeric structures and dynamic properties of the bacterial actin AlfA. (2010)
  • Author(s): Popp, D.,Narita, A., Ghoshdastider, U., Maeda, K.,Maeda, Y., Oda, T., Fujisawa, T., Onishi, H., Ito, K., Robinson, R.C.
  • Journal Title: Journal of Molecular Biology Volume: 397 Pages: 1031-1041
  • Peer Reviewed
• [Journal Article] Filament structure, organization and dynamics in MreB sheets. (2010)
  • Author(s): Popp, D., Narita, A., Maeda, K., Fujisawa, T., Ghoshdastider, U., Iwasa, M., Maeda, Y., Robinson, R.C.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 15858-15865
  • Peer Reviewed
• [Journal Article] Structural properties of silkworm small heat-shock proteins : sHSP19.9 and sHSP20.8. (2010)
  • Author(s): Hossain, T.,Teshiba, S., Shigeoka, Y., Fujisawa, T., Inoko, Y., Sakano, D., Yamamoto, K., Banno, Y., Aso, Y.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: 74 Pages: 1556-1563
  • Peer Reviewed
• [Journal Article] 分子生物学におけるX線・中性子小角散乱法のほかの構造解析手法との相補性 (2009)
  • Author(s): 藤澤哲郎
  • Journal Title: 日本結晶学会誌 51 Pages: 91-93
• [Journal Article] Amyloid fibrillation rate differs greatly among single-disulfide variants of hen lysozyme (2009)
  • Author(s): Kono R、Fujisawa T、Akasaka K、Tachibana H
  • Journal Title: J.Biol.Macromol. 9 Pages: 23-30
  • Peer Reviewed
• [Journal Article] Amyloid fibrillation rate differs greatly among single-disulfide variants of hen lysozyme (2009)
  • Author(s): Kono, R., Fujisawa, T., Akasaka, K., Tachibane, H.
  • Journal Title: J.Biol.Macromol. 9 Pages: 23-30
  • Peer Reviewed
• [Journal Article] Molecular structure of the ParM polymer and the mechanism leading to its nucleotide-driven dynamic instability (2008)
  • Author(s): Popp, D., Narita, A., Fujisawa T, Onishi, H., Maeda, Y.
  • Journal Title: EMBO J. 27 Pages: 570-579
  • Peer Reviewed
• [Journal Article] Molecular structure of the ParM polymer and the mechanlsm leadlng to its nucleotide-drlven dynamic instability (2008)
  • Author(s): Popp, D., Narlta, A., Oda, T., Fujlsawa, T., Matsuo, H., Nitanal, Y., lwasa, M., Maeda, K., Onishi. H.
  • Journal Title: EMBO J. 27 Pages: 570-579
  • Peer Reviewed
• [Journal Article] RecR forms a ring-like tetramer that enclrcles dsDNA by formlng a complex with RecF. (2008)
  • Author(s): Honda, M., Fujisawa, T., Shibata, T., Mikawa, T.
  • Journal Title: Nuclelc Acids Res. 36 Pages: 5013-5020
  • Peer Reviewed
• [Journal Article] Stereocomplex Formatlon through Reorganizatlon of Poly(L-lactic acld) and Poly (D-lactic acld) Crystals (2008)
  • Author(s): Fujita, M., Sawayanagi, T., Abe, H., Tanaka, T., lwata, T., lto, K., Fujisawa, T., Maeda, M.
  • Journal Title: Macromolecules 41 Pages: 2852-2858
  • Peer Reviewed
• [Presentation] アクチン相同骨格タンパク質フィラメントMreB、Alfaの高圧X線小角散乱による研究 (2010)
  • Author(s): 藤澤哲郎、石黒亮、松尾博史、David Popp
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] 高静水圧電気泳動法によるリゾチーム変異体アミロイド原線維のかい離平衡の研究 (2010)
  • Author(s): 石黒亮、松尾博史、橘秀樹、藤澤哲郎
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] 中性子小角散乱法による大腸菌外膜蛋白質OmpA界面活性剤複合体の構造解析 (2010)
  • Author(s): 小野内優理、藤澤哲郎、石黒亮、亀山啓一
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] 中性子小角散乱法による大腸菌外膜蛋白質OmpA界面活性剤複合体の構造解析 (2010)
  • Author(s): 小野内優理、藤澤哲郎、石黒亮、亀山啓一
  • Organizer: 日本生化学会中部支部例会
  • Place of Presentation: 名古屋大学医学部
  • Year and Date: 2010-05-29
• [Presentation] 高圧ジャンプX線小角散乱法を用いた細胞骨格フィラメントの安定性とヌクレオチドキャップの研究 (2008)
  • Author(s): Fujisawa T., Matsuo H., Iwasa, M., Erickson, H.P., Kamimura, S., Maeda, Y., Popp, D.
  • Year and Date: 2008-12-04
• [Presentation] Nucleotide caps and the stabllity of cytoskeletal fibrous protelns : A high-pressure small-angle x-ray scattering study (2008)
  • Author(s): Fujlsawa, T., Matsuo, H., Iwasa, M., Erlckson, H. P., Kamlmura, S., Maeda, Y., Popp, D.
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Book] 生体系、ソフトマターの分光 査読無、「X線・放射光の分光」 (2009)
  • Author(s): 藤澤哲郎
  • Publisher: 日本分光学会編講談社
• [Book] X線・放射光の分光 (2009)
  • Author(s): 藤澤哲郎、丑田公規編蓍
  • Total Pages: 23
  • Publisher: 講談社
• [Remarks] ホームページ等
  • URL: http://www1.gifu-u.ac.jp/~fujilab/
• [Remarks]
  • URL: http://www1.gifu-u.ac.jp/~fujilab/
• [Remarks]
  • URL: http://biomol.gifu-u.ac.jp/~bbc2/