Oligomerization dynamics on cytoskeletal proteins using synchrotron high-pressure jump small-angle scattering technique.
Project/Area Number |
20570106
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Gifu University |
Principal Investigator |
FUJISAWA Tetsuro Gifu University, 工学部, 教授 (10231565)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | フォールディング / 小角散乱 / 高圧 / タンパク質 / ダイナミクス / 放射光 / 細胞骨格タンパク質 / かい離・会合 / 電気泳動 |
Research Abstract |
We improved our own designed synchrotron high-pressure jump small-angle scattering system, by which stabilities on various cytoskeletal fibrous proteins were explored. Actin homologue proteins, ParM, FtsZ, Alfa, MreB were studied, and the result were published in three papres. We also applied the high-pressure system to lysozyme amyloid filament.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Time-resolved small-angle X-ray scattering study of the folding dynamics of barnase.2011
Author(s)
Konuma, T., Kimura, T., Matsumoto, S.Goto, Y., Fujisawa, T., Fersht, AR., Takahashi, S.
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Journal Title
Journal of Molecular Biology
Volume: 405
Pages: 1284-1294
Related Report
Peer Reviewed
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[Journal Article] Polymeric structures and dynamic properties of the bacterial actin AlfA.2010
Author(s)
Popp, D.,Narita, A., Ghoshdastider, U., Maeda, K.,Maeda, Y., Oda, T., Fujisawa, T., Onishi, H., Ito, K., Robinson, R.C.
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Journal Title
Journal of Molecular Biology
Volume: 397
Pages: 1031-1041
Related Report
Peer Reviewed
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[Journal Article] Filament structure, organization and dynamics in MreB sheets.2010
Author(s)
Popp, D., Narita, A., Maeda, K., Fujisawa, T., Ghoshdastider, U., Iwasa, M., Maeda, Y., Robinson, R.C.
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Journal Title
Journal of Biological Chemistry
Volume: 285
Pages: 15858-15865
Related Report
Peer Reviewed
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[Journal Article] Structural properties of silkworm small heat-shock proteins : sHSP19.9 and sHSP20.8.2010
Author(s)
Hossain, T.,Teshiba, S., Shigeoka, Y., Fujisawa, T., Inoko, Y., Sakano, D., Yamamoto, K., Banno, Y., Aso, Y.
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Journal Title
Bioscience, Biotechnology and Biochemistry
Volume: 74
Pages: 1556-1563
Related Report
Peer Reviewed
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[Journal Article] Molecular structure of the ParM polymer and the mechanlsm leadlng to its nucleotide-drlven dynamic instability2008
Author(s)
Popp, D., Narlta, A., Oda, T., Fujlsawa, T., Matsuo, H., Nitanal, Y., lwasa, M., Maeda, K., Onishi. H.
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Journal Title
EMBO J. 27
Pages: 570-579
Related Report
Peer Reviewed
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[Journal Article] Stereocomplex Formatlon through Reorganizatlon of Poly(L-lactic acld) and Poly (D-lactic acld) Crystals2008
Author(s)
Fujita, M., Sawayanagi, T., Abe, H., Tanaka, T., lwata, T., lto, K., Fujisawa, T., Maeda, M.
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Journal Title
Macromolecules 41
Pages: 2852-2858
Related Report
Peer Reviewed
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[Presentation] 高圧ジャンプX線小角散乱法を用いた細胞骨格フィラメントの安定性とヌクレオチドキャップの研究2008
Author(s)
Fujisawa T., Matsuo H., Iwasa, M., Erickson, H.P., Kamimura, S., Maeda, Y., Popp, D.
Year and Date
2008-12-04
Related Report
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[Presentation] Nucleotide caps and the stabllity of cytoskeletal fibrous protelns : A high-pressure small-angle x-ray scattering study2008
Author(s)
Fujlsawa, T., Matsuo, H., Iwasa, M., Erlckson, H. P., Kamlmura, S., Maeda, Y., Popp, D.
Organizer
第46回日本生物物理学会年会
Place of Presentation
福岡
Year and Date
2008-12-04
Related Report
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