Structural change of telomere DNA induced by radical reaction

• Project/Area Number: 20570108
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KANAORI Kenji Kyoto Institute of Technology, 工芸科学研究科, 准教授 (30273543)
• Research Collaborator: TAJIMA Kunihiko 京都工芸繊維大学, 工芸科学研究科, 教授 (50163457)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ラジカル / 核酸 / 損傷 / NMR / ESR

Research Abstract

Telomere that exists in eukaryote's chromosome terminus is composed of telomeric DNA with the repetitive sequence to which the guanine base is abundant and of telomere binding proteins. The damage of the telomere sequence was verified by researching this telomeric site by using stopped-flow-ESR and NMR and generating superoxide anion radical and singlet oxygen. In addition, telomere DNA damage with the nitrous acid was concurrently studied, and the cross-linking reaction with the protein was also examined.

Research Products

• [Journal Article] UV luminescent organic-capped ZnO quantum dots synthesized by alkoxide hydrolysis with dilute water (2011)
  • Author(s): T.Omata, K.Takahashi, S.Hashimoto, Y.Maeda, K.Nose, S.Otsuka-Yao-Matsuo, K.Kanaori
  • Journal Title: J.Colloid. Interface Sci. 355 Pages: 274-281
  • Peer Reviewed
• [Journal Article] UV luminescent organic-capped ZnO quantum dots synthesized by alkoxide hydrolysis with dilute water (2011)
  • Author(s): T.Omata
  • Journal Title: J.Colloid.Interface Sci. Volume: 355 Pages: 274-281
  • Peer Reviewed
• [Journal Article] ESR spectrum attributed to trisulfide Neutral Radical[RSS(R)S.R]of protein observed for a-keratin present in white human hair (2010)
  • Author(s): N.Yanagi, M.Niwa, Y.Sakurai, A.Nakajima, K.Kanaori, K.Tajima
  • Journal Title: Chem.Lett. 39 Pages: 756-757
  • Peer Reviewed
• [Journal Article] ESR study of disulfide neutral radical of keratin present in dried white human hair exposed to near-UV radiation (2010)
  • Author(s): N.Yanagi, M.Niwa, Y.Sakurai, A.Nakajima, K.Kanaori, K.Tajima
  • Journal Title: Chem.Lett. 39 Pages: 340-341
  • Peer Reviewed
• [Journal Article] Creation of a type 1 blue copper site within a de novo coiled-coil protein scaffold (2010)
  • Author(s): D.Shiga, D.Nakane, T.Inomata, Y.Funahashi, H.Masuda, A.Kikuchi, M.Oda, M.Noda, S.Uchiyama, K.Fukui, K.Kanaori, K.Tajima, Y.Takano, H.Nakamura, T.Tanaka
  • Journal Title: J.Am.Chem.Soc. 132 Pages: 18191-18198
  • Peer Reviewed
• [Journal Article] Creation of a type 1 blue copper site within a de novo coiled-coil protein scaffold (2010)
  • Author(s): D.Shiga
  • Journal Title: J.Am.Chem.Soc. Volume: 132 Pages: 18191-18198
  • Peer Reviewed
• [Journal Article] ESR spectrum attributed to trisulfide neutral radical [RSS(R)S・R] of protein observed for α-keratin present in white human hair (2010)
  • Author(s): N.Yanagi
  • Journal Title: Chem.Lett. Volume: 39 Pages: 756-757
  • Peer Reviewed
• [Journal Article] ESR study of disulfide neutral radical of α-Keratin present in dried white human hair exposed to near-UV radiation (2010)
  • Author(s): N.Yanagi
  • Journal Title: Chemistry Letters 38 Pages: 340-341
  • Peer Reviewed
• [Journal Article] Effect of thermal treatment on the photocatalytic degradation of ethylene, trichloroethylene (2009)
  • Author(s): S.Yamazaki, H.Abe, T.Tanimura, Y.Yamasaki, K.Kanaori, K.Tajima
  • Journal Title: Res.Chem.Intermed. 35 Pages: 91-101
  • Peer Reviewed
• [Journal Article] Development of a HPLC-ESR spin-trapping system for post-column on-line detection of superoxide radical scavenging ability of column eluates (2009)
  • Author(s): R.-K.Watanabe, C.Morimoto, S.Sakamoto, A.Nakajima, K.Kanaori, K.Tajima
  • Journal Title: Chem.Lett. 39 Pages: 340-341
  • NAID: 10025123787
  • Peer Reviewed
• [Journal Article] Identification of an antiamyloidogenic substance from mulberry leaves (2009)
  • Author(s): P.Khaengkhan
  • Journal Title: Neurochemistry 20 Pages: 1214-1218
  • Peer Reviewed
• [Journal Article] Development of a HPLC-ESR spin-trapping system for post-colum on-line detection of superoxide radical scavenging ability of column eluates (2009)
  • Author(s): Y.Sakurai
  • Journal Title: Chemistry Letters 38 Pages: 1270-1271
  • Peer Reviewed
• [Journal Article] Development of a HPLC-ESR spin-trapping system for post-colum on-line detection of superoxide radical scavenging ability of column eluates (2009)
  • Author(s): Y. Sakurai
  • Journal Title: Chemistry Letters 38(in press)
  • Peer Reviewed
• [Journal Article] Kinetic investigation of reaction of ascorbate and hydroxyl radical adduct of DMPO(5,5-dimethyl-1-pyrroline N-Oxide) studied by stopped-flow ESR (2008)
  • Author(s): Y.Sakurai, H.Sanuki, R.K.-.Watanabe, T.Ideguchi, N.Yanagi, K.Kawai, K.Kanaori, K.Tajima
  • Journal Title: Chem.Lett. 37 Pages: 1270-1271
  • NAID: 10024398217
  • Peer Reviewed
• [Journal Article] Quantitative spin-trapping ESR investigation on reaction of hydroxyl radical and selected scavengers by a newly developed flow-injection ESR System (2008)
  • Author(s): R.-K.Watanabe, Y.Sakurai, C.Morimoto, S.Sakamoto, K.Kanaori, K.Tajima
  • Journal Title: Chem.Lett. 37 Pages: 612-613
  • NAID: 10021081168
  • Peer Reviewed
• [Journal Article] Kinetic investigation of reaction of ascobate and hydroxyl radical adduct of DMPO (5, 5-dimethyl-1-pyrroline N-oxide)studied by stopped-flow ESR (2008)
  • Author(s): Y. Sakurai
  • Journal Title: Chemistry Letters 37 Pages: 1270-1271
  • Peer Reviewed
• [Journal Article] Quantitative spin-trapping ESR investigation on reaction of hydroxyl radical and selected scavengers by a newly developed flow-injection ESR system (2008)
  • Author(s): R. K. Watanabe
  • Journal Title: Chemistry Letters 37 Pages: 612-613
  • Peer Reviewed
• [Presentation] テロメア四重鎖におけるグアニン塩基の損傷反応 (2011)
  • Author(s): 金折賢二、能勢健史
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-27
• [Presentation] オキザノシン塩基とシステインとの反応 (2011)
  • Author(s): 金折賢二、小池洋平
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-27
• [Presentation] Applications of Flow-ESR Technique for Mechanistic Study of Biological Radical Reaction (2010)
  • Author(s): 田嶋邦彦、金折賢二
  • Organizer: 7th Asia-Pacific EPR/ESR Symposium
  • Place of Presentation: 大韓民国、済州島国際コンベンションセンター(招待講演)
  • Year and Date: 2010-10-13
• [Presentation] T quaternary state of oxy haemoglobin in the presence of heterotropic effectors (2010)
  • Author(s): 金折賢二、田尻祐介
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] HPLC-ESR法によるフェノール誘導体およびコーヒーの無機ニトロキシドラジカル(NDS, nitrosodisulfonate)消去活性のオンライン評価 (2010)
  • Author(s): 櫻井康博、金折賢二、田嶋邦彦
  • Organizer: 第57回日本食品科学工学会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2010-09-03
• [Presentation] Flow-injection-spin-trapping-ESR法によるスーパーオキシドラジカルとフェノール性抗酸化物質の二次反応速度定数の解析 (2010)
  • Author(s): 櫻井康博、金折賢二、田嶋邦彦
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 神奈川県県民ホール
  • Year and Date: 2010-06-24
• [Presentation] Flow-Injection ESRによるスーパーオキシドラジカルと生体関連分子の2次反応速度定数の評価 (2010)
  • Author(s): 櫻井康博、金折賢二、田嶋邦彦
  • Organizer: 日本化学会第90春期年会
  • Place of Presentation: 近畿大
  • Year and Date: 2010-03-26
• [Presentation] ジスルフィドラジカルを経由するα-ケラチン-SS結合の光酸化反応機構のESR研究 (2009)
  • Author(s): 柳直樹、金折賢二、田嶋邦彦
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-30
• [Presentation] ジスルフィドラジカルを経由するα-ケラチン-ss結合の光酸化反応機構のESR研究 (2009)
  • Author(s): 柳直樹
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-30
• [Presentation] テロメア四重鎖におけるグアニン塩基の脱アミノ化反応 (2009)
  • Author(s): 金折賢二、梅木慎吾
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-29
• [Presentation] オキザニン:チミン塩基対を含むDNA二重鎖の構造 (2009)
  • Author(s): 金折賢二、森本寛久
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-29
• [Presentation] テロメア四重鎖におけるグアニン塩基の脱アミノイヒ反応 (2009)
  • Author(s): 梅木慎吾
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-29
• [Presentation] オキザニン : チミン塩基対を含むDNA二重鎖の構造 (2009)
  • Author(s): 森本寛久
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-29
• [Presentation] Flow-Injection ESRによるヒドロキシルラジカルとアミノ酸の2次反応速度の評価と構造活性相関 (2009)
  • Author(s): 櫻井康博、金折賢二、田嶋邦彦
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-27
• [Presentation] ストップトフローESR法によるフラボノイド由来セミキノンラジカルの生成消失機構解析 (2009)
  • Author(s): 佐貫穂高、金折賢二、田嶋邦彦
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-27
• [Presentation] ストツプトフローESR法によるフラボノイド由来セミキノンラジカルの生成消失機構解析 (2009)
  • Author(s): 佐貫穂高
  • Organizer: 日本化学会第89春期年会
  • Place of Presentation: 船橋日大
  • Year and Date: 2009-03-27