Signaling-molecular mechanism of a novel microglia EF-hand protein Iba1 deduced from X-ray structures
| Project/Area Number |
20570109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
KAMITORI Shigehiro Kagawa University, 総合生命科学研究センター, 教授 (00262246)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Hiromi 香川大学, 総合生命科学研究センター, 准教授 (10313305)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | X線構造 / シグナル伝達 / カルシウム結合タンパク質 / EFハンドタンパク質多量体形成 / ミクログリア / カルシウム結合蛋白質 / EFハンド蛋白質 / 多量体形成 / 変異蛋白質 / 動的光散乱 / X線結晶解析 / EFハンドタンパク質 / アクチン結合タンパク質 / タンパク質複合体 / タンデムリピート |
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| Research Abstract |
Iba1 with 147 amino acid residues has been identified as a novel calcium-binding EF-hand protein, expressed in microglia/macrophages, and is expected to be a key factor in the activating of microglia. To elucidate signaling-molecular mechanism of Iba1, X-ray structural analysis and dynamic light scattering experiment of Iba1 were done. The results showed that Iba1 exists as a dimer in solution, and that Iba1 changes its oligomer state from dimer to monomer, depending on the hydrophobic environment.
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Report
(4 results)
Research Products
(12 results)
