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Golgi structure and golgins

Research Project

Project/Area Number 20570114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionFukuoka University

Principal Investigator

MISUMI Yoshio  Fukuoka University, 医学部, 准教授 (10148877)

Co-Investigator(Renkei-kenkyūsha) SOHDA Miwa  新潟大学, 医歯学系, 助教 (20258528)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords細胞小器官 / ゴルジ体 / 小胞輸送 / golgin / 局在化 / golain
Research Abstract

The coiled-coil Golgi membrane protein golgin-84 functions as a tethering factor for COPI vesicles. Protein interaction analyses have revealed that golgin-84 interacts with another tether, the conserved oligomeric Golgi (COG) complex, through its subunit Cog7. Therefore, we explored the function of golgin-84 as the tether for COPI vesicles of intra-Golgi retrograde traffic. First, glycosylic maturation of both plasma membrane (CD44) and lysosomal (lampl) glycoproteins was distorted in golgin-84 knockdown (KD) cells. The depletion of golgin-84 caused fragmentation of the Golgi with the mislocalization of Golgi resident proteins, resulting in the accumulation of vesicles carrying intra-Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and cis-Golgi membrane protein GPP130. Similar observations were obtained by diminution of the COG complex, suggesting a strong correlation between the two tethers. Indeed, COG complex-dependent (CCD) vesicles that accumulate in Cog3 or Cog7 KD cells carried golgin-84. Surprisingly, the interaction between golgin-84 and another candidate tethering partner CASP (CDP/cut alternatively spliced product) decreased in Cog3 KD cells. These results indicate that golgin-84 on COPI vesicles interact with the COG complex before SNARE assembly, suggesting that the interaction of golgin-84 with COG plays an important role in the tethering process of intra-Golgi retrograde vesicle traffic.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009 2008 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] The novel cis-acting element GASE regulates transcriptional induction by the Golgi stress response.2011

    • Author(s)
      Oku M, Tanakura S, Uemura A, Sohda M, Misumi Y, Taniguchi M, Wakabayashi S, Yoshida H
    • Journal Title

      Cell Struct Funct 36(1)

      Pages: 1-12

    • NAID

      130004137558

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The novel cis-acting element GASE regulates transcriptional induction by the Golgi stress response.2011

    • Author(s)
      Oku, M
    • Journal Title

      Cell Struct Funct

      Volume: 36 Pages: 1-12

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein2010

    • Author(s)
      Fujita A, Misumi Y
    • Journal Title

      Mol Biol Rep Nov.24

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of golgin-84 with the COG complex mediates the intra-Golgi retrograde transport.2010

    • Author(s)
      Sohda M, Misumi Y, Yamamoto A, Nakamura N, Ogata S, Sakisaka S, Hirose S, Ikehara Y, Oda K
    • Journal Title

      TrafFic 11

      Pages: 1552-1566

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of golgin-84 with the COG complex mediates the intra-Golgi retrograde transport.2010

    • Author(s)
      Sohda, M
    • Journal Title

      Traffic

      Volume: 11 Pages: 1552-1566

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fission yeast ucp3 gene encodes a putative Arf6 GTPase-activating protein.2010

    • Author(s)
      Fujita, A.
    • Journal Title

      Mol Biol Rep

      Volume: Nov24(On line only)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fissionyeast syt22protein, a putative Arfguanine nucleotide exchange factor, is necessary for newend take off.2009

    • Author(s)
      Fujita A, Misumi Y
    • Journal Title

      FEMS Microbiol Lett 294

      Pages: 191-197

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fissionyeast syt22protein, a putative Arf guanine nucleotide exchange factor, is necessary for newend take off2009

    • Author(s)
      Fujita, A
    • Journal Title

      FEMS Microbiol Lett 294

      Pages: 191-197

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] YIPFS and YIFIArecycle between the ER and the Golgi apparatus and are involved in the maintenance of the Golgi structure.2008

    • Author(s)
      Yoshida Y, Suzuki K, Yamamoto A, Sakai N, Bando M, Tanimoto K, Yamaguchi Y, Sakaguchi T, Akhter H, Fujii G, Yoshimura SI, Ogata S, Sohda M, Misumi Y, Nakamura N
    • Journal Title

      Exp Cell Res 314(19)

      Pages: 3427-3443

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Kv4 accessory protein DPPX is a critical regulator of membrane excitability in hippocampal CAl pyramidal neurons.2008

    • Author(s)
      Kim J, Nadal M, Clemens AM, Baron M, Jung SC, Misumi Y, Rudy B, Hoffman DA.J
    • Journal Title

      Neurophysiol 100(4)

      Pages: 2834-1847

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406.2008

    • Author(s)
      Numa N, Ishida Y, Nasu M, Sohda M, Misumi Y, Noda T, Oda K
    • Journal Title

      FEBS J 275(11)

      Pages: 2727-2737

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 4062008

    • Author(s)
      Numa, N
    • Journal Title

      FEBS J. 275(11)

      Pages: 2727-2737

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Kv4 accessory protein DPPX is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons.2008

    • Author(s)
      Kim, J
    • Journal Title

      J Neurophysiol 100(4)

      Pages: 1834-1847

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] YIPF5 and YIF1A recycle between the ER and the Golgi apparatus and are involved in the maintenance of the Golgi structure2008

    • Author(s)
      Yoshida, Y
    • Journal Title

      Exp Cell Res 314(19)

      Pages: 3427-3443

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Kimimitsu Oda Interaction of golgin-84 with the conserved oligomeric Golgi(COG) complex mediates the intra-Golgi retrograde transport2010

    • Author(s)
      Miwa Sohda, Yshio Misumi, Akitsugu Yamamoto, Nobuhiro Nakamura
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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