STRUCTURE-FUNCTION RELATIONSHIP OF CHOLESTEROL-ENRICHED MEMBRANE MICRODOMAINS
| Project/Area Number |
20570116
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
IWASHITA Yoshiko Tokyo Metropolitan Institute of Gerontology, 薬学部, 客員研究員 (50111498)
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| Co-Investigator(Kenkyū-buntansha) |
IWASHITA Shintaro いわき明星大学, 薬学部, 教授 (90092147)
INOMATA Mitsushi 地方独立行政法人東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究員 (30142649)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 生体膜 / Tリンパ球 / 膜機能ドメイン / コレステロール / シグナル伝達 / 脂質ラフト / 細胞膜 / 脂質ラフ |
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| Research Abstract |
To study the structure-function relationship of membrane microdomains (lipid rafts) involved in signal transduction, we examined the alterations of lipid rafts in an age-associated decline in T-cell receptor signaling. By analyzing resting and stimulated mouse CD4^+ T cells, we found that the following alterations may be related to the age-dependent decline in T-cell receptor signaling : 1) age-dependent alterations in several lipid raft-associated molecules, and resulting dysregulation of a negative SFK (Src family kinase) regulatory system ; 2) age-associated up-regulation of PD-1-positive T cells that exhibit proliferative hyporesponsiveness; 3) altered localization/topology of cholesterol, one of major constituents of lipid rafts.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Use of dansyl-cholestanol as a probe of cholesterol behavior in membranes of living cells.2010
Author(s)
Huang, H., McIntosh, A.L., Atshaves, B.P., Ohno-Iwashita Y., Kier, A.B., Schroeder, F.
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Journal Title
J.Lipid Res. 51
Pages: 1157-1172
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Peer Reviewed
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[Journal Article]2008
Author(s)
McInstosh, A. L., et al.
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Journal Title
Probes and Tags to Study Biomolecular Function : for proteins, RNA, and Membranes(Wiley-VCH)
Pages: 1-33
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[Presentation] Cholesterol gradient in biological membranes2008
Author(s)
Murase-Tamada, K., Murate, M., Hayakawa, T., Nakamura, K., Ito, K., Shimada Y., Ohno-Iwashita Y., Kobayashi, T.
Organizer
International Symposium on Molecular System Life Sciences
Place of Presentation
Kobe, Japan
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[Book] Cholesterol-Binding Toxins Anti-cholesterol Antibodies as Structural Probes for Cholesterol Localization. Vol.51, p.597-621(Harris, J.R.ed.)(Cholesterol Binding Cholesterol Transport Proteins)(Chapter 22) Seies : Subcellular Biochemistry2010
Author(s)
Ohno-Iwashita Y., Shimada, Y., Hayashi, M. Iwamoto, M., Iwashita, S., Inomata, M.
Publisher
Springer
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[Book] Fluorescent sterols to study cholesterol trafficking in living cells. P.1-33(Miller, L.W.ed.)(Probes Tags to Study Biomolecular Function : for Proteins, RNA, Membranes)2008
Author(s)
McIntosh, A.L., Huang, H., Atshaves, B.P., Storey, S.M., Gal legos, A.M., Spencer, T.A., Bittman, R., Ohno-Iwashita, Y., Kier, A.B., Schroeder, F.
Publisher
Wiley-VCH, Weinheim
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