Sequence-specificity and binding-cooperativity of multiple DNA-binding domains of CUT-homeodomain transcription factors

• Project/Area Number: 20570119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YAMASAKI Kazuhiko National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 主任研究員 (00358243)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DNA結合タンパク質 / 協同性と競合性 / 転写制御 / 免疫細胞分化 / NMR分光解析 / 等温滴定熱量分析 / 認識コンセンサス配列 / X線結晶解析 / 表面プラズモン共鳴

Research Abstract

The DNA-binding mechanism by multiple DNA-binding domains of CUT-homeodomain transcription factors were analyzed by methods of structural biology and physical chemistry. NMR and isothermal titration calorimetry analyses revealed that CUTr1 domain and homeodomain of transcription factor SATB1 bind to DNA containing TAATA sequence in a cooperative manner. A SELEX experiment showed that CUTr1 has the major role in determining the sequence specificity.

Research Products

• [Journal Article] The combination of sequence-specific and nonspecific binding modes of transcription factor SATB1 (2016)
  • Author(s): Kazuhiko Yamasaki and Tomoko Yamasaki
  • Journal Title: Biochemical Journal Volume: 473 Issue: 19 Pages: 3321-3339
  • DOI: 10.1042/bcj20160236
  • Peer Reviewed
• [Presentation] 転写因子SATBIのDNA認識における弱い配列特異的結合と強い非特異的結合の組み合わせ (2014)
  • Author(s): 山崎 和彦, 山崎 智子
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-16
• [Presentation] 転写因子SATBIの複数のDNA結合ドメインによるDNA, 認識のNMR解析 (2008)
  • Author(s): 山崎和彦, 山崎智子
  • Organizer: BMB2008
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 転写因子SATB1の複数のDNA結合ドメインによるDNA認識のNMR解析 (2008)
  • Author(s): 山崎和彦、山崎智子
  • Organizer: BMB2008
  • Place of Presentation: 神戸