Sequence-specificity and binding-cooperativity of multiple DNA-binding domains of CUT-homeodomain transcription factors
| Project/Area Number |
20570119
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
YAMASAKI Kazuhiko National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 主任研究員 (00358243)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA結合タンパク質 / 協同性と競合性 / 転写制御 / 免疫細胞分化 / NMR分光解析 / 等温滴定熱量分析 / 認識コンセンサス配列 / X線結晶解析 / 表面プラズモン共鳴 |
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| Research Abstract |
The DNA-binding mechanism by multiple DNA-binding domains of CUT-homeodomain transcription factors were analyzed by methods of structural biology and physical chemistry. NMR and isothermal titration calorimetry analyses revealed that CUTr1 domain and homeodomain of transcription factor SATB1 bind to DNA containing TAATA sequence in a cooperative manner. A SELEX experiment showed that CUTr1 has the major role in determining the sequence specificity.
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Report
(5 results)
Research Products
(4 results)
