Redox- and CO-dependent Transcription Mechanism by NPAS2
| Project/Area Number |
20570121
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
UCHIDA Takeshi Hokkaido University, 大学院・理学研究院, 助教 (30343742)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 時計蛋白質 / 転写制御 / DNA結合 / ラマン分光法 / NPAS2 / 蛋白質 / シグナル伝達 / 生体分子 / NPAS |
|---|
| Research Abstract |
The purpose of this project is to clarify the mechanism of the redoxand CO-dependent transcription by NPAS2. By using resonance Raman technique, we found that CO can bind to heme located inside NPAS2, which leads to a small conformational change around heme peripheral groups. Such a conformational change around heme is a trigger for the protein whole structural change to modulate the affinity of NPAS2 to the target DNA.
|
Report
(4 results)
Research Products
(81 results)
-
[Journal Article] Unusual Heme Binding in the Bacterial Iron Response Regulator Protein (Irr) : SpectralCharacterization of Heme Binding to HemeRegulatory Motif.2011
Author(s)
Ishikawa, H., Nakagaki, M., Bamba, A., Uchida T., Hori, H., O'Brian, M.R., Iwai, K., Ishimori, K.
-
Journal Title
Biochemistry 50
Pages: 1016-1022
Related Report
Peer Reviewed
-
-
-
[Journal Article] Molecular oxygen regulates theenzymatic activity of a heme-containing diguanylate cyclase (HemDGC) for the synthesis of cyclic di-GMP2010
Author(s)
Sawai, H., Yoshioka, S., Uchida T., Hyodo, M., Hayakawa, Y., Ishimori, K., Aono, S.
-
Journal Title
Biochimica et Biophysica Acta 1804
Pages: 166-172
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
