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Analysis of autoimmune responses involved in the vertebrate organogenesis

Research Project

Project/Area Number 20570125
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionNiigata University

Principal Investigator

IZUTSU Yumi  Niigata University, 自然科学系, 助教 (20301921)

Co-Investigator(Kenkyū-buntansha) ITO Michihiko  北里大学, 理学部, 准教授 (90240994)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords免疫生化学 / アフリカツメガエル / 両生類 / 幼生 / 皮膚 / 細胞死 / T細胞 / 免疫 / 抗原 / 尾の退縮 / 両生類の変態
Research Abstract

During ontogenesis, the vertebrates including Xenopus frogs show morphological changes from embryonic- (larval-) tissues into the adult counterparts. This process is called tissue remodeling. The most drastic tissue remodeling is seen in the tail regression during amphibian metamorphosis. We have preciously proposed a model that the adult-type immune cells, which are newly differentiated during metamorphosis, recognize and remove own tail as non-self. In other words, we postulated that the acquired immune system contributes not only self-defense but also to remodeling processes in animal morphogenesis. This unique model isn't represented except for us. Recently, we isolated genes encoding the antigen proteins, Ouro1 and Ouro2, as candidates being targets for adult immune T cells. In this study, to clarify function of these ouro1 and ouro2 genes, 1), we produced F1, F2 and F3 of ouro-transgenic tadpoles to analyze gain- and loss-of function. 2), Results indicated that expressions of both Ouro1 and Ouro2 proteins are required for the tadpole tail regression. 3), By immunohistochemical analyses, it was shown that Ouro1 and Ouro2 became co-expressed in the tail epidermal cells at metamorphic periods. 4), we identified Ouro ortholog from other amphibian (urodela). It was also expressed during metamorphosis of salamander. These results suggest that this Ouro-mediated system may contribute to more general processes in vertebrate morphogenesis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (43 results)

All 2010 2009 2008 2007 Other

All Journal Article (16 results) (of which Peer Reviewed: 7 results) Presentation (23 results) Remarks (4 results)

  • [Journal Article] Opposite roles of DMRT1 and its W-linked paralogue, DM-W in sexual dimorphism of Xenopus laevis : Implications of a ZZ/ZW-type sex determining system.2010

    • Author(s)
      Yoshimoto S, Ikeda N, Izutsu Y, Shiba T, Takamatsu N, Ito M.
    • Journal Title

      Development 137

      Pages: 2519-2526

    • Related Report
      2010 Final Research Report
  • [Journal Article] Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo.2010

    • Author(s)
      Kurauchi N, Izutsu Y, Maeno M.
    • Journal Title

      Differentiation 79(4-5)

      Pages: 251-259

    • Related Report
      2010 Final Research Report
  • [Journal Article] Identification and expression of ventrally associated leucine-zipper(VAL)in Xenopus embryo.2010

    • Author(s)
      Saito Y, Takahashi Y, Izutsu Y, Maeno M.
    • Journal Title

      Int.J.Dev.Biol. 54(1)

      Pages: 203-208

    • Related Report
      2010 Final Research Report
  • [Journal Article] Opposite roles of DMRT1 and its W-linked paralogue, DM-W in sexual dimorphism of Xenopus laevis : Implications of a ZZ/ZW-typesex determining system.2010

    • Author(s)
      Yoshimoto S., Ikeda N., IzutsuY., Shiba T., Takamatsu N., Ito M.
    • Journal Title

      Development

      Volume: 137 Pages: 2519-2526

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo.2010

    • Author(s)
      Kurauchi N, Izutsu Y, Maeno M.
    • Journal Title

      Differentiation

      Volume: 79 Pages: 251-259

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and expression of ventrally associated leucine-zipper (VAL) in Xenopus embryo.2010

    • Author(s)
      Saito Y, Takahashi Y, Izutsu Y, Maeno M.
    • Journal Title

      Int.J.Dev.Biol. 54

      Pages: 203-208

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis.2009

    • Author(s)
      Mukaigasa K, Hanasaki A, Maeno M, Fujii H, Hayashida S, Itoh M, Kobayashi M, Tochinai S, Hatta M, Iwabuchi K, Taira M, Onoe K, Izutsu Y.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 106

      Pages: 18309-18314

    • Related Report
      2010 Final Research Report
  • [Journal Article] The immune system is involved in Xenopus metamorphosis.2009

    • Author(s)
      Izutsu Y.
    • Journal Title

      Front.Biosci. 14

      Pages: 141-149

    • Related Report
      2010 Final Research Report
  • [Journal Article] Involvement of AP-2rep in morphogenesis of the axial mesoderm in Xenopus embryo.2009

    • Author(s)
      Saito Y, Gotoh M, Ujiie Y, Izutsu Y, Maeno M.
    • Journal Title

      Cell.Tissue.Res. 335(2)

      Pages: 357-369

    • Related Report
      2010 Final Research Report
  • [Journal Article] The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis.2009

    • Author(s)
      Mukaigasa K, Hanasaki A, Maeno M, Fujii H, Hayashida S, Itoh M, Kobayshi M, Tochinai S, Hatta M, Iwabuchi K, Taira M, Onoe K, Izutsu Y.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 106

      Pages: 18309-18314

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] おたまじゃくの尾はなぜ消えるのですか?2009

    • Author(s)
      井筒ゆみ
    • Journal Title

      STAFF News letter 20

      Pages: 6-6

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 免疫学的な観点から見るツメガエルの発生2009

    • Author(s)
      井筒ゆみ
    • Journal Title

      ISI News letter 17

      Pages: 18-18

    • Related Report
      2009 Annual Research Report
  • [Journal Article] The immune system is inovolved in Xenopus metamorphosis2009

    • Author(s)
      Izutsu, Y.
    • Journal Title

      Front. Biosci. 14

      Pages: 141-149

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of AP-2rep in morphogenesis of the axial me soderm in Xenopus embryo.2009

    • Author(s)
      Saito Y, Gotoh M, Ujiie Y, Izutu Y, Maeno M.
    • Journal Title

      Cell Tissue Res. 3352

      Pages: 357-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A role of D domain-related proteins in differentiation and migration of embryonic cells in Xenopus laevis.2008

    • Author(s)
      Shibata T, Takahashi Y, Tasaki J, Saito Y, Izutsu Y, Maeno M.
    • Journal Title

      Mech.Dev. 125(3-4)

      Pages: 284-298

    • Related Report
      2010 Final Research Report
  • [Journal Article] A role of D domain-related proteins in differentiation and migration of embryonic cells in Xenopus laevi s2008

    • Author(s)
      Shibata T, Takahashi Y, Tasaki J, Saito Y, Izutu Y, Maeno. M.
    • Journal Title

      Mech Dev. 125

      Pages: 284-298

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] オタマジャクシの尻尾の消失に関わる免疫系とマクロファージ2010

    • Author(s)
      井筒ゆみ
    • Organizer
      第50回日本リンパ網内系学会総会シンポジウム講演
    • Place of Presentation
      新潟朱鷺メッセ(招待講演)
    • Related Report
      2010 Final Research Report
  • [Presentation] 「A possible reason why both ouro1 and ouro2 genes are required for tail regression during tadpole metamorphosis」(おたまじゃくしの変態時の尾部退縮になぜオウロ1とオウロ2両方の遺伝子が必要なのか)2010

    • Author(s)
      Tomomi Oshima, Tomohiro Kikuta, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      第43回日本発生生物学会
    • Place of Presentation
      京都国際会館,京都
    • Related Report
      2010 Final Research Report
  • [Presentation] A role of the immune system in the tadpole tail degeneration during Xenopus metamorphosis2010

    • Author(s)
      Yumi Izutsu, Tomomi Oshima, Shin-ichiro Hayashida, Akira Hanasaki, Mitsugu Maeno
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      Lake Louise, Alberta, Canada.(招待講演)
    • Related Report
      2010 Final Research Report
  • [Presentation] Knockdown of ouro genes inhibit tadpole tail regression during Xenopus metamorphosis2010

    • Author(s)
      Akira Hanasaki, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      Lake Louise, Alberta, Canada.
    • Related Report
      2010 Final Research Report
  • [Presentation] A possible reason why both ouro1 and ouro2 genes are required for tail regression during Xenopus metamorphosis2010

    • Author(s)
      Tomomi Oshima, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      Lake Louise, Alberta, Canada.
    • Related Report
      2010 Final Research Report
  • [Presentation] 「ツメガエル細胞株A6における尾部抗原タンパクOuroの細胞内局在の変化」(Changes of intracellular localizations of the tail antigen protein Ouro in cultured Xenopus A6 cells.)2010

    • Author(s)
      菊田智広, 大嶋友美, 前野貢,井筒ゆみ
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京大学教養棟,東京
    • Related Report
      2010 Final Research Report
  • [Presentation] The role of the immune system in the tail tissue degeneration during amphibian metamorphosis(両生類の変態過程における尾の退縮に関わる免疫システム)2010

    • Author(s)
      井筒ゆみ
    • Organizer
      第33回日本分子生物学会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(招待講演)
    • Related Report
      2010 Final Research Report
  • [Presentation] オタマジャクシの尻尾の消失に関わる免疫系とマクロファージ)2010

    • Author(s)
      井筒ゆみ
    • Organizer
      第50回日本リンパ網内系学会総会
    • Place of Presentation
      新潟(朱鷺メッセ)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Apossible reason why both ourol and ouro2 genes are required for tail regression during tadpole metamorphosis.2010

    • Author(s)
      Tomomi Oshima, Tomohiro Kikuta, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      第43回日本発生生物学会
    • Place of Presentation
      京都(京都国際会館)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Arole of the immune system in the tadpole tail degeneration during Xeuopus metamorphosis2010

    • Author(s)
      Yumi Izutsu, Tomomi Oshima, Shin-ichiro Hayashida, Akira Hanasaki, Mitsugu Maeno
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      カナダ(Lake Louise)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Knockdown of ouro.genes inhibit tadpole tail regression during Xeuopus metamorphosis.2010

    • Author(s)
      Akira Hanasaki, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      カナダ(Lake Louise)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Apossible reason why both ourol and ouro2 genes are required for tail regression during Xenopus metamorphosis.2010

    • Author(s)
      Tomomi Oshima, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      13th International Xenopus Conference
    • Place of Presentation
      カナダ(Lake Louise)
    • Related Report
      2010 Annual Research Report
  • [Presentation] ツメガエル細胞株A6における尾部抗原タンパクOuroの細胞内局在の変化2010

    • Author(s)
      菊田智広, 大嶋友美, 前野貢,井筒ゆみ
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京(東京大学教養棟)
    • Related Report
      2010 Annual Research Report
  • [Presentation] The role of the immune system in the tail tissue degeneration during amphibian metamorphosis.2010

    • Author(s)
      井筒ゆみ
    • Organizer
      第33回日本分子生物学会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸(神戸ポートアイランド)(招待講演)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 「Knockdown of ouro genes in tail regression in transgenic Xenopus tadpoles」(ノックダウン法によるツメガエル幼生尾部抗原遺伝子オウロボロスの機能解析)2009

    • Author(s)
      Akira Hanasaki, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟(ベストポスター賞受賞)
    • Related Report
      2010 Final Research Report
  • [Presentation] 「Role of ouro1 and ouro2 genes in tail degeneration during Xenopus metamorphosis」(ツメガエル幼生尾部退縮に働く抗原遺伝子オウロボロス)2009

    • Author(s)
      Shin-ichiro Hayashida, Mitsugu Maeno, Yumi Izutsu
    • Organizer
      日本発生生物学会大42回大会
    • Place of Presentation
      新潟
    • Related Report
      2010 Final Research Report
  • [Presentation] 「ツメガエル尾部退縮における免疫系の関与」(Immune system involved in Xenopus tail regression during metamorphosis)2009

    • Author(s)
      五十嵐一博、前野貢、井筒ゆみ
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Related Report
      2010 Final Research Report
  • [Presentation] Knockdown of ouro genes in tail regression in transgenic Xenopus tadpoles. (ベストポスター賞受賞)2009

    • Author(s)
      ^○Akira Hanasaki, Mitsugu Maeno, Yumi lzutsu
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟(朱鷺メッセ)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of ouro1 and ouro2 genes in tail degeneration during Xenopus metamorphosis.2009

    • Author(s)
      ^○Shin-ichiro Hayashida, Mitsugu Maeno, Yumi lzutsu
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟(朱鷺メッセ)
    • Related Report
      2009 Annual Research Report
  • [Presentation] ツメガオル尾部退縮における免疫系の関与.2009

    • Author(s)
      五十嵐一博^1、前野貢^2、井筒ゆみ^<2○>
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡(静岡大学)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Requirement of keratin-related protein Ouroboros as immune antigens for metamorphic tail regression in Xenopus2008

    • Author(s)
      Yumi Izutsu, Mitsugu Maeno, Masanori Taira, Kazunori Onoee
    • Organizer
      12th International Xenopus Conference
    • Place of Presentation
      Leiwen, Germany.
    • Related Report
      2010 Final Research Report
  • [Presentation] Reouirement of kerat in-related protein Ouroboros as immune antigens for metamorphic tail regression in Xenopus2008

    • Author(s)
      Yumi Izutsu, Mitsugu Maeno, Masanori Taira, Kazunori Onoe
    • Organizer
      International Xenopus Conference
    • Place of Presentation
      Leiwen, Germany
    • Related Report
      2008 Annual Research Report
  • [Presentation] Role of ouro genes in tail degeneration during Xenopus metamorphosis2007

    • Author(s)
      Katsuki Mukaigasa, Mitsugu Maeno, Masanori Taira, Kazunori Onoe, Yumi Izutsu
    • Organizer
      日本発生生物学会第40回大会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等 研究室ホームページ(申請者らが作製)

    • URL

      http://www.sc.niigata-u.ac.jp/biologyindex/izutsu/welcome.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 新潟大学ホームページ(研究トピックスとし研究内容が掲載されている)

    • URL

      http://www.niigata-u.ac.jp/research/10_research_010/ouroboros.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 新潟大学ホームページ「研究トピックス」として

    • URL

      http://www.niigata-u.ac.jp/research/10_research_010.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.sc.niigata-u.ac.jp/biologyindex/izutsu/welcome.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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