Changes in activity of the transcriptional regulator Id by toxic trace elements and redox signaling.

• Project/Area Number: 20570128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: University of Fukui
• Principal Investigator: KUROOKA Hisanori University of Fukui, 医学部, 准教授 (00293879)
• Co-Investigator(Renkei-kenkyūsha): SUGAI Manabu 京都大学, 医学(系)研究科(研究院), 講師 (90303891)
• Research Collaborator: MATSUI Yukie 福井大学, 医学部, 技術補佐員
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Id / 細胞内局在 / ヒ素 / ジスルフィド結合 / レドックスシグナル / システイン / 酸化ストレス / ホモ多量体 / HLHタンパク質 / 過酸化水素水 / Eタンパク質 / ヘテロ二量体 / HLHタンバク質 / NES活性 / Id3 / 有毒微量元素 / 初期応答遺伝子 / Ets型転写因子 / 転写抑制

Research Abstract

To understand the regulation of interaction between the transcriptional repressor Id and other transcription factors, we analyzed the subcellular localization of Id3 regulated by toxic trace elements such as arsenite and cadmium, and the intramolecular disulfide bonds formed in the HLH proteins by redox signaling. Although the involvement of cysteine residues has been shown in both cases, they differ in which residues are important, suggesting that there are various regulations for the interaction.

Research Products

• [Journal Article] Identification of BMP-responsive elements in the mouse Id2 gene. (2010)
  • Author(s): Nakahiro, T., Kurooka, H., Mori, K., Sano, K., Yokota, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 第399巻 Pages: 416-421
  • Peer Reviewed
• [Journal Article] Glutathione S-transferase M1 inhibits dexamethasone-induced apoptosis in association with the suppression of Bim through dual mechanisms in a lymphoblastic leukemia cell line. (2010)
  • Author(s): Hosono, N., Kishi, S., Iho, S., Urasaki, Y., Yoshida, A., Kurooka, H., Yokota, Y., Ueda, T.
  • Journal Title: Cancer Sci. 第101巻 Pages: 767-773
  • NAID: 10026589943
  • Peer Reviewed
• [Journal Article] Identification of BMP responsive elements in the mouse Id2 gene. (2010)
  • Author(s): Nakahiro T, et al.
  • Journal Title: Biochem.Biophys.Res.Commun Volume: 399 Pages: 416-421
  • Peer Reviewed
• [Journal Article] Glutathione S-transferase M1 inhibits dexamethasone-induced apoptosis in association with the suppression of Bim through dual mechanisms in a lymphoblastic leukemia cell line (2010)
  • Author(s): Hosono N., et al.
  • Journal Title: Cancer Science 101 Pages: 767-773
  • Peer Reviewed
• [Presentation] 酸化ストレスにより形成されるHLHタンパク質の分子間ジスルフィド結合 (2010)
  • Author(s): 黒岡尚徳、横田義史
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] 酸化ストレスにより形成されるHLHタンパク質の分子間ジスルフィド結合 (2010)
  • Author(s): 黒岡尚徳
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] BMPシグナルによる転写調節因子Id2の遺伝子発現制御機構 (2009)
  • Author(s): 中廣剛士、黒岡尚徳、佐野和生、横田義史
  • Organizer: 日本生化学会北陸支部第27回大会
  • Place of Presentation: 福井
  • Year and Date: 2009-05-23
• [Presentation] BMPシグナルによる転写調節因子Id2の遺伝子発現機構の解析 (2008)
  • Author(s): 中廣剛士、黒岡尚徳、佐野和生、横田義史
  • Organizer: 第31回日本分子生物学会年会/第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] BMPシグナルによる転写調節因子Id2の遺伝子発現機構の解析 (2008)
  • Author(s): 中廣剛士
  • Organizer: 第31回日本分子生物学会年会/第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Remarks] ホームページ等
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