| Project/Area Number |
20570128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Fukui |
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Principal Investigator |
KUROOKA Hisanori University of Fukui, 医学部, 准教授 (00293879)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGAI Manabu 京都大学, 医学(系)研究科(研究院), 講師 (90303891)
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| Research Collaborator |
MATSUI Yukie 福井大学, 医学部, 技術補佐員
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Id / 細胞内局在 / ヒ素 / ジスルフィド結合 / レドックスシグナル / システイン / 酸化ストレス / ホモ多量体 / HLHタンパク質 / 過酸化水素水 / Eタンパク質 / ヘテロ二量体 / HLHタンバク質 / NES活性 / Id3 / 有毒微量元素 / 初期応答遺伝子 / Ets型転写因子 / 転写抑制 |
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| Research Abstract |
To understand the regulation of interaction between the transcriptional repressor Id and other transcription factors, we analyzed the subcellular localization of Id3 regulated by toxic trace elements such as arsenite and cadmium, and the intramolecular disulfide bonds formed in the HLH proteins by redox signaling. Although the involvement of cysteine residues has been shown in both cases, they differ in which residues are important, suggesting that there are various regulations for the interaction.
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