Analysis of the interactions of collectins with immunoglobuins and the regulation immunoglobulin functions

• Project/Area Number: 20570136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Sapporo Medical University
• Principal Investigator: SHIMIZU Takeyuki Sapporo Medical University, 教育研究部・医療学系, 准教授 (10339137)
• Co-Investigator(Kenkyū-buntansha): KUROKI Yoshio 札幌医科大学, 医学部, 教授 (70161784) ; NISHITANI Chiaki 札幌医科大学, 医学部, 助教 (30381255) ; ARIKI Shigeru 札幌医科大学, 医学部, 助教 (80464478)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 免疫学 / 蛋白質 / 生体分子 / コレクチン / 免疫グロブリン

Research Abstract

We found that MBL and SP-D bound to IgM through CRD. The interactions between MBL and IgM in immune complexes depended on the affinity and antigen-density. These results suggest that antigen-bound IgM can take different conformations, which are distinguished by MBL. We also found that MBL can modulate classical pathway of complement cascade.

Research Products

• [Journal Article] The amino acid residue at position 95 and the third CDR region in the H chain determine the ceiling affinity and the maturation pathway of an anti-(4-hydroxy-3-nitrophenyl) acetyl antibody (2010)
  • Author(s): Akikazu Murakami, Yuka Takahashi, Miyuki Nishimura, Takeyuki Shimizu, Takachika Azuma.
  • Journal Title: Mol.Immunol. vol.48 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Pulmonary collectins protect macrophages against pore-forming activity of Legionella pneumophlla and suppress its intracellular growth (2010)
  • Author(s): Kaku Sawada, Shigeru Ariki, Takashi Kojima, Atsushi Saito, Masami Yamazoe, Chiaki Nishitani, Takeyuki Shimizu, Motoko Takahashi, Hiroaki Mitsuzawa, Shin-ichi Yokota, Norimasa Sawada, Nobuhiro Fujii, Hiroki Takahashi, Yoshio Kuroki.
  • Journal Title: J.Biol.Chem. vol.285 Pages: 8434-8443
  • Peer Reviewed
• [Journal Article] The amino acid residue at position 95 and the third CDR region in the H chain determine the ceiling affinity and the maturation pathway of an anti-(4-hydroxy-3-nitrophenyl)acetyl antibody (2010)
  • Author(s): Murakami A.
  • Journal Title: Mol.Immunol. Volume: Vol.48 Pages: 48-58
  • Peer Reviewed
• [Journal Article] Pulmonary collectins protect macrophages against pore-forming activity of Legionella pneumophila and suppress its intracellular growth (2010)
  • Author(s): Sawada K.
  • Journal Title: J.Biol.Chem. 285 Pages: 8434-8443
  • Peer Reviewed
• [Journal Article] Mannose binding lectin and lung collectins interact with Toll-like receptor 4 and MD-2 by different mechanisms (2009)
  • Author(s): Takeyuki Shimizu, Chiaki Nishitani, Hiroaki Mitsuzawa, Shigeru Ariki, Motoko Takahashi, Katsuki Ohtani, Nobutaka Wakamiya, Yoshio Kuroki.
  • Journal Title: Biochim.Biophys.Acta. vol.1790 Pages: 1705-1710
  • Peer Reviewed
• [Journal Article] Mutational analysis of Cys^<88> of Toll-like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression (2009)
  • Author(s): Chiaki Nishitani, Motoko Takahashi, Hiroaki Mitsuzawa, Takeyuki Shimizu, Shigeru Ariki, Norio Matsushima, Yoshio Kuroki.
  • Journal Title: Int.Immunol. vol.21 Pages: 925-934
  • NAID: 10025387642
  • Peer Reviewed
• [Journal Article] Mutational analysis of Cys^<88> of Toll-like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression (2009)
  • Author(s): Nishitani C.
  • Journal Title: Int.Immunol. 21 Pages: 925-934
  • NAID: 10025387642
  • Peer Reviewed
• [Journal Article] Mannose binding lectin and lung collectins interact with Toll-like receptor 4 and MD-2 by different mechanisms (2009)
  • Author(s): Shimizu T.
  • Journal Title: Biochim.Biophys.Acta. 1790 Pages: 1705-1710
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D inhibits lipopolysaccharide (LPS)-induced inflammatory cell responses by altering LPS binding to its receptors (2008)
  • Author(s): Masami Yamazoe, Chiaki Nishitani, Motoko Takahashi, Tsuyoshi Katoh, Shigeru Ariki, Takeyuki Shimizu, Hiroaki Mitsuzawa, Kaku Sawada, Dennis R.Voelker, Hiroki Takahashi, Yoshio Kuroki.
  • Journal Title: J.Biol.Chem. vol.283 Pages: 35878-35888
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D binds MD-2 through the carbohydrate recognition domain (2008)
  • Author(s): Xiaomeng Nie, Chiaki Nishitani, Masami Yamazoe, Shigeru Ariki, Motoko Takahashi, Takeyuki Shimizu, Hiroaki Mitsuzawa, Kaku Sawada, Kelly Smith, Erika Crouch, Hisato Nagae, Hiroki Takahashi, Yoshio Kuroki.
  • Journal Title: Biochemistry vol.47 Pages: 12878-12885
• [Journal Article] Pulmonary surfactant protein D inhibits lipopolysaccharide (LPS)induced inflammatory cell responses by altering LPS binding to its receptors. (2008)
  • Author(s): Yamazoe M.
  • Journal Title: J. Biol. Chem. 283 Pages: 35878-35888
  • Peer Reviewed
• [Journal Article] Pulmonary surfactant protein D binds MD-2 through the carbohydrate recognition domain. (2008)
  • Author(s): Nie, X
  • Journal Title: Biochemistry 47 Pages: 12878-12885
  • Peer Reviewed
• [Presentation] Pulmonary collectins bind and agglutinate M.avium and attenuate its growth (2010)
  • Author(s): 有木茂
  • Organizer: 第83回日本生化学会、第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] コレクチンの免疫グロブリンに対する結合性の解析 (2010)
  • Author(s): 清水健之
  • Organizer: 第83回日本生化学会、第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] コレクチンの免疫グロブリンに対する結合性の解析 (2010)
  • Author(s): 清水健之
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Pulmonary collectins bind and agglutinate M.avium and attenuate its growth (2010)
  • Author(s): 有木茂
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 酸化肺サーファクタントリン脂質によるLPS惹起炎症性細胞応答制御機構の解明 (2010)
  • Author(s): 西谷千明
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 非定型抗酸菌に対する肺コレクチンの生体防御機構 (2010)
  • Author(s): 有木茂
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 酸化肺サーファクタントリン脂質によるLPS惹起炎症性細胞応答制御機構の解明 (2010)
  • Author(s): 西谷千明
  • Organizer: 第83回日本生化学会、第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 肺サーファクタントリン脂質の酸化によるLPS惹起炎症性細胞応答の抑制 (2009)
  • Author(s): 西谷千明
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2009-10-25
• [Presentation] 肺サーファクタントリン脂質の酸化によるLPS惹起炎症性細胞応答の抑制 (2009)
  • Author(s): 西谷千明
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] 非定型抗酸菌に対する肺コレクチンの生体防御機構 (2009)
  • Author(s): 有木茂
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] Pulmonary collectins exhibit anti-microbial activity against Legionella pneumophila (2008)
  • Author(s): Sawada K.
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Pulmonary collectins interact with and aggregate Mycobacteriumavium (2008)
  • Author(s): Ariki S.
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] Pulmonary collectins interact with and aggregate Mycobacterium avium (2008)
  • Author(s): 有木茂
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2008-12-11
• [Presentation] Pulmonary collectins exhibit anti-microbial activity against Legionella pneumophila (2008)
  • Author(s): 澤田格
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2008-12-11
• [Presentation] Mutational analysis of Cys^<88> of Toll-Like receptor 4 highlights the critical role of MD-2 in cell surface receptor expression (2008)
  • Author(s): Nishitani C.
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-11
• [Presentation] 肺サーファクタント蛋白質Dによるリボ多糖惹起炎症反応の抑制 (2008)
  • Author(s): 山添雅己
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 北海道大学学術交流会館(北海道)
  • Year and Date: 2008-07-10
• [Presentation] 肺コレクチンは非定型抗酸菌に結合し、菌体凝集を惹起する (2008)
  • Author(s): 有木茂
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 第19回日本生体防御学会学術総会
  • Year and Date: 2008-07-10
• [Presentation] 肺コレクチンは非定型抗酸菌に結合し菌体凝集を惹起する (2008)
  • Author(s): 有木茂
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-10
• [Presentation] レジオネラ菌に対する肺コレクチンの増殖抑制作用 (2008)
  • Author(s): 深田格
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-10
• [Presentation] TLR4の細胞表面発現におけるMD-2の重要性 (2008)
  • Author(s): 西谷千明
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-10
• [Presentation] 肺サーフアクタント蛋白質Dによるリポ多糖惹起炎症反応の抑制 (2008)
  • Author(s): 山添雅己
  • Organizer: 第19回日本生体防御学会学術総会
  • Place of Presentation: 北海道大学学術交流会館
  • Year and Date: 2008-07-10