Studies on orphan G protein-coupled receptors which are related to body size : ligand search and their functions

• Project/Area Number: 20570140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Tohoku Pharmaceutical University
• Principal Investigator: MITOMA Junya Tohoku Pharmaceutical University, 薬学部, 講師 (10281627)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: シグナル伝達 / 糖 / 糖尿病 / GPCR / リガンド / Gタンパク共役型受容体 / オーファン受容体 / cAMP / カルシウムシグナリング / ノックアウトマウス / インスリン

Research Abstract

We found that the mice deficient in G protein-coupled receptor Rigp2 (Rigp2-/-) are significantly smaller than wild-type mice. Because the number of Rigp2-/- mice born from heterozygote mating is much smaller than that of Rigp2+/- and +/+ mice, there may be some defect during development. We also found that the only one of Asp residues of this protein has N-linked glycans. We could not identify a ligand for this GPCR.

Research Products

• [Journal Article] Prominent expression of sialyl Lewis X-capped core 2-branched O-glycans on high endothelial venule-like vessels in gastric MALT lymphoma. (2011)
  • Author(s): Kobayashi M, Mitoma J, Hoshino H, Yu S-Y, Shimojo Y, Suzawa K, Khoo K-H, Fukuda M, Nakayama J.
  • Journal Title: J.Pathol. 224 Pages: 67-77
• [Journal Article] Core O-glycans required for lymphocyte homing : gene knockout mice of core 1 beta1,3-N-acetylglucosaminyltransferase and core 2 N-acetylglucosaminyltransferase. (2010)
  • Author(s): Mitoma, J., Fukuda, M.
  • Journal Title: Methods Enzymol. 479 Pages: 257-257
• [Journal Article] Core O-glycans required for lymphocyte homing gene knockout mice of core 1 beta 1,3-N-acetylglucosaminyltransferase and core 2 N-acetylglucosaminyltransferase. (2010)
  • Author(s): Junya Mitoma, Minoru Fukuda
  • Journal Title: Methods Enzymol. Volume: 479 Pages: 257-270
• [Journal Article] The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells. (2009)
  • Author(s): Mitoma, J., Miyazaki, T., Sutton-Smith, M., Suzuki, M., Saito, H., Yeh, J. C., Kawano, T., Hindsgaul, O., Seeberger, P.H., Panico, M., Haslam, S.M., Morris, H.R., Cummings, R.D., Dell, A., Fukuda, M.
  • Journal Title: Glycoconj.J. 26(5) Pages: 511-511
• [Journal Article] The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells (2009)
  • Author(s): Mitoma J, Miyazaki T, Sutton-Smith M, Suzuki M, Saito H, Yeh JC, Kawano T, Hindsgaul O, Seeberger PH, Panico M, Haslam SM, Morris HR, Cummings RD, Dell A, Fukuda Ma
  • Journal Title: Glycoconjugate Journal 26 Pages: 511-523
  • Peer Reviewed
• [Journal Article] Oligosaccharides in lymphocyte homing.[リンパ球ホーミング における修飾糖鎖] (2008)
  • Author(s): Mitoma, J.
  • Journal Title: Tanpakushitu Kakusan Koso 53(12) Pages: 1598-1598
• [Presentation] キメラタンパク質の作製によるマウスシアリダーゼNeu3およびNeu2の性質解析 (2009)
  • Author(s): 中川哲人、三苫純也、渡辺俊、宮城妙子、東秀好
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-24
• [Remarks] ホームページ等
  • URL: http://www.tohoku-pharm.ac.jp/laboratory/seitaijo/JAPANESE/PUBLICATIONS-J.html
• [Remarks]
  • URL: http://www.tohoku-pharm.ac.jp/laboratory/seitaijo/JAPANESE/TOP-J.html