Research for structure and function of bacterial cation transport systems

• Project/Area Number: 20570141
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: NAKAMURA Tatsunosuke 新潟薬科大学, 薬学部, 教授 (20114308)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: カチオン輸送 / 交換輸送系 / 膜タンパク質 / 細胞内イオン調節 / 細胞内pH調節 / Na^+/H^+交換輸送系 / NHE / NhaA / K^+/H^+交換輸送 / NhaP2 / 構造と機能 / アンチポーター / 物質輸送 / イオン輸送機構 / 細胞生物学 / エネルギー代謝 / イオン輸送

Research Abstract

What is the function of many membrane proteins written in the genome of Vibrio? Which is the gene that code K+/H+antiporter function for internal pH regulation in Vibrio? Does internal Ca^<2+> concentration be maintained low? If so, what kind of transporter does the task. What kind of real functions of Pha system, NhaP2, Fku, Trk and Ktr in Vibrio cells? We looked forward such queries and found some answers.

Research Products

• [Journal Article] Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production (2011)
  • Author(s): Ostash B, Rebets Y, Myronovskyy M, Tsypik O, Ostash I, Kulachkovskyy O, Datsuk Y, Nakamura T, Walker S, Fedorenko V
  • Journal Title: Microbiology Volume: 157 Pages: 1240-1249
• [Journal Article] Identification and characterization of the Streptomyces globisporus 1912 regulatory gene 1ndYR that affects sporulation and antibiotic production (2011)
  • Author(s): Ostash B, 他
  • Journal Title: Microbiology Volume: 157 Issue: 4 Pages: 1240-1249
  • DOI: 10.1099/mic.0.045088-0
  • Peer Reviewed
• [Journal Article] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117 (2010)
  • Author(s): Horbal L, Rebets Y, Rabyk M, Luzhetskyy A, Ostash B, Welle E, Nakamura T, Fedorenko V, Bechthold A
  • Journal Title: Appl Microbiol Biotechnol Volume: 85 Pages: 1069-1079
• [Journal Article] Glultamate 85 is involved in the sodium/proton exchange activity of the Escherichia coli ChaA (2010)
  • Author(s): Fukaya F, Tanaka K, Waditee R, Tanaka Y, Nakamura T, Takabe T
  • Journal Title: Biosci Biotechnol Biochem Volume: 74 Pages: 1116-1119
  • NAID: 10027555683
• [Journal Article] Glultamate 85 is involved in the sodium/proton exchange activity of the Escherichia coli ChaA. (2010)
  • Author(s): Fukaya F, Tanaka K, Waditee R, Tanaka Y, Nakamura T, Takabe T.
  • Journal Title: Biosci Biotechnol Biochem. 74 Pages: 1116-1119
  • NAID: 10027555683
  • Peer Reviewed
• [Journal Article] Glutamate 85 is involved in the sodium/proton exchange activity of the Escherichia coli Cha A. (2010)
  • Author(s): Fukaya F
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 1116-1119
  • Peer Reviewed
• [Journal Article] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. (2010)
  • Author(s): Horbal L, 他
  • Journal Title: Appl Microbiol Biotechnol Volume: 85 Pages: 1069-1079
  • Peer Reviewed
• [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation. (2009)
  • Author(s): Ito T, Uozumi N, Nakamura T, Takayama S, Matsuda N, Aiba H, Hemmi H, Yoshimura T
  • Journal Title: Biosci Biotechnol Biochem Volume: 73 Pages: 2698-2704
  • NAID: 10027549163
• [Journal Article] An Mrp-like cluster in the halotolerant cyanobacterium Aphanothece halophytica functions as a Na^+/H^+antiporter (2009)
  • Author(s): Fukaya F, Promden W, Hibino T, Tanaka Y, Nakamura T, Takabe T
  • Journal Title: Appl Environ Microbiol Volume: 75 Pages: 6626-6629
  • NAID: 130006995859
• [Journal Article] pH-dependent regulation of the multi-subunit cation/proton antiporter Pha1 system from Sinorhizobium meliloti (2009)
  • Author(s): Yamaguchi T, Tsutsumi F, Putnoky P, Fukuhara M, Nakamura T
  • Journal Title: Microbiology Volume: 155 Pages: 2750-2756
• [Journal Article] Identification and characterization of the Na+/H+antiporter NhaS3 from the thylakoid membrane of Synechocystis sp. PCC 6803 (2009)
  • Author(s): Tsunekawab K, Shijukua T, Hayashimotoa M, Kojimac Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuokai K, Omata T, Uozumia N
  • Journal Title: Journal of Biological Chemistry Volume: 284 Pages: 16513-16521
• [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation. (2009)
  • Author(s): Ito T, Uozumi N, Nakamura T, Takayama S, Matsuda N, Aiba H, Hemmi H, Yoshimura T.
  • Journal Title: Biosci Biotechnol Biochem. 73 Pages: 2698-2704
  • NAID: 10027549163
  • Peer Reviewed
• [Journal Article] An Mrp-like cluster in the halotolerant cyanobacterium Aphanothece halophytica functions as a Na^+/H^+ antiporter. (2009)
  • Author(s): Fukaya F, Promden W, Hibino T, Tanaka Y, Nakamura T, Takabe T.
  • Journal Title: Appl Environ Microbiol. 75 Pages: 6626-6629
  • NAID: 130006995859
• [Journal Article] pH-dependent regulation of the multi-subunit cation/proton antiporter Pha1 system from Sinorhizobium meliloti. (2009)
  • Author(s): Yamaguchi T, Tsutsumi F, Putnoky P, Fukuhara M, Nakamura T.
  • Journal Title: Microbiology 155 Pages: 2750-2756
• [Journal Article] Identification and characterization of the Na^+/H^+ antiporter NhaS3 from the thylakoid membrane of Synechocystis sp.PCC 6803. (2009)
  • Author(s): Tsunekawab K, 他
  • Journal Title: J.Biol.Chem. 284 Pages: 16513-16521
• [Journal Article] pH-dependent regulation of the multi-subunit cation/proton antiporter Phal system from Sinorhizobium meliloti (2009)
  • Author(s): Yamaguchi T, 他
  • Journal Title: Microbiology 155 Pages: 2750-2756
  • Peer Reviewed
• [Journal Article] The implication of YggT of Escherichia coli in osmotic regulation (2009)
  • Author(s): Ito T, 他
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 2698-2704
  • Peer Reviewed
• [Journal Article] An Mrp-like cluster in the halotolerant cyanobacterium Aphanothece halophytica functions as a Na^+/H^+ antiporter (2009)
  • Author(s): Fukaya F, 他
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 6626-6629
  • Peer Reviewed
• [Journal Article] An ABC transporter encoding gene lndW confers resistance to landomycin E (2008)
  • Author(s): Ostash I, Rebets Y, Ostash B, Kobylyanskyy A, Myronovskyy M, Nakamura T, Walker S, Fedorenko V
  • Journal Title: Arch Microbiol Volume: 190 Pages: 105-109
• [Journal Article] Function of lanI in regulation of landomycin A biosynthesis in Streptomyces cyanogenus S136 and cross-complementation studies with Streptomyces antibiotic regulatory proteins encoding genes (2008)
  • Author(s): Rebets Y, Dutko L, Ostash B, Luzhetskyy A, Kulachkovskyy O, Yamaguchi T, Nakamura T, Bechthold A, Fedorenko V
  • Journal Title: Arch Microbiol Volume: 189 Pages: 111-120
• [Remarks]
  • URL: http://202.244.210.151/nuphp/KgApp