Genome-wide screen and functional analysis of novel factors required for selective degradation of mitochondria

• Project/Area Number: 20570144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Institute for Basic Biology
• Principal Investigator: OKAMOTO Koji National Institute for Basic Biology, 大学院・生命機能研究科, 特任准教授 (40455217)
• Research Collaborator: OKAMOTO Noriko 大阪大学, 大学院・生命機能研究科, 特任研究員 (90568750)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2010: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: ミトコンドリア / オートファジー / 膜輸送 / オルガネラ / ダイナミクス / 品質管理 / 出芽酵母 / 細胞内分解 / 酸化ストレス / タンパク質標的化

Research Abstract

Mitochondria serve as "the power plants of the cell", playing essential roles in cellular functions. Recently, it has been proposed that degradation of mitochondria as whole organelles, termed mitophagy, is critical for mitochondrial quality control. However, the molecular mechanisms remain largely unknown. In this study, we utilized budding yeast, and succeeded to establish selective degradation of mitochondria as a phenomenon to be analyzed, and systematically identify mitophagy-related factors.

Research Products

• [Journal Article] Mitochondria autophagy in yeast. (2011)
  • Author(s): Kanki, T., Klionsky, D.J., Okamoto K.
  • Journal Title: Antioxid.Redox Signal. 14 Pages: 1989-2001
  • Peer Reviewed
• [Journal Article] Structure-function analysis of the yeast mitochondrial Rho GTPase, Gemlp (2011)
  • Author(s): Koshiba, T., et al.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 354-362
  • Peer Reviewed
• [Journal Article] 酵母のマイトファジー,～観察からの始まり～ (2010)
  • Author(s): 岡本徳子, 岡本浩二, 大隅良典
  • Journal Title: 顕微鏡・特集「オートファジー:形態学と分子生物学の融合」 45 Pages: 83-86
  • NAID: 130007788949
• [Journal Article] マイトファジー (2010)
  • Author(s): 岡本浩二, 岡本徳子
  • Journal Title: 細胞工学・特集「その時ミトコンドリアは動いた:独自の品質管理システムから疾患に迫る」 29 Pages: 423-428
• [Journal Article] 選択的ミトコンドリア分解のしくみ (2010)
  • Author(s): 岡本浩二
  • Journal Title: 医学の歩み・第1土曜特集「ここまでわかったミトコンドリア研究の新展開」 232 Pages: 665-670
• [Journal Article] マイトファジー (2010)
  • Author(s): 岡本浩二, 岡本徳子
  • Journal Title: 細胞工学 Volume: 29 Pages: 423-428
• [Journal Article] 酵母のマイトファジー (2010)
  • Author(s): 岡本徳子, 岡本浩二, 大隅良典
  • Journal Title: 顕微鏡 Volume: 45 Pages: 83-86
• [Journal Article] 選択的ミトコンドリア分解のしくみ (2010)
  • Author(s): 岡本浩二
  • Journal Title: 医学のあゆみ 232 Pages: 665-670
• [Journal Article] A landmark protein essential for mitophagy. (2009)
  • Author(s): 岡本浩二, 岡本徳子, 大隅良典
  • Journal Title: Autophagy 5 Pages: 1203-1205
  • Peer Reviewed
• [Journal Article] Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy. (2009)
  • Author(s): Okamoto K., Kondo-Okamoto N., Ohsumi Y.
  • Journal Title: Dev.Cell 17 Pages: 87-97
  • Peer Reviewed
• [Journal Article] A landmark protein essential for mitophagy (2009)
  • Author(s): Koji Okamoto, Noriko-Kondo Okamoto, Yoshinori Ohsumi
  • Journal Title: Autophagy 5 Pages: 1203-1205
• [Journal Article] Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy (2009)
  • Author(s): Koji Okamoto, Noriko-Kondo Okamoto, Yoshinori Ohsumi
  • Journal Title: Developmental Cell 17 Pages: 87-97
  • Peer Reviewed
• [Presentation] Mechanism of Atg32-mediated mitophagy in yeast (2011)
  • Author(s): Koji Okamoto
  • Organizer: Barcelona BioMed Conference Mitochondrial Autophagy
  • Place of Presentation: Institutd d'Estudis Catalans Barcelona(Spain)(招待講演)
  • Year and Date: 2011-03-23
• [Presentation] Mechanism of Atg32-mediated mitophagy in yeast. (2011)
  • Author(s): 岡本浩二
  • Organizer: Barcelona Biomed Conference on Mitochondrial Autophagy.(招待講演(英語))
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 2011-03-22
• [Presentation] 選択的ミトコンドリア分解:現象から分子理解へ (2011)
  • Author(s): 岡本浩二
  • Organizer: 第7回宮崎サイエンスキャンプ(招待講演(日本語)
  • Place of Presentation: 宮崎
  • Year and Date: 2011-02-26
• [Presentation] ミトコンドリア質・量管理システムの分子機構 (2011)
  • Author(s): 岡本浩二
  • Organizer: 第7回宮崎サイエンスキャンプ
  • Place of Presentation: ワールドコンベンションセンター・サミット・宮崎(招待講演)
  • Year and Date: 2011-02-26
• [Presentation] Regulation of mitochondria-specific degradation in yeast. (2010)
  • Author(s): 岡本浩二
  • Organizer: 7th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM) and 10th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit).(招待講演(英語))
  • Place of Presentation: Fukuoka, Japan.
  • Year and Date: 2010-12-17
• [Presentation] Regulation of mitochondria-specific degradation in yeast (2010)
  • Author(s): Koji Okamoto
  • Organizer: 7th Conference of Asian Society for Mitochondrial Research and Medicine and 10th Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: 福岡国際会議場(招待講演)
  • Year and Date: 2010-12-17
• [Presentation] ミトコンドリア品質管理の制御機構 (2010)
  • Author(s): 岡本浩二
  • Organizer: 第69回日本癌学会学術総会,シンポジウム「がんとオートファジー」(招待講演(日本語))
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] ミトコンドリア品質管理の制御機構 (2010)
  • Author(s): 岡本浩二
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(招待講演)
  • Year and Date: 2010-09-23
• [Presentation] Molecular mechanisms of mitophagy in yeast. (2010)
  • Author(s): Kondo-Okamoto N., Noda, N.N., Takahashi I., Matsunami, M., Inagaki, F., Ohsumi Y., Okamoto K.
  • Organizer: 3rd International Symposium on Protein Community.(招待講演(英語))
  • Place of Presentation: Nara, Japan.
  • Year and Date: 2010-09-15
• [Presentation] Molecular mechanisms of mitophagy in yeast (2010)
  • Author(s): Koji Okamoto
  • Organizer: 3rd International Symposium on Protein Community
  • Place of Presentation: ホテル日航奈良(招待講演)
  • Year and Date: 2010-09-15
• [Presentation] Molecular mechanism of mitophagy. (2010)
  • Author(s): 岡本浩二
  • Organizer: Gordon Research Conference on Mitochondria and Chloroplasts.(招待講演(英語))
  • Place of Presentation: Lucca, Italy.
  • Year and Date: 2010-07-14
• [Presentation] Molecular mechanism of mitophagy (2010)
  • Author(s): Koji Okamoto
  • Organizer: Gordon Research Conference on Mitochondria and Chloroplasts
  • Place of Presentation: Il Ciocco Hotel and Resort, Lucca(Italy)(招待講演)
  • Year and Date: 2010-07-14
• [Presentation] ミトコンドリア分解を司る選択的オートファジーの制御機構 (2010)
  • Author(s): 岡本浩二
  • Organizer: 第19回酵母合同シンポジウム(招待講演(日本語))
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] ミトコンドリア分解を司る選択的オートファジーの制御機構 (2010)
  • Author(s): 岡本浩二
  • Organizer: 第19回酵母合同シンポジウム
  • Place of Presentation: 東京大学(招待講演)
  • Year and Date: 2010-06-24
• [Presentation] Molecular mechanisms of mitophagy in yeast (2010)
  • Author(s): Koji Okamoto
  • Organizer: Gordon Research Conference on Autophagy in Stress, Development and Disease
  • Place of Presentation: Il Ciocco Hotel and Resort, Lucca(Italy)
  • Year and Date: 2010-04-28
• [Presentation] The novel protein Mtv1 mediates mitophagy, an autophagy-related membrane dynamics specific for mitochondria. (2009)
  • Author(s): 岡本浩二
  • Organizer: Nagoya University G-COE International Mini-Symposium.(招待講演(英語))
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2009-12-13
• [Presentation] Molecular basis of mitophagy, degradation of mitochondria via selective autophagy. (2009)
  • Author(s): 岡本浩二, 岡本徳子, 大隅良典
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会・合同大会,シンポジウム「オルガネラ・ダイナミクス-形成・分解と機能制御」(招待講演(英語))
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-09
• [Presentation] 選択的オートファジーによるミトコンドリア分解の分子機構 (2009)
  • Author(s): 岡本浩二, 岡本徳子, 高橋郁子, 大隅良典
  • Organizer: 第82回日本生化学会大会,シンポジウム「ミトコンドリアが語る細胞機能・病態研究の新たな潮流」(招待講演(日本語))
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 選択的オートファジーによるミトコンドリア分解の分子機構 (2009)
  • Author(s): 岡本浩二, 岡本徳子, 高橋郁子, 大隅良典
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートピアホテル,神戸
  • Year and Date: 2009-10-23
• [Presentation] Molecular basis of mitochondrial degradation via selective autophagy in yeast. (2009)
  • Author(s): 岡本浩二, 岡本徳子, 高橋郁子, 大隅良典
  • Organizer: 5th International Symposium on Autophagy.(招待講演(英語))
  • Place of Presentation: Otsu, Japan.
  • Year and Date: 2009-09-26
• [Presentation] Molecular basis of mitochondrial degradation via selective autophagy in yeast (2009)
  • Author(s): Koji Okamoto, Noriko-Kondo Okamoto, Ikuko Takahashi, Yoshinori Ohsumi
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Otsu Prince Hotel, Otsu
  • Year and Date: 2009-09-26
• [Presentation] 選択的ミトコンドリア・オートファジーの分子基盤 (2009)
  • Author(s): 岡本浩二, 岡本徳子, 大隅良典
  • Organizer: 平成21年度日本生化学会関東支部例会,シンポジウム「タンパク質制御研究の新展開」(招待講演(日本語))
  • Place of Presentation: 筑波
  • Year and Date: 2009-06-20
• [Presentation] 選択的ミトコンドリア・オートファジーの分子基盤 (2009)
  • Author(s): 岡本浩二, 岡本徳子, 大隅良典
  • Organizer: 平成21年度生化会関東支部例会
  • Place of Presentation: 筑波国際会陽議場,筑波
  • Year and Date: 2009-06-20
• [Presentation] Molecular basis of mitophagy, degradation of mitochondria via autophagy-related membrane dynamics in yeast (2009)
  • Author(s): 岡本浩二
  • Organizer: Keystone Symposia X6 2009 Mitochondrial Dynamics and Physiology
  • Place of Presentation: Whistler, Canada
  • Year and Date: 2009-03-26
• [Presentation] Molecular basis of mitophagy, degradation of mitochondria via selective autophagy (2008)
  • Author(s): 岡本浩二
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアインド
  • Year and Date: 2008-12-09
• [Remarks]
  • URL: http://www.fbs.osaka-u.ac.jp/labs/okamoto/Okamoto_Lab/