Single motor mechanics of bacterial flagellar motor

• Project/Area Number: 20570147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: INOUE Yuichi 東北大学, 多元物質科学研究所, 助教 (50323499)
• Co-Investigator(Renkei-kenkyūsha): ISHIJIMA Akihiko 東北大学, 多元物質科学研究所, 教授 (80301216) ; FUKUOKA Hajime 東北大学, 多元物質科学研究所, 助教 (50447190)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生物物理 / 分子モーター / マイクロ・ナノデバイス / ナノバイオ

Research Abstract

High speed measurement of rotation of the bacterial flagellar motor was made to understand the molecular mechanism of force generation. Using a new system for temperature control, we found the torque steps induced by the heating and the recovery steps by restoring temperature, suggesting dynamics of torque-generating units in a motor.

Research Products

• [Journal Article] Ishijima, Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell (2011)
  • Author(s): Shun Terasawa, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Hiroto Takahashi, and Akihiko
  • Journal Title: Biophysical Journal Volume: vol 100 Pages: 2193-2200
  • Peer Reviewed
• [Journal Article] wo methods of temperature control for single-molecule measurements (2011)
  • Author(s): Matthew A. B. Baker, Yuichi Inoue, Kuniaki Takeda, Akihiko Ishijima, Richard M. Berry, T
  • Journal Title: European Biophysics Journal Volume: vol 40 Pages: 651-660
  • Peer Reviewed
• [Journal Article] Two methods of temperature control for single-molecule measurements (2011)
  • Author(s): Matthew A.B.Baker
  • Journal Title: European Biophysics Journal Volume: 40 Pages: 651-660
  • Peer Reviewed
• [Journal Article] Adhesion Effects of a Guanidinium Ion Appended Dendritic"Molecular Glue" on the ATP-Driven Sliding Motion of Actomyosin (2010)
  • Author(s): Kou Okuro, Kazushi Kinbara, Kuniaki Takeda, Yuichi Inoue, Akihiko Ishijima, Takuzo Aida
  • Journal Title: Angewandte Chemie, International Edition Volume: vol 49 Pages: 3030-3033
  • Peer Reviewed
• [Journal Article] Exchange of rotor components in functioning bacterial flagellar motor (2010)
  • Author(s): Hajime Fukuoka, Yuichi Inoue, Shun Terasawa, Hiroto Takahashi, Akihiko Ishijima
  • Journal Title: Biochemical and Biophysical Research Communications Volume: vol 394 Pages: 130-135
  • Peer Reviewed
• [Journal Article] Thermosensing Function of the Escherichia coli Redox Sensor Aer (2010)
  • Author(s): So-ichiro Nishiyama, Shinji Ohno, Noriko Ohta, Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima, Ikuro Kawagishi
  • Journal Title: Journal of Bacteriology Volume: vol 192 Pages: 1740-1743
  • Peer Reviewed
• [Journal Article] Verification of Single-Molecule Imaging and Single-Molecule Measurements (2010)
  • Author(s): Akihiko Ishijima, Hajime Fukuoka, Yuichi Inoue
  • Journal Title: Journal of Robotics and Mechatronics Volume: vol 22 Pages: 568-578
  • Peer Reviewed
• [Journal Article] Verification of Single-Molecule Imaging and Single-Molecule Measurements (2010)
  • Author(s): Akihiko Ishijima
  • Journal Title: Journal of Robotics and Mechatronics Volume: 22 Pages: 568-578
  • Peer Reviewed
• [Journal Article] Thermosensing Function of the Escherichia coli Redox Sensor Aer. (2010)
  • Author(s): So-ichiro Nishiyama
  • Journal Title: Journal of Bacteriology 192 Pages: 1740-1743
  • Peer Reviewed
• [Journal Article] Exchange of rotor components in functioning bacterial flagellar motor. (2010)
  • Author(s): Hajime Fukuoka
  • Journal Title: Biochemical and Biophysical Research Communications 394 Pages: 130-135
  • Peer Reviewed
• [Journal Article] Adhesion Effects of a Guanidinium Ion Appended Dendritic "Molecular Glue" on the ATP-Driven Sliding Motion of Actomyosin. (2010)
  • Author(s): Kou Okuro
  • Journal Title: Angewandte Chemie International Edition 49 Pages: 3030-3033
  • Peer Reviewed
• [Journal Article] ナトリウム駆動型キメラべん毛モーターのトルク・スピード関係 (2009)
  • Author(s): 井上裕一
  • Journal Title: 生物物理 49 Pages: 292-293
  • NAID: 10025975995
  • Peer Reviewed
• [Journal Article] Torque-Speed Relationships of Na+-driven Chimeric Flagellar Motors in Escherichia coli (2008)
  • Author(s): Yuichi Inoue, Chien-Jung Lo, Hajime Fukuoka, Hiroto Takahashi, Yoshiyuki Sowa, Teuta Pilizota, George H. Wadhams, Michio Homma, Richard M. Berry, Akihiko Ishijima
  • Journal Title: Journal of Molecular Biology Volume: vol 376 Pages: 1251-1259
  • Peer Reviewed
• [Journal Article] Torque-Speed Relationships of Na^+-driven Chimeric Flagellar Motors in Escherichia coli (2008)
  • Author(s): Yuichi Inoue
  • Journal Title: Journal of Molecular Biology 376 Pages: 1251-1259
  • Peer Reviewed
• [Presentation] バクテリアべん毛モーター運動の光制御 (2011)
  • Author(s): 井上裕一
  • Organizer: FIRST川合プロジェクト2010年度末報告会
  • Place of Presentation: 東京
  • Year and Date: 2011-02-18
• [Presentation] Torque steps of the bacterial flagellar motor induced by temperature control (2011)
  • Author(s): Yuichi Inoue
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
  • Place of Presentation: 京都
  • Year and Date: 2011-01-25
• [Presentation] Torque steps of the bacterial flagellar motor induced by temperature control (2011)
  • Author(s): Yuichi Inoue
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
  • Place of Presentation: Japan, Kyoto
  • Year and Date: 2011-01-25
• [Presentation] Torque steps of the bacterial flagellar motor induced by heating (2011)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: BLAST XI meeting (Bacterial Locomotion and Signal Transduction
  • Place of Presentation: USA, New Orleans
  • Year and Date: 2011-01-21
• [Presentation] Torque steps of the bacterial flagellar motor induced by heating (2011)
  • Author(s): Yuichi Inoue
  • Organizer: BLAST XI meeting (Bacterial Locomotion and Signal Transduction)
  • Place of Presentation: USA, New Orleans
  • Year and Date: 2011-01-21
• [Presentation] 偏光による蛍光分子の配向検出のための顕微鏡構築 (2010)
  • Author(s): 福岡創
  • Organizer: 第36回日本生体エネルギー研究会・特定領域研究「革新的ナノバイオ」合同シンポジウム
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2010-11-19
• [Presentation] 生体分子モーター活性の局所的熱変調 (2010)
  • Author(s): 井上裕一, 福岡創, 石島秋彦
  • Organizer: 第36回日本生体エネルギー研究会・特定領域研究「革新的ナノバイオ」合同シンポジウム
  • Place of Presentation: 吹田市
  • Year and Date: 2010-11-18
• [Presentation] 生体分子モーター活性の局所的熱変調 (2010)
  • Author(s): 井上裕一
  • Organizer: 第36回日本生体エネルギー研究会・特定領域研究「革新的ナノバイオ」合同シンポジウム
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2010-11-18
• [Presentation] Analysis of torque steps of the bacterial flagellar motor induced by heating (2010)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] Construction of the optical system detecting the orientation of molecule from the polarization of fluorescence (2010)
  • Author(s): Takahiro Shimizu
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] Direct observation of carbon nanotube in solution by surfactant-terminated fluorescent labeling (2010)
  • Author(s): Hiroshi Matsutaka
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] Measurement of rotation of multiple flagellar motors on a single cell with chemotactic substrates (2010)
  • Author(s): Takashi Sagawa
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] Measurement of rotational behavior of flagellar motors in E.coli induced by caged serine (2010)
  • Author(s): Yu Kikushi
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] Single molecule measurement of actomyosin motors at low temperature (2010)
  • Author(s): Hiroyasu Aizawa
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-22
• [Presentation] 生物物理にとっての高速カメラ革新 (2010)
  • Author(s): 井上裕一
  • Organizer: 第48回日本生物物理学会年会ランチョンセミナー
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-20
• [Presentation] 生物物理にとっての高速カメラ革新「長時間記録システムを用いた高速イメージング」 (2010)
  • Author(s): 井上裕一
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-09-20
• [Presentation] Diffusional motion analysis of water-dispersible and uniform carbon nanotubes in solution for drug delivery application (2010)
  • Author(s): Takeru Okada
  • Organizer: 11th International Conference on the Science and Application of Nanotubes
  • Place of Presentation: Canada, Montreal
  • Year and Date: 2010-06-28
• [Presentation] バクテリアベん毛モーターの温度応答解析と局所的熱変調 (2010)
  • Author(s): 井上裕一
  • Organizer: 特定領域研究「膜超分子モーターの革新的ナノサイエンス」第5回班会議
  • Place of Presentation: 東京都
  • Year and Date: 2010-06-25
• [Presentation] バクテリアべん毛モーターの温度応答解析と局所的熱変調 (2010)
  • Author(s): 井上裕一, 福岡創, 石島秋彦
  • Organizer: 特定領域研究「膜超分子モーターの革新的ナノサイエンス」第5回班会議
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] Torque steps in the temperature response of the chimeric bacterial flagellar motor (2010)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: International Symposium of Joint Research Network on Advanced Materials and Devices 「彫」
  • Place of Presentation: 苫小牧市
  • Year and Date: 2010-03-25
• [Presentation] Torque steps in the temperature response of the chimeric bacterial flagellar motor (2010)
  • Author(s): 井上裕一
  • Organizer: International Symposium of Joint Research Network on Advanced Materials and Devices"彫:Chou"
  • Place of Presentation: 北海道苫小牧市
  • Year and Date: 2010-03-25
• [Presentation] キメラべん毛モーターの温度応答トルクステップ解析 (2010)
  • Author(s): 井上裕一
  • Organizer: べん毛研究交流会
  • Place of Presentation: 愛知県蒲郡市
  • Year and Date: 2010-03-16
• [Presentation] キメラべん毛モーターの温度応答トルクステップ解析 (2010)
  • Author(s): 井上裕一
  • Organizer: 2009年度べん毛研究交流会
  • Place of Presentation: 愛知県蒲郡市
  • Year and Date: 2010-03-15
• [Presentation] Torque response of bacterial flagellar motor induced by transient heating (2009)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: 生物物理学会第47回年会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-11-01
• [Presentation] Torque response of bacterial flagellar motor induced by transient heating (2009)
  • Author(s): 井上裕一
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-10-31
• [Presentation] Torque response of bacterial flagellar motor induced by reversible temperature change (2009)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
  • Place of Presentation: 京都市
  • Year and Date: 2009-09-08
• [Presentation] Torque response of bacterial flagellar motor induced by reversible temperature change (2009)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: International Symposium of post-silicon materials and devices research alliance project
  • Place of Presentation: 吹田市
  • Year and Date: 2009-09-05
• [Presentation] Torque response of bacterial flagellar motor induced by reversible temperature change (2009)
  • Author(s): 井上裕一
  • Organizer: International Symposium of post-silicon materials and devices research alliance project
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2009-09-05
• [Presentation] 生体モーター運動活性の局所的熱変調 (2009)
  • Author(s): 井上裕一, 福岡創, 石島秋彦
  • Organizer: 特定領域研究膜超分子モーターの革新的ナノサイエンス第4回班会議
  • Place of Presentation: 沖縄県名護市
  • Year and Date: 2009-06-19
• [Presentation] Torque response of the Na+-driven chimeric flagellar motor in E. coli induced by reversible temperature change (2009)
  • Author(s): Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
  • Organizer: 特定領域研究マルチスケール操作によるシステム細胞工学第7回公開シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2009-03-06
• [Presentation] Temperature-dependence of torque generation of the Na+-driven chimeric flagellar motor and visualization of the stator proteins in E. coli (2009)
  • Author(s): Akihiko Ishijima, Yuichi Inoue, Hajime Fukuoka
  • Organizer: Biophysical Society 53rd Annual Meeting
  • Place of Presentation: U. S. A., boston
  • Year and Date: 2009-03-04
• [Presentation] Torque response of the sodium-driven chimeric motor induced by reversible temperature change (2009)
  • Author(s): Yuichi Inoue
  • Organizer: BLAST X meeting
  • Place of Presentation: Cuernavaca (Mexico)
  • Year and Date: 2009-01-19
• [Presentation] Torque response of the sodium-driven chimeric flagellar motor in E. coli induced by reversible temperature change (2009)
  • Author(s): Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
  • Organizer: BLAST X (Bacterial Locomotion and Signal Transduction X)
  • Place of Presentation: Cuernavaca, Mexico
  • Year and Date: 2009-01-18
• [Presentation] 温度変化によるキメラべん毛モーターの回転トルク応答 (2009)
  • Author(s): 井上裕一, 武田訓昌, 福岡創, 高橋泰人, 石島秋彦
  • Organizer: 2009年生体運動研究合同班会議
  • Place of Presentation: 東京
  • Year and Date: 2009-01-11
• [Presentation] 温度変化によるキメラべん毛モーターの回転トルク応答 (2009)
  • Author(s): 井上裕一
  • Organizer: 2009年生体運動研究合同班会議
  • Place of Presentation: 東京都
  • Year and Date: 2009-01-11
• [Presentation] ナトリウム駆動型キメラべん毛モーターの温度依存性, 「ポストシリコン物質・デバイス創製基盤技術アライアンス」 (2008)
  • Author(s): 井上裕一, 武田訓昌, 福岡創, 高橋泰人, 石島秋彦
  • Organizer: 平成20年度成果報告会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-12-25
• [Presentation] ナトリウム駆動型キメラべん毛モーターの温度依存性 (2008)
  • Author(s): 井上裕一
  • Organizer: ポストシリコン物質・デバイス創製基盤技術アライアンス平成20年度成果報告会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-12-25
• [Presentation] Torque response of the Na+-driven chimeric flagellar motor in E. coli induced by reversible temperature change (2008)
  • Author(s): Yuichi Inoue, Kuniaki Takeda, Hajime Fukuoka, Hiroto Takahashi, Akihiko Ishijima
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 博多市
  • Year and Date: 2008-12-05
• [Presentation] Torque response of the Na^+-driven chimeric flagellar motor in E. coil induced by reversible temperature change (2008)
  • Author(s): Yuichi Inoue
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 博多市
  • Year and Date: 2008-12-05
• [Presentation] Torque response of the Na^+-driven chimeric motor induced by reversible temperature change (2008)
  • Author(s): Yuichi Inoue, Hajime Fukuoka, Akihiko Ishijima
  • Organizer: The Fourth Workshop of the UK-Japan Bionanotechnology Collaboration
  • Place of Presentation: 神戸市
  • Year and Date: 2008-09-17
• [Presentation] ナトリウム駆動型キメラべん毛モーターの温度依存性 (2008)
  • Author(s): 井上裕一
  • Organizer: 生物物理学会・東北支部会シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2008-08-30
• [Presentation] ナトリウム駆動型キメラべん毛モーター変異体の運動性 (2008)
  • Author(s): 井上裕一, Chien-Jung Lo, 福岡創, 高橋泰人, 曽和義幸, 本間道夫, Richard M. Berry, 石島秋彦
  • Organizer: 第5回東北大バイオサイエンスシンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2008-05-19
• [Book] 現代界面コロイド科学の辞典, GFPの基本的原理とその発展系 (2010)
  • Author(s): 石島秋彦, 井上裕一, 福岡創
  • Publisher: 丸善社
• [Book] 現代界面コロイド科学の辞典,GFPの基本的原理とその発展系,120-121 (2010)
  • Author(s): 石島秋彦
  • Total Pages: 272
  • Publisher: 丸善株式会社
• [Book] ナトリウム駆動型キメラべん毛モーターのトルク-スピード関係 (2009)
  • Author(s): 井上裕一, 福岡創, 石島秋彦
  • Publisher: 生物物理
• [Book] ナノイメージング第5章 (2008)
  • Author(s): 石島秋彦, 井上裕一, 福岡創, 田中裕人
  • Publisher: エヌ・ティエス社
• [Book] 実用精密位置決め技術事典 (2008)
  • Author(s): 石島秋彦, 井上裕一, 福岡創, 田中裕人, 曽和義幸
  • Publisher: 産業技術サービスセンター
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/ishijima/paper.html
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/ishijima/paper.html
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/ishijima/paper.html
• [Remarks]
  • URL: http://www.tagen.tohoku.ac.jp/labo/ishijima/paper.html